Detailed information of TA system TA120071

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2607491..2607693
• Replicon: chromosome
• Accession: NZ_CP037812
• Organism: Clostridioides difficile strain Cd16

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: E1H39_RS12450
• Protein ID: WP_004454589.1
• Coordinates: 2607541..2607693 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2607491..2607620 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1H39_RS12420 (2602740)	2602740..2603057	+	318	WP_009897465.1	hypothetical protein	-
E1H39_RS12425 (2603193)	2603193..2603657	+	465	WP_004454576.1	hypothetical protein	-
E1H39_RS19490 (2603686)	2603686..2603811	-	126	Protein_2379	hypothetical protein	-
E1H39_RS12430 (2603986)	2603986..2604147	-	162	WP_004454578.1	hypothetical protein	-
E1H39_RS19495 (2604199)	2604199..2604651	-	453	WP_009897470.1	hypothetical protein	-
E1H39_RS19500 (2604648)	2604648..2605013	-	366	WP_009897471.1	Bro-N domain-containing protein	-
E1H39_RS12440 (2605133)	2605133..2605285	-	153	WP_009897477.1	hypothetical protein	-
E1H39_RS12445 (2606424)	2606424..2607347	-	924	WP_021364626.1	SHOCT domain-containing protein	-
- (2607491)	2607491..2607620	+	130	NuclAT_4	-	Antitoxin
- (2607491)	2607491..2607620	+	130	NuclAT_4	-	Antitoxin
- (2607491)	2607491..2607620	+	130	NuclAT_7	-	Antitoxin
E1H39_RS12450 (2607541)	2607541..2607693	-	153	WP_004454589.1	hypothetical protein	Toxin
E1H39_RS12455 (2607967)	2607967..2608290	-	324	WP_021364628.1	DUF6054 family protein	-
E1H39_RS12460 (2608326)	2608326..2608580	-	255	WP_004454592.1	hypothetical protein	-
E1H39_RS12465 (2609012)	2609012..2609530	-	519	Protein_2388	transposase	-
E1H39_RS19660 (2609820)	2609820..2610194	-	375	Protein_2389	BlaI/MecI/CopY family transcriptional regulator	-
E1H39_RS12475 (2610299)	2610299..2610676	-	378	WP_021364350.1	BlaI/MecI/CopY family transcriptional regulator	-
E1H39_RS12480 (2611481)	2611481..2611975	+	495	WP_021364347.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
E1H39_RS12485 (2612360)	2612360..2612534	+	175	Protein_2392	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T120071 WP_004454589.1 NZ_CP037812:c2607693-2607541 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T120071 NZ_CP037812:c2607693-2607541 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT120071 NZ_CP037812:2607491-2607620 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin