Detailed information of TA system TA120144

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2540727..2540929
• Replicon: chromosome
• Accession: NZ_CP037831
• Organism: Clostridioides difficile strain Cd8

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: E1H31_RS11875
• Protein ID: WP_004454589.1
• Coordinates: 2540777..2540929 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2540727..2540856 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1H31_RS11850 (2536169)	2536169..2536486	+	318	WP_004454574.1	hypothetical protein	-
E1H31_RS11855 (2536621)	2536621..2537085	+	465	WP_021370488.1	hypothetical protein	-
E1H31_RS19885 (2537114)	2537114..2537239	-	126	Protein_2269	hypothetical protein	-
E1H31_RS19890 (2537627)	2537627..2538079	-	453	WP_021370489.1	hypothetical protein	-
E1H31_RS19895 (2538076)	2538076..2538441	-	366	WP_021370490.1	Bro-N domain-containing protein	-
E1H31_RS11865 (2538561)	2538561..2538749	-	189	WP_004454585.1	virulence factor Srl	-
E1H31_RS11870 (2539661)	2539661..2540509	-	849	WP_021370491.1	SHOCT domain-containing protein	-
- (2540727)	2540727..2540856	+	130	NuclAT_1	-	Antitoxin
- (2540727)	2540727..2540856	+	130	NuclAT_1	-	Antitoxin
- (2540727)	2540727..2540856	+	130	NuclAT_1	-	Antitoxin
E1H31_RS11875 (2540777)	2540777..2540929	-	153	WP_004454589.1	hypothetical protein	Toxin
E1H31_RS11880 (2541202)	2541202..2541525	-	324	WP_009897482.1	DUF6054 family protein	-
E1H31_RS11885 (2541561)	2541561..2541830	-	270	WP_021362728.1	hypothetical protein	-
E1H31_RS11890 (2542123)	2542123..2542620	-	498	Protein_2277	transposase	-
E1H31_RS11895 (2542910)	2542910..2543285	-	376	Protein_2278	BlaI/MecI/CopY family transcriptional regulator	-
E1H31_RS11900 (2543390)	2543390..2543767	-	378	WP_021363605.1	BlaI/MecI/CopY family transcriptional regulator	-
E1H31_RS11905 (2544571)	2544571..2545065	+	495	WP_021364347.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
E1H31_RS11910 (2545450)	2545450..2545624	+	175	Protein_2281	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T120144 WP_004454589.1 NZ_CP037831:c2540929-2540777 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T120144 NZ_CP037831:c2540929-2540777 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT120144 NZ_CP037831:2540727-2540856 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin