Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1849566..1850197 Replicon chromosome
Accession NZ_CP033510
Organism Shigella flexneri strain 2016AM-0877

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag EEL47_RS09575 Protein ID WP_001259384.1
Coordinates 1849922..1850197 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag EEL47_RS09570 Protein ID WP_000593555.1
Coordinates 1849566..1849925 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EEL47_RS09545 (1845698) 1845698..1846642 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
EEL47_RS09550 (1846639) 1846639..1847472 + 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
EEL47_RS09555 (1847472) 1847472..1848236 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
EEL47_RS09560 (1848233) 1848233..1849039 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
EEL47_RS09565 (1849045) 1849045..1849446 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
EEL47_RS09570 (1849566) 1849566..1849925 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EEL47_RS09575 (1849922) 1849922..1850197 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
EEL47_RS09580 (1850256) 1850256..1851380 - 1125 WP_001216257.1 ABC transporter permease -
EEL47_RS09585 (1851380) 1851380..1854115 - 2736 WP_000149170.1 ribosome-associated ATPase/putative transporter RbbA -
EEL47_RS09590 (1854112) 1854112..1855179 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1849566..1869310 19744


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T113780 WP_001259384.1 NZ_CP033510:c1850197-1849922 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T113780 NZ_CP033510:c1850197-1849922 [Shigella flexneri]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT113780 WP_000593555.1 NZ_CP033510:c1849925-1849566 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT113780 NZ_CP033510:c1849925-1849566 [Shigella flexneri]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References