Detailed information of TA system TA120018

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 244290..244499
• Replicon: chromosome
• Accession: NZ_CP037797
• Organism: Clostridioides difficile strain Cd29

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: Q182K5
• Locus tag: E1H52_RS01050
• Protein ID: WP_004454815.1
• Coordinates: 244290..244448 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	244365..244499 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1H52_RS01025 (240251)	240251..240919	+	669	WP_009897711.1	VanZ family protein	-
E1H52_RS01030 (241237)	241237..241509	-	273	WP_004454820.1	tyrosine-type recombinase/integrase	-
E1H52_RS19325 (242039)	242039..242230	-	192	Protein_208	site-specific integrase	-
E1H52_RS01045 (242561)	242561..243931	+	1371	WP_009897709.1	cell wall-binding protein Cwp29	-
E1H52_RS01050 (244290)	244290..244448	+	159	WP_004454815.1	hypothetical protein	Toxin
- (244365)	244365..244499	-	135	NuclAT_2	-	Antitoxin
- (244365)	244365..244499	-	135	NuclAT_2	-	Antitoxin
- (244365)	244365..244499	-	135	NuclAT_4	-	Antitoxin
E1H52_RS19330 (245691)	245691..245939	+	249	WP_231298879.1	BlaI/MecI/CopY family transcriptional regulator	-
E1H52_RS01060 (246128)	246128..247123	-	996	WP_003431031.1	asparaginase	-
E1H52_RS01065 (247244)	247244..248872	-	1629	WP_004454813.1	aspartate 4-decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5996.20 Da
• Isoelectric Point: 11.3102

>T120018 WP_004454815.1 NZ_CP037797:244290-244448 [Clostridioides difficile]
MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T120018 NZ_CP037797:244290-244448 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGCATACTAGCAAGTCTATCTGCTAGCTTAATAGTTTATATAGCTAGCAAACTATTTAGAAA
GCGTAAAAAACCACTCAAAGCGGCAACTAAGAGTGGTTGGGAATTTGATTTAAAAATCAGATTCCATAAAACTAAGTAA

Antitoxin

Nucleotide

• Length: 135 bp

>AT120018 NZ_CP037797:c244499-244365 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Toxin

• AlphaFold DB: A0A125V849

Antitoxin