Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3609067..3609698 Replicon chromosome
Accession NZ_CP032513
Organism Shigella flexneri strain SFL1520

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag D6T62_RS19100 Protein ID WP_001259385.1
Coordinates 3609067..3609342 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag D6T62_RS19105 Protein ID WP_000593555.1
Coordinates 3609339..3609698 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D6T62_RS19085 (3604085) 3604085..3605152 + 1068 WP_000361474.1 HlyD family secretion protein -
D6T62_RS19090 (3605149) 3605149..3607884 + 2736 WP_000149173.1 ribosome-associated ATPase/putative transporter RbbA -
D6T62_RS19095 (3607884) 3607884..3609008 + 1125 WP_005072005.1 ABC transporter permease -
D6T62_RS19100 (3609067) 3609067..3609342 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
D6T62_RS19105 (3609339) 3609339..3609698 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
D6T62_RS19110 (3609818) 3609818..3610219 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
D6T62_RS19115 (3610225) 3610225..3611031 - 807 WP_000173672.1 nickel import ATP-binding protein NikE -
D6T62_RS19120 (3611028) 3611028..3611792 - 765 WP_001136248.1 nickel import ATP-binding protein NikD -
D6T62_RS19125 (3611792) 3611792..3612625 - 834 WP_001008948.1 nickel ABC transporter permease subunit NikC -
D6T62_RS19130 (3612622) 3612622..3613566 - 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3593062..3609698 16636


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T111885 WP_001259385.1 NZ_CP032513:3609067-3609342 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T111885 NZ_CP032513:3609067-3609342 [Shigella flexneri]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACACCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT111885 WP_000593555.1 NZ_CP032513:3609339-3609698 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT111885 NZ_CP032513:3609339-3609698 [Shigella flexneri]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References