Detailed information of TA system TA113414

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2044689..2044885
• Replicon: chromosome
• Accession: NZ_CP033216
• Organism: Clostridioides difficile strain CD21062

Toxin (Protein)

• Gene name: CD1418.2
• Uniprot ID: D5Q3X5
• Locus tag: EBL91_RS09880
• Protein ID: WP_003424190.1
• Coordinates: 2044733..2044885 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	CD630_n00500
Locus tag	-
Coordinates	2044689..2044789 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBL91_RS09865	2039956..2040213	-	258	WP_003424182.1	hypothetical protein	-
EBL91_RS09870	2040200..2040433	-	234	WP_003424184.1	hypothetical protein	-
EBL91_RS09875	2040621..2040809	+	189	WP_003424185.1	helix-turn-helix transcriptional regulator	-
-	2044689..2044789	+	101	NuclAT_0	-	Antitoxin
EBL91_RS09880	2044733..2044885	-	153	WP_003424190.1	hypothetical protein	Toxin
EBL91_RS19660	2045713..2046258	+	546	WP_077699404.1	site-specific integrase	-
EBL91_RS09895	2046803..2047648	+	846	WP_003424194.1	DUF4878 domain-containing protein	-
EBL91_RS09900	2047872..2048036	-	165	WP_003424195.1	hypothetical protein	-
EBL91_RS09905	2048568..2048899	-	332	Protein_1809	carboxymuconolactone decarboxylase family protein	-
EBL91_RS09910	2049159..2049800	-	642	WP_003424198.1	SdpI family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5744.78 Da
• Isoelectric Point: 10.6276

>T113414 WP_003424190.1 NZ_CP033216:c2044885-2044733 [Clostridioides difficile]
MDNFLQGILASLVASLIVYLTSKLFKKVKSHSGRSDFSFELEIKFKKNKH

Nucleotide

• Length: 153 bp

>T113414 NZ_CP033216:c2044885-2044733 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGTATATTAGCAAGTTTAGTTGCCAGTTTAATAGTTTACTTAACTAGTAAGTTATTTAAAAA
AGTAAAAAGCCACTCTGGCAGGAGTGACTTTAGTTTTGAACTAGAAATCAAGTTCAAAAAGAATAAACATTAG

Antitoxin

Nucleotide

• Length: 101 bp

>AT113414 NZ_CP033216:2044689-2044789 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTAGACGTAGAGTGAAGTTCAAATACTAATGTTTATTCTTTTTGAACTTGATTTCTAGTTC
AAAACTAAAGTCACTCCTGCC

Structures

Toxin

• AlphaFold DB: A0A449XCJ1

Antitoxin