Detailed information of TA system TA120057

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1162795..1163009
• Replicon: chromosome
• Accession: NZ_CP037812
• Organism: Clostridioides difficile strain Cd16

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: E1H39_RS05545
• Protein ID: WP_003429855.1
• Coordinates: 1162795..1162956 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1162876..1163009 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1H39_RS05500 (1158135)	1158135..1158368	+	234	WP_003429842.1	hypothetical protein	-
E1H39_RS05505 (1158378)	1158378..1158779	+	402	WP_021379034.1	hypothetical protein	-
E1H39_RS05510 (1158773)	1158773..1159120	+	348	WP_003429844.1	hypothetical protein	-
E1H39_RS05515 (1159120)	1159120..1159545	+	426	WP_003429845.1	HK97 gp10 family phage protein	-
E1H39_RS05520 (1159538)	1159538..1159975	+	438	WP_009896628.1	hypothetical protein	-
E1H39_RS05525 (1159968)	1159968..1160144	+	177	WP_021395206.1	hypothetical protein	-
E1H39_RS05530 (1160145)	1160145..1161455	+	1311	WP_021368245.1	phage tail sheath family protein	-
E1H39_RS05535 (1161472)	1161472..1161942	+	471	WP_003429851.1	phage tail tube protein	-
E1H39_RS05540 (1162014)	1162014..1162454	+	441	WP_003429853.1	phage portal protein	-
E1H39_RS05545 (1162795)	1162795..1162956	+	162	WP_003429855.1	hypothetical protein	Toxin
- (1162876)	1162876..1163009	-	134	NuclAT_3	-	Antitoxin
- (1162876)	1162876..1163009	-	134	NuclAT_3	-	Antitoxin
- (1162876)	1162876..1163009	-	134	NuclAT_4	-	Antitoxin
- (1162876)	1162876..1163009	-	134	NuclAT_6	-	Antitoxin
- (1163354)	1163354..1163459	-	106	NuclAT_11	-	-
- (1163354)	1163354..1163459	-	106	NuclAT_6	-	-
- (1163354)	1163354..1163459	-	106	NuclAT_6	-	-
- (1163354)	1163354..1163459	-	106	NuclAT_9	-	-
E1H39_RS05550 (1164213)	1164213..1164401	+	189	WP_003429858.1	hypothetical protein	-
E1H39_RS05555 (1164520)	1164520..1165386	+	867	WP_021378510.1	BRO family protein	-
E1H39_RS05560 (1165439)	1165439..1165594	+	156	WP_021378513.1	hypothetical protein	-
E1H39_RS05565 (1166013)	1166013..1166225	-	213	WP_016728883.1	helix-turn-helix transcriptional regulator	-
E1H39_RS19420 (1166500)	1166500..1167279	+	780	WP_021391616.1	ORF6N domain-containing protein	-
E1H39_RS05580 (1167312)	1167312..1167464	+	153	WP_021378619.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T120057 WP_003429855.1 NZ_CP037812:1162795-1162956 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T120057 NZ_CP037812:1162795-1162956 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin

Nucleotide

• Length: 134 bp

>AT120057 NZ_CP037812:c1163009-1162876 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin