Detailed information of TA system TA120041

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1718162..1718376
• Replicon: chromosome
• Accession: NZ_CP037802
• Organism: Clostridioides difficile strain Cd23

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: E1H46_RS08055
• Protein ID: WP_003429855.1
• Coordinates: 1718162..1718323 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1718243..1718376 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1H46_RS08020 (1713350)	1713350..1713640	+	291	WP_021358995.1	HK97 gp10 family phage protein	-
E1H46_RS08025 (1713750)	1713750..1714556	+	807	WP_009888838.1	hypothetical protein	-
E1H46_RS08030 (1714905)	1714905..1715342	+	438	WP_009901504.1	hypothetical protein	-
E1H46_RS08035 (1715335)	1715335..1715511	+	177	WP_016728451.1	hypothetical protein	-
E1H46_RS08040 (1715512)	1715512..1716822	+	1311	WP_021363494.1	phage tail sheath family protein	-
E1H46_RS08045 (1716839)	1716839..1717309	+	471	WP_003429851.1	phage tail tube protein	-
E1H46_RS08050 (1717381)	1717381..1717821	+	441	WP_003429853.1	phage portal protein	-
E1H46_RS08055 (1718162)	1718162..1718323	+	162	WP_003429855.1	hypothetical protein	Toxin
- (1718243)	1718243..1718376	-	134	NuclAT_1	-	Antitoxin
- (1718243)	1718243..1718376	-	134	NuclAT_1	-	Antitoxin
- (1718243)	1718243..1718376	-	134	NuclAT_1	-	Antitoxin
- (1718243)	1718243..1718376	-	134	NuclAT_2	-	Antitoxin
- (1718721)	1718721..1718769	-	49	NuclAT_5	-	-
- (1718721)	1718721..1718769	-	49	NuclAT_5	-	-
- (1718721)	1718721..1718769	-	49	NuclAT_6	-	-
- (1718721)	1718721..1718769	-	49	NuclAT_8	-	-
- (1719682)	1719682..1719787	-	106	NuclAT_4	-	-
- (1719682)	1719682..1719787	-	106	NuclAT_4	-	-
- (1719682)	1719682..1719787	-	106	NuclAT_5	-	-
- (1719682)	1719682..1719787	-	106	NuclAT_7	-	-
E1H46_RS08060 (1720573)	1720573..1721133	+	561	WP_021359010.1	phage lipoprotein	-
E1H46_RS08065 (1721176)	1721176..1721745	+	570	WP_021359011.1	zinc ribbon domain protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T120041 WP_003429855.1 NZ_CP037802:1718162-1718323 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T120041 NZ_CP037802:1718162-1718323 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin

Nucleotide

• Length: 134 bp

>AT120041 NZ_CP037802:c1718376-1718243 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin