Detailed information of TA system TA115072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 635901..636532
• Replicon: chromosome
• Accession: NZ_CP034060
• Organism: Shigella flexneri strain FDAARGOS_535

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EGX88_RS04510
• Protein ID: WP_025760846.1
• Coordinates: 635901..636176 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: EGX88_RS04515
• Protein ID: WP_000593555.1
• Coordinates: 636173..636532 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX88_RS04495 (630919)	630919..631986	+	1068	WP_000361470.1	HlyD family secretion protein	-
EGX88_RS04500 (631983)	631983..634718	+	2736	WP_000149172.1	ribosome-associated ATPase/putative transporter RbbA	-
EGX88_RS04505 (634718)	634718..635842	+	1125	WP_001216257.1	ABC transporter permease	-
EGX88_RS04510 (635901)	635901..636176	+	276	WP_025760846.1	type II toxin-antitoxin system HicA family toxin	Toxin
EGX88_RS04515 (636173)	636173..636532	+	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EGX88_RS04520 (636652)	636652..637053	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
EGX88_RS04525 (637059)	637059..637865	-	807	WP_000173660.1	nickel import ATP-binding protein NikE	-
EGX88_RS04530 (637862)	637862..638626	-	765	WP_001136248.1	nickel import ATP-binding protein NikD	-
EGX88_RS04535 (638626)	638626..639459	-	834	WP_001008974.1	nickel ABC transporter permease subunit NikC	-
EGX88_RS04540 (639456)	639456..640400	-	945	WP_000947076.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP037923 Shigella flexneri 5a str. M90T Genomic island TA120378 4222298..4243335 0.002	inside	Genomic island	-	-	614719..636532	21813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-81)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10145.95 Da
• Isoelectric Point: 10.6469

>T115072 WP_025760846.1 NZ_CP034060:635901-636176 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKKGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T115072 NZ_CP034060:635901-636176 [Shigella flexneri]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGAAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT115072 WP_000593555.1 NZ_CP034060:636173-636532 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT115072 NZ_CP034060:636173-636532 [Shigella flexneri]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6