Detailed information of TA system TA120067

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1739252..1739466
• Replicon: chromosome
• Accession: NZ_CP037812
• Organism: Clostridioides difficile strain Cd16

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: E1H39_RS08405
• Protein ID: WP_003429855.1
• Coordinates: 1739252..1739413 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1739333..1739466 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1H39_RS08370 (1734439)	1734439..1734729	+	291	WP_021358995.1	HK97 gp10 family phage protein	-
E1H39_RS08375 (1734839)	1734839..1735645	+	807	WP_021358994.1	hypothetical protein	-
E1H39_RS08380 (1735994)	1735994..1736431	+	438	WP_021362375.1	hypothetical protein	-
E1H39_RS08385 (1736424)	1736424..1736600	+	177	WP_021362376.1	hypothetical protein	-
E1H39_RS08390 (1736602)	1736602..1737912	+	1311	WP_021368245.1	phage tail sheath family protein	-
E1H39_RS08395 (1737929)	1737929..1738399	+	471	WP_003429851.1	phage tail tube protein	-
E1H39_RS08400 (1738471)	1738471..1738911	+	441	WP_003429853.1	phage portal protein	-
E1H39_RS08405 (1739252)	1739252..1739413	+	162	WP_003429855.1	hypothetical protein	Toxin
- (1739333)	1739333..1739466	-	134	NuclAT_2	-	Antitoxin
- (1739333)	1739333..1739466	-	134	NuclAT_2	-	Antitoxin
- (1739333)	1739333..1739466	-	134	NuclAT_3	-	Antitoxin
- (1739333)	1739333..1739466	-	134	NuclAT_5	-	Antitoxin
- (1739811)	1739811..1739916	-	106	NuclAT_10	-	-
- (1739811)	1739811..1739916	-	106	NuclAT_5	-	-
- (1739811)	1739811..1739916	-	106	NuclAT_5	-	-
- (1739811)	1739811..1739916	-	106	NuclAT_8	-	-
E1H39_RS08410 (1740738)	1740738..1740911	+	174	WP_018112722.1	hypothetical protein	-
E1H39_RS08415 (1741035)	1741035..1741862	+	828	WP_021368246.1	hypothetical protein	-
E1H39_RS08420 (1741925)	1741925..1742101	+	177	WP_009894378.1	hypothetical protein	-
E1H39_RS08425 (1742514)	1742514..1742717	-	204	WP_021367646.1	helix-turn-helix transcriptional regulator	-
E1H39_RS19440 (1742961)	1742961..1743764	+	804	WP_021377655.1	ORF6N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T120067 WP_003429855.1 NZ_CP037812:1739252-1739413 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T120067 NZ_CP037812:1739252-1739413 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin

Nucleotide

• Length: 134 bp

>AT120067 NZ_CP037812:c1739466-1739333 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin