Detailed information of TA system TA120106

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2537129..2537331
• Replicon: chromosome
• Accession: NZ_CP037822
• Organism: Clostridioides difficile strain Cd11

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: E1H34_RS11875
• Protein ID: WP_004454589.1
• Coordinates: 2537179..2537331 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2537129..2537258 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1H34_RS11850 (2532570)	2532570..2532887	+	318	WP_004454574.1	hypothetical protein	-
E1H34_RS11855 (2533023)	2533023..2533487	+	465	WP_021370488.1	hypothetical protein	-
E1H34_RS20695 (2533516)	2533516..2533641	-	126	Protein_2258	hypothetical protein	-
E1H34_RS20700 (2534029)	2534029..2534481	-	453	WP_021370489.1	hypothetical protein	-
E1H34_RS20705 (2534478)	2534478..2534843	-	366	WP_021370490.1	Bro-N domain-containing protein	-
E1H34_RS11865 (2534963)	2534963..2535151	-	189	WP_004454585.1	virulence factor Srl	-
E1H34_RS11870 (2536063)	2536063..2536911	-	849	WP_021370491.1	SHOCT domain-containing protein	-
- (2537129)	2537129..2537258	+	130	NuclAT_1	-	Antitoxin
- (2537129)	2537129..2537258	+	130	NuclAT_1	-	Antitoxin
- (2537129)	2537129..2537258	+	130	NuclAT_1	-	Antitoxin
E1H34_RS11875 (2537179)	2537179..2537331	-	153	WP_004454589.1	hypothetical protein	Toxin
E1H34_RS11880 (2537604)	2537604..2537927	-	324	WP_009897482.1	DUF6054 family protein	-
E1H34_RS11885 (2537963)	2537963..2538232	-	270	WP_021362728.1	hypothetical protein	-
E1H34_RS11890 (2538525)	2538525..2539022	-	498	Protein_2266	transposase	-
E1H34_RS11895 (2539312)	2539312..2539687	-	376	Protein_2267	BlaI/MecI/CopY family transcriptional regulator	-
E1H34_RS11900 (2539792)	2539792..2540169	-	378	WP_021363605.1	BlaI/MecI/CopY family transcriptional regulator	-
E1H34_RS11905 (2540973)	2540973..2541467	+	495	WP_021364347.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
E1H34_RS11910 (2541828)	2541828..2542026	+	199	Protein_2270	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T120106 WP_004454589.1 NZ_CP037822:c2537331-2537179 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T120106 NZ_CP037822:c2537331-2537179 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT120106 NZ_CP037822:2537129-2537258 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin