Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) CD-RCd/-
Location 2651291..2651493 Replicon chromosome
Accession NZ_CP037818
Organism Clostridioides difficile strain Cd14

Toxin (Protein)


Gene name CD2299.1 Uniprot ID Q185H1
Locus tag E1H37_RS12760 Protein ID WP_004454589.1
Coordinates 2651341..2651493 (-) Length 51 a.a.

Antitoxin (RNA)


Gene name SQ1641
Locus tag -
Coordinates 2651291..2651420 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E1H37_RS12730 (2646540) 2646540..2646857 + 318 WP_009897465.1 hypothetical protein -
E1H37_RS12735 (2646993) 2646993..2647457 + 465 WP_004454576.1 hypothetical protein -
E1H37_RS19785 (2647486) 2647486..2647611 - 126 Protein_2443 hypothetical protein -
E1H37_RS12740 (2647786) 2647786..2647947 - 162 WP_004454578.1 hypothetical protein -
E1H37_RS19790 (2647999) 2647999..2648451 - 453 WP_009897470.1 hypothetical protein -
E1H37_RS19795 (2648448) 2648448..2648813 - 366 WP_009897471.1 Bro-N domain-containing protein -
E1H37_RS12750 (2648933) 2648933..2649085 - 153 WP_009897477.1 hypothetical protein -
E1H37_RS12755 (2650224) 2650224..2651147 - 924 WP_021364626.1 SHOCT domain-containing protein -
- (2651291) 2651291..2651420 + 130 NuclAT_4 - Antitoxin
- (2651291) 2651291..2651420 + 130 NuclAT_4 - Antitoxin
- (2651291) 2651291..2651420 + 130 NuclAT_7 - Antitoxin
E1H37_RS12760 (2651341) 2651341..2651493 - 153 WP_004454589.1 hypothetical protein Toxin
E1H37_RS12765 (2651767) 2651767..2652090 - 324 WP_021364628.1 DUF6054 family protein -
E1H37_RS12770 (2652126) 2652126..2652380 - 255 WP_004454592.1 hypothetical protein -
E1H37_RS12775 (2652812) 2652812..2653330 - 519 Protein_2452 transposase -
E1H37_RS19945 (2653620) 2653620..2653994 - 375 Protein_2453 BlaI/MecI/CopY family transcriptional regulator -
E1H37_RS12785 (2654099) 2654099..2654476 - 378 WP_021364350.1 BlaI/MecI/CopY family transcriptional regulator -
E1H37_RS12790 (2655281) 2655281..2655775 + 495 WP_021364347.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
E1H37_RS12795 (2656160) 2656160..2656334 + 175 Protein_2456 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-88)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5817.92 Da        Isoelectric Point: 10.6365

>T120089 WP_004454589.1 NZ_CP037818:c2651493-2651341 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Download         Length: 153 bp

>T120089 NZ_CP037818:c2651493-2651341 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin


Download         Length: 130 bp

>AT120089 NZ_CP037818:2651291-2651420 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A125V725

Antitoxin

Download structure file

References