Detailed information of TA system TA120170

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2632443..2632645
• Replicon: chromosome
• Accession: NZ_CP037839
• Organism: Clostridioides difficile strain Cd6

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: E1H29_RS12290
• Protein ID: WP_004454589.1
• Coordinates: 2632493..2632645 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2632443..2632572 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1H29_RS12260 (2627491)	2627491..2627808	+	318	WP_009897465.1	hypothetical protein	-
E1H29_RS12265 (2627944)	2627944..2628408	+	465	WP_011861514.1	hypothetical protein	-
E1H29_RS12270 (2628736)	2628736..2628897	-	162	WP_004454578.1	hypothetical protein	-
E1H29_RS20005 (2628949)	2628949..2629344	-	396	WP_011861515.1	hypothetical protein	-
E1H29_RS20010 (2629397)	2629397..2629762	-	366	WP_011861516.1	Bro-N domain-containing protein	-
E1H29_RS12280 (2629882)	2629882..2630034	-	153	WP_021361915.1	hypothetical protein	-
E1H29_RS12285 (2631376)	2631376..2632299	-	924	WP_011861517.1	SHOCT domain-containing protein	-
- (2632443)	2632443..2632572	+	130	NuclAT_2	-	Antitoxin
- (2632443)	2632443..2632572	+	130	NuclAT_2	-	Antitoxin
- (2632443)	2632443..2632572	+	130	NuclAT_3	-	Antitoxin
E1H29_RS12290 (2632493)	2632493..2632645	-	153	WP_004454589.1	hypothetical protein	Toxin
E1H29_RS12295 (2632919)	2632919..2633242	-	324	WP_009897482.1	DUF6054 family protein	-
E1H29_RS12300 (2633278)	2633278..2633532	-	255	WP_004454592.1	hypothetical protein	-
E1H29_RS12305 (2633968)	2633968..2634486	-	519	Protein_2352	transposase	-
E1H29_RS12310 (2634776)	2634776..2635151	-	376	Protein_2353	BlaI/MecI/CopY family transcriptional regulator	-
E1H29_RS12315 (2635256)	2635256..2635633	-	378	WP_011861518.1	BlaI/MecI/CopY family transcriptional regulator	-
E1H29_RS12320 (2636437)	2636437..2636931	+	495	WP_011861519.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
E1H29_RS12325 (2637316)	2637316..2637490	+	175	Protein_2356	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T120170 WP_004454589.1 NZ_CP037839:c2632645-2632493 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T120170 NZ_CP037839:c2632645-2632493 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT120170 NZ_CP037839:2632443-2632572 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin