Detailed information of TA system TA120026

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1852250..1852465
• Replicon: chromosome
• Accession: NZ_CP037797
• Organism: Clostridioides difficile strain Cd29

Toxin (Protein)

• Gene name: CD2889
• Uniprot ID: Q183X5
• Locus tag: E1H52_RS08550
• Protein ID: WP_011861731.1
• Coordinates: 1852322..1852465 (-)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	RCd12
Locus tag	-
Coordinates	1852250..1852396 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1H52_RS08515 (1847745)	1847745..1848503	-	759	WP_009888882.1	PTS sugar transporter subunit IIC	-
E1H52_RS08520 (1848520)	1848520..1848990	-	471	WP_003428544.1	PTS sugar transporter subunit IIB	-
E1H52_RS08525 (1848990)	1848990..1849733	-	744	WP_003437997.1	BtpA/SgcQ family protein	-
E1H52_RS08530 (1849737)	1849737..1850150	-	414	WP_009896035.1	PTS sugar transporter subunit IIA	-
E1H52_RS08535 (1850176)	1850176..1850751	-	576	WP_021360401.1	GntR family transcriptional regulator	-
E1H52_RS08540 (1850945)	1850945..1851328	-	384	WP_015984867.1	BlaI/MecI/CopY family transcriptional regulator	-
E1H52_RS08545 (1851438)	1851438..1851815	-	378	WP_021391346.1	BlaI/MecI/CopY family transcriptional regulator	-
- (1852250)	1852250..1852396	+	147	NuclAT_0	-	Antitoxin
- (1852250)	1852250..1852396	+	147	NuclAT_0	-	Antitoxin
- (1852250)	1852250..1852396	+	147	NuclAT_0	-	Antitoxin
- (1852250)	1852250..1852400	+	151	NuclAT_2	-	-
E1H52_RS08550 (1852322)	1852322..1852465	-	144	WP_011861731.1	hypothetical protein	Toxin
E1H52_RS08555 (1852723)	1852723..1852911	+	189	WP_009898401.1	hypothetical protein	-
E1H52_RS08560 (1853321)	1853321..1853887	-	567	WP_016056345.1	hypothetical protein	-
E1H52_RS08565 (1853889)	1853889..1854380	-	492	WP_016056344.1	hypothetical protein	-
E1H52_RS08570 (1854630)	1854630..1855442	-	813	WP_016056343.1	N-acetylmuramoyl-L-alanine amidase	-
E1H52_RS08575 (1855442)	1855442..1855699	-	258	WP_009898413.1	phage holin family protein	-
E1H52_RS08580 (1855719)	1855719..1855949	-	231	WP_021364192.1	hemolysin XhlA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5399.34 Da
• Isoelectric Point: 10.8277

>T120026 WP_011861731.1 NZ_CP037797:c1852465-1852322 [Clostridioides difficile]
MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNKKRH

Nucleotide

• Length: 144 bp

>T120026 NZ_CP037797:c1852465-1852322 [Clostridioides difficile]
ATGAGCGAATTTTTACTAGGAGTGTTAGCTAGTTTAACAGCTAGCTTTATTACATATATTATTTCCAGAAAAGTAAAAAG
CCACTCTGGCAGGAGTGACTTTGAGCTTGATGTAAAAATCAAGTTTAATAAAAAACGACATTAA

Antitoxin

Nucleotide

• Length: 147 bp

>AT120026 NZ_CP037797:1852250-1852396 [Clostridioides difficile]
GTAAATTCGTATCAAAAACAAAAAAAGAAACTACAATCTATTAGCCTAGAGTGAAGTTTCATTAACTAAATATTAATGTC
GTTTTTTATTAAACTTGATTTTTACATCAAGCTCAAAGTCACTCCTGCCAGAGTGGCTTTTTACTTT

Structures

Toxin

• AlphaFold DB: A0A069A3R1

Antitoxin