Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3868683..3869314 Replicon chromosome
Accession NZ_CP034931
Organism Shigella flexneri strain 2013C-3749

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag EPD86_RS19865 Protein ID WP_001259384.1
Coordinates 3869039..3869314 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag EPD86_RS19860 Protein ID WP_000593555.1
Coordinates 3868683..3869042 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EPD86_RS19835 (3864815) 3864815..3865759 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
EPD86_RS19840 (3865756) 3865756..3866589 + 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
EPD86_RS19845 (3866589) 3866589..3867353 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
EPD86_RS19850 (3867350) 3867350..3868156 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
EPD86_RS19855 (3868162) 3868162..3868563 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
EPD86_RS19860 (3868683) 3868683..3869042 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EPD86_RS19865 (3869039) 3869039..3869314 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
EPD86_RS19870 (3869373) 3869373..3870497 - 1125 WP_001216257.1 ABC transporter permease -
EPD86_RS19875 (3870497) 3870497..3873232 - 2736 WP_000149170.1 ribosome-associated ATPase/putative transporter RbbA -
EPD86_RS19880 (3873229) 3873229..3874296 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3868683..3888427 19744


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T116957 WP_001259384.1 NZ_CP034931:c3869314-3869039 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T116957 NZ_CP034931:c3869314-3869039 [Shigella flexneri]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT116957 WP_000593555.1 NZ_CP034931:c3869042-3868683 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT116957 NZ_CP034931:c3869042-3868683 [Shigella flexneri]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References