Detailed information of TA system TA120161

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2567143..2567345
• Replicon: chromosome
• Accession: NZ_CP037835
• Organism: Clostridioides difficile strain Cd7

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: E1H30_RS12105
• Protein ID: WP_004454589.1
• Coordinates: 2567193..2567345 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2567143..2567272 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1H30_RS12070 (2562253)	2562253..2562570	+	318	WP_009897465.1	hypothetical protein	-
E1H30_RS12075 (2562706)	2562706..2563170	+	465	WP_004454576.1	hypothetical protein	-
E1H30_RS19935 (2563199)	2563199..2563324	-	126	Protein_2313	hypothetical protein	-
E1H30_RS12080 (2563499)	2563499..2563660	-	162	WP_004454578.1	hypothetical protein	-
E1H30_RS12085 (2563712)	2563712..2564164	-	453	WP_021359435.1	hypothetical protein	-
E1H30_RS12090 (2564161)	2564161..2564526	-	366	WP_016729316.1	Bro-N domain-containing protein	-
E1H30_RS12095 (2564646)	2564646..2564798	-	153	WP_009897477.1	hypothetical protein	-
E1H30_RS12100 (2566115)	2566115..2566924	-	810	WP_018112791.1	hypothetical protein	-
- (2567143)	2567143..2567272	+	130	NuclAT_3	-	Antitoxin
- (2567143)	2567143..2567272	+	130	NuclAT_3	-	Antitoxin
- (2567143)	2567143..2567272	+	130	NuclAT_5	-	Antitoxin
E1H30_RS12105 (2567193)	2567193..2567345	-	153	WP_004454589.1	hypothetical protein	Toxin
E1H30_RS12110 (2567619)	2567619..2567942	-	324	WP_009897482.1	DUF6054 family protein	-
E1H30_RS12115 (2567978)	2567978..2568232	-	255	WP_004454592.1	hypothetical protein	-
E1H30_RS12120 (2568664)	2568664..2569182	-	519	Protein_2322	transposase	-
E1H30_RS12125 (2569472)	2569472..2569846	-	375	Protein_2323	BlaI/MecI/CopY family transcriptional regulator	-
E1H30_RS12130 (2569951)	2569951..2570325	-	375	WP_004454597.1	BlaI/MecI/CopY family transcriptional regulator	-
E1H30_RS12135 (2571129)	2571129..2571617	+	489	WP_004454599.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
E1H30_RS12140 (2571978)	2571978..2572176	+	199	Protein_2326	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T120161 WP_004454589.1 NZ_CP037835:c2567345-2567193 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T120161 NZ_CP037835:c2567345-2567193 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT120161 NZ_CP037835:2567143-2567272 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin