Detailed information of TA system TA120094

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2539767..2539969
• Replicon: chromosome
• Accession: NZ_CP037821
• Organism: Clostridioides difficile strain Cd12

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: E1H35_RS11730
• Protein ID: WP_004454589.1
• Coordinates: 2539817..2539969 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2539767..2539896 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1H35_RS11705 (2535136)	2535136..2535600	+	465	WP_004454576.1	hypothetical protein	-
E1H35_RS18595 (2535629)	2535629..2535754	-	126	Protein_2231	hypothetical protein	-
E1H35_RS11710 (2535929)	2535929..2536090	-	162	WP_004454578.1	hypothetical protein	-
E1H35_RS18600 (2536286)	2536286..2536537	-	252	WP_004454580.1	hypothetical protein	-
E1H35_RS18605 (2536590)	2536590..2536955	-	366	WP_004454581.1	Bro-N domain-containing protein	-
E1H35_RS11720 (2537076)	2537076..2537228	-	153	WP_009897477.1	hypothetical protein	-
E1H35_RS11725 (2538700)	2538700..2539623	-	924	WP_004454587.1	SHOCT domain-containing protein	-
- (2539767)	2539767..2539896	+	130	NuclAT_1	-	Antitoxin
- (2539767)	2539767..2539896	+	130	NuclAT_1	-	Antitoxin
- (2539767)	2539767..2539896	+	130	NuclAT_1	-	Antitoxin
E1H35_RS11730 (2539817)	2539817..2539969	-	153	WP_004454589.1	hypothetical protein	Toxin
E1H35_RS11735 (2540243)	2540243..2540566	-	324	WP_009897482.1	DUF6054 family protein	-
E1H35_RS11740 (2540602)	2540602..2540856	-	255	WP_004454592.1	hypothetical protein	-
E1H35_RS11745 (2541288)	2541288..2541806	-	519	Protein_2240	transposase	-
E1H35_RS11750 (2542096)	2542096..2542470	-	375	Protein_2241	BlaI/MecI/CopY family transcriptional regulator	-
E1H35_RS11755 (2542575)	2542575..2542949	-	375	WP_004454597.1	BlaI/MecI/CopY family transcriptional regulator	-
E1H35_RS11760 (2543755)	2543755..2544243	+	489	WP_004454599.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
E1H35_RS11765 (2544628)	2544628..2544802	+	175	Protein_2244	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T120094 WP_004454589.1 NZ_CP037821:c2539969-2539817 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T120094 NZ_CP037821:c2539969-2539817 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT120094 NZ_CP037821:2539767-2539896 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin