Detailed information of TA system TA120187

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1741953..1742167
• Replicon: chromosome
• Accession: NZ_CP037840
• Organism: Clostridioides difficile strain Cd5

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: -
• Locus tag: E1H28_RS08310
• Protein ID: WP_032508407.1
• Coordinates: 1741953..1742114 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1742034..1742167 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1H28_RS08275 (1737141)	1737141..1737431	+	291	WP_021358995.1	HK97 gp10 family phage protein	-
E1H28_RS08280 (1737541)	1737541..1738347	+	807	WP_021358994.1	hypothetical protein	-
E1H28_RS19880 (1738696)	1738696..1738821	+	126	WP_021358993.1	hypothetical protein	-
E1H28_RS08285 (1738800)	1738800..1739132	+	333	WP_021358992.1	hypothetical protein	-
E1H28_RS08290 (1739125)	1739125..1739301	+	177	WP_021358991.1	hypothetical protein	-
E1H28_RS08295 (1739303)	1739303..1740613	+	1311	WP_021358990.1	phage tail sheath family protein	-
E1H28_RS08300 (1740630)	1740630..1741100	+	471	WP_003429851.1	phage tail tube protein	-
E1H28_RS08305 (1741172)	1741172..1741612	+	441	WP_003429853.1	phage portal protein	-
E1H28_RS08310 (1741953)	1741953..1742114	+	162	WP_032508407.1	hypothetical protein	Toxin
- (1742034)	1742034..1742167	-	134	NuclAT_2	-	Antitoxin
- (1742034)	1742034..1742167	-	134	NuclAT_2	-	Antitoxin
- (1742034)	1742034..1742167	-	134	NuclAT_3	-	Antitoxin
- (1742034)	1742034..1742167	-	134	NuclAT_4	-	Antitoxin
- (1742512)	1742512..1742560	-	49	NuclAT_10	-	-
- (1742512)	1742512..1742560	-	49	NuclAT_6	-	-
- (1742512)	1742512..1742560	-	49	NuclAT_6	-	-
- (1742512)	1742512..1742560	-	49	NuclAT_8	-	-
- (1743539)	1743539..1743644	-	106	NuclAT_5	-	-
- (1743539)	1743539..1743644	-	106	NuclAT_5	-	-
- (1743539)	1743539..1743644	-	106	NuclAT_7	-	-
- (1743539)	1743539..1743644	-	106	NuclAT_9	-	-
E1H28_RS19685 (1744110)	1744110..1744247	+	138	WP_021359009.1	hypothetical protein	-
E1H28_RS08320 (1744495)	1744495..1745055	+	561	WP_021359010.1	phage lipoprotein	-
E1H28_RS08325 (1745098)	1745098..1745667	+	570	WP_021359011.1	zinc ribbon domain protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T120187 WP_032508407.1 NZ_CP037840:1741953-1742114 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCIICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T120187 NZ_CP037840:1741953-1742114 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCATAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin

Nucleotide

• Length: 134 bp

>AT120187 NZ_CP037840:c1742167-1742034 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Antitoxin