Detailed information of TA system TA120176

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 3384393..3384608
• Replicon: chromosome
• Accession: NZ_CP037839
• Organism: Clostridioides difficile strain Cd6

Toxin (Protein)

• Gene name: CD2889
• Uniprot ID: Q183X5
• Locus tag: E1H29_RS15530
• Protein ID: WP_011861731.1
• Coordinates: 3384465..3384608 (-)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	RCd12
Locus tag	-
Coordinates	3384393..3384539 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1H29_RS15515 (3380051)	3380051..3381205	+	1155	WP_003439713.1	GGDEF domain-containing protein	-
E1H29_RS15520 (3381286)	3381286..3383004	-	1719	WP_011861730.1	putative 2-aminoethylphosphonate ABC transporter permease subunit	-
E1H29_RS15525 (3383009)	3383009..3383731	-	723	WP_021362017.1	ATP-binding cassette domain-containing protein	-
E1H29_RS20050 (3383718)	3383718..3383855	-	138	WP_235698571.1	hypothetical protein	-
- (3384393)	3384393..3384539	+	147	NuclAT_0	-	Antitoxin
- (3384393)	3384393..3384539	+	147	NuclAT_0	-	Antitoxin
- (3384393)	3384393..3384539	+	147	NuclAT_0	-	Antitoxin
- (3384393)	3384393..3384543	+	151	NuclAT_1	-	-
E1H29_RS15530 (3384465)	3384465..3384608	-	144	WP_011861731.1	hypothetical protein	Toxin
E1H29_RS15535 (3384866)	3384866..3385054	+	189	WP_009898401.1	hypothetical protein	-
E1H29_RS15540 (3385301)	3385301..3386005	-	705	WP_011861732.1	hypothetical protein	-
E1H29_RS15545 (3386007)	3386007..3386438	-	432	WP_011861052.1	protein-export chaperone SecB	-
E1H29_RS15550 (3386456)	3386456..3387019	-	564	WP_021371073.1	hypothetical protein	-
E1H29_RS15555 (3387565)	3387565..3387861	-	297	WP_011861049.1	hypothetical protein	-
E1H29_RS15560 (3387878)	3387878..3388693	-	816	WP_011861048.1	N-acetylmuramoyl-L-alanine amidase	-
E1H29_RS15565 (3388690)	3388690..3388950	-	261	WP_011861047.1	phage holin family protein	-
E1H29_RS15570 (3388970)	3388970..3389200	-	231	WP_011861046.1	hemolysin XhlA family protein	-
E1H29_RS15575 (3389235)	3389235..3389417	-	183	WP_011861045.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5399.34 Da
• Isoelectric Point: 10.8277

>T120176 WP_011861731.1 NZ_CP037839:c3384608-3384465 [Clostridioides difficile]
MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNKKRH

Nucleotide

• Length: 144 bp

>T120176 NZ_CP037839:c3384608-3384465 [Clostridioides difficile]
ATGAGCGAATTTTTACTAGGAGTGTTAGCTAGTTTAACAGCTAGCTTTATTACATATATTATTTCCAGAAAAGTAAAAAG
CCACTCTGGCAGGAGTGACTTTGAGCTTGATGTAAAAATCAAGTTTAATAAAAAACGACATTAA

Antitoxin

Nucleotide

• Length: 147 bp

>AT120176 NZ_CP037839:3384393-3384539 [Clostridioides difficile]
GTAAATTCGTATCAAAAACAAAAAAAGAAACTACAATCTATTAGCCTAGAGTGAAGTTTCATTAACTAAATATTAATGTC
GTTTTTTATTAAACTTGATTTTTACATCAAGCTCAAAGTCACTCCTGCCAGAGTGGCTTTTTACTTT

Structures

Toxin

• AlphaFold DB: A0A069A3R1

Antitoxin