Detailed information of TA system TA120126

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2810327..2810536
• Replicon: chromosome
• Accession: NZ_CP037827
• Organism: Clostridioides difficile strain Cd10

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: Q182K5
• Locus tag: E1H33_RS13245
• Protein ID: WP_004454815.1
• Coordinates: 2810378..2810536 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	2810327..2810461 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1H33_RS13230 (2805420)	2805420..2807048	+	1629	WP_004454813.1	aspartate 4-decarboxylase	-
E1H33_RS13235 (2807169)	2807169..2808164	+	996	WP_003431031.1	asparaginase	-
E1H33_RS19740 (2808353)	2808353..2808601	-	249	WP_231298879.1	BlaI/MecI/CopY family transcriptional regulator	-
- (2810327)	2810327..2810461	+	135	NuclAT_1	-	Antitoxin
- (2810327)	2810327..2810461	+	135	NuclAT_1	-	Antitoxin
- (2810327)	2810327..2810461	+	135	NuclAT_2	-	Antitoxin
E1H33_RS13245 (2810378)	2810378..2810536	-	159	WP_004454815.1	hypothetical protein	Toxin
E1H33_RS13250 (2810895)	2810895..2812265	-	1371	WP_009897709.1	cell wall-binding protein Cwp29	-
E1H33_RS19750 (2812701)	2812701..2812787	+	87	WP_231298880.1	hypothetical protein	-
E1H33_RS19755 (2812831)	2812831..2813130	-	300	WP_225722727.1	hypothetical protein	-
E1H33_RS13265 (2813158)	2813158..2813376	-	219	WP_172617957.1	hypothetical protein	-
E1H33_RS13270 (2813317)	2813317..2813589	+	273	WP_004454820.1	tyrosine-type recombinase/integrase	-
E1H33_RS13275 (2813907)	2813907..2814575	-	669	WP_009897711.1	VanZ family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5996.20 Da
• Isoelectric Point: 11.3102

>T120126 WP_004454815.1 NZ_CP037827:c2810536-2810378 [Clostridioides difficile]
MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T120126 NZ_CP037827:c2810536-2810378 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGCATACTAGCAAGTCTATCTGCTAGCTTAATAGTTTATATAGCTAGCAAACTATTTAGAAA
GCGTAAAAAACCACTCAAAGCGGCAACTAAGAGTGGTTGGGAATTTGATTTAAAAATCAGATTCCATAAAACTAAGTAA

Antitoxin

Nucleotide

• Length: 135 bp

>AT120126 NZ_CP037827:2810327-2810461 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Toxin

• AlphaFold DB: A0A125V849

Antitoxin