Detailed information of TA system TA120021

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 488413..488615
• Replicon: chromosome
• Accession: NZ_CP037797
• Organism: Clostridioides difficile strain Cd29

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: E1H52_RS02180
• Protein ID: WP_004454589.1
• Coordinates: 488413..488565 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	488486..488615 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1H52_RS02150 (484133)	484133..484627	-	495	WP_021364347.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
E1H52_RS02155 (485430)	485430..485807	+	378	WP_021364350.1	BlaI/MecI/CopY family transcriptional regulator	-
E1H52_RS19820 (485912)	485912..486286	+	375	Protein_430	BlaI/MecI/CopY family transcriptional regulator	-
E1H52_RS02165 (486576)	486576..487094	+	519	Protein_431	transposase	-
E1H52_RS02170 (487526)	487526..487780	+	255	WP_004454592.1	hypothetical protein	-
E1H52_RS02175 (487816)	487816..488139	+	324	WP_021364628.1	DUF6054 family protein	-
E1H52_RS02180 (488413)	488413..488565	+	153	WP_004454589.1	hypothetical protein	Toxin
- (488486)	488486..488615	-	130	NuclAT_3	-	Antitoxin
- (488486)	488486..488615	-	130	NuclAT_3	-	Antitoxin
- (488486)	488486..488615	-	130	NuclAT_5	-	Antitoxin
E1H52_RS02185 (488759)	488759..489682	+	924	WP_021364626.1	SHOCT domain-containing protein	-
E1H52_RS02190 (490821)	490821..490973	+	153	WP_009897477.1	hypothetical protein	-
E1H52_RS19350 (491093)	491093..491458	+	366	WP_009897471.1	Bro-N domain-containing protein	-
E1H52_RS19355 (491455)	491455..491907	+	453	WP_009897470.1	hypothetical protein	-
E1H52_RS02200 (491959)	491959..492120	+	162	WP_216168294.1	hypothetical protein	-
E1H52_RS19360 (492295)	492295..492420	+	126	Protein_440	hypothetical protein	-
E1H52_RS02205 (492449)	492449..492913	-	465	WP_004454576.1	hypothetical protein	-
E1H52_RS02210 (493049)	493049..493366	-	318	WP_009897465.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T120021 WP_004454589.1 NZ_CP037797:488413-488565 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T120021 NZ_CP037797:488413-488565 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT120021 NZ_CP037797:c488615-488486 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin