Detailed information of TA system TA120048

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2521169..2521371
• Replicon: chromosome
• Accession: NZ_CP037806
• Organism: Clostridioides difficile strain Cd18

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: E1H41_RS11805
• Protein ID: WP_004454589.1
• Coordinates: 2521219..2521371 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2521169..2521298 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1H41_RS11775 (2516614)	2516614..2516931	+	318	WP_009897465.1	hypothetical protein	-
E1H41_RS11780 (2517067)	2517067..2517531	+	465	WP_009897467.1	hypothetical protein	-
E1H41_RS11785 (2517859)	2517859..2518020	-	162	WP_004454578.1	hypothetical protein	-
E1H41_RS20530 (2518072)	2518072..2518524	-	453	WP_009897470.1	hypothetical protein	-
E1H41_RS20535 (2518521)	2518521..2518886	-	366	WP_009897471.1	Bro-N domain-containing protein	-
E1H41_RS11795 (2519006)	2519006..2519194	-	189	WP_004454585.1	virulence factor Srl	-
E1H41_RS11800 (2520141)	2520141..2520950	-	810	WP_018112791.1	hypothetical protein	-
- (2521169)	2521169..2521298	+	130	NuclAT_1	-	Antitoxin
- (2521169)	2521169..2521298	+	130	NuclAT_1	-	Antitoxin
- (2521169)	2521169..2521298	+	130	NuclAT_1	-	Antitoxin
E1H41_RS11805 (2521219)	2521219..2521371	-	153	WP_004454589.1	hypothetical protein	Toxin
E1H41_RS11810 (2521645)	2521645..2521968	-	324	WP_009897482.1	DUF6054 family protein	-
E1H41_RS11815 (2522004)	2522004..2522258	-	255	WP_004454592.1	hypothetical protein	-
E1H41_RS11820 (2522690)	2522690..2523208	-	519	Protein_2287	transposase	-
E1H41_RS20830 (2523498)	2523498..2523871	-	374	Protein_2288	BlaI/MecI/CopY family transcriptional regulator	-
E1H41_RS11830 (2523976)	2523976..2524350	-	375	WP_004454597.1	BlaI/MecI/CopY family transcriptional regulator	-
E1H41_RS11835 (2525152)	2525152..2525640	+	489	WP_009897487.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
E1H41_RS11840 (2526025)	2526025..2526199	+	175	Protein_2291	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-118)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T120048 WP_004454589.1 NZ_CP037806:c2521371-2521219 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T120048 NZ_CP037806:c2521371-2521219 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT120048 NZ_CP037806:2521169-2521298 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin