Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 2832628..2833355 | Replicon | chromosome |
| Accession | NZ_CP032513 | ||
| Organism | Shigella flexneri strain SFL1520 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A2Y4XNA6 |
| Locus tag | D6T62_RS15070 | Protein ID | WP_000547562.1 |
| Coordinates | 2833044..2833355 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | D6T62_RS15065 | Protein ID | WP_000126288.1 |
| Coordinates | 2832628..2833047 (-) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| D6T62_RS15050 (2828362) | 2828362..2829372 | - | 1011 | WP_005051459.1 | DNA-binding transcriptional regulator AscG | - |
| D6T62_RS15055 (2829632) | 2829632..2831080 | + | 1449 | WP_001107864.1 | PTS cellobiose/arbutin/salicin transporter subunit IIBC | - |
| D6T62_RS15060 (2831089) | 2831089..2832513 | + | 1425 | WP_005070582.1 | 6-phospho-beta-glucosidase AscB | - |
| D6T62_RS15065 (2832628) | 2832628..2833047 | - | 420 | WP_000126288.1 | helix-turn-helix domain-containing protein | Antitoxin |
| D6T62_RS15070 (2833044) | 2833044..2833355 | - | 312 | WP_000547562.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| D6T62_RS15075 (2833531) | 2833531..2834271 | - | 741 | Protein_2888 | hypothetical protein | - |
| D6T62_RS15080 (2834341) | 2834341..2834811 | - | 471 | WP_000132961.1 | hydrogenase maturation peptidase HycI | - |
| D6T62_RS15085 (2834804) | 2834804..2835214 | - | 411 | WP_001291916.1 | formate hydrogenlyase assembly protein HycH | - |
| D6T62_RS15090 (2835211) | 2835211..2835978 | - | 768 | WP_000067406.1 | formate hydrogenlyase subunit HycG | - |
| D6T62_RS15095 (2835978) | 2835978..2836520 | - | 543 | WP_000493794.1 | formate hydrogenlyase subunit HycF | - |
| D6T62_RS15100 (2836530) | 2836530..2838215 | - | 1686 | WP_005070598.1 | formate hydrogenlyase subunit HycE | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| flank | IS/Tn | - | - | 2827718..2828221 | 503 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12298.98 Da Isoelectric Point: 9.4924
>T111882 WP_000547562.1 NZ_CP032513:c2833355-2833044 [Shigella flexneri]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE
Download Length: 312 bp
>T111882 NZ_CP032513:c2833355-2833044 [Shigella flexneri]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAGGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAGGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15397.33 Da Isoelectric Point: 4.3648
>AT111882 WP_000126288.1 NZ_CP032513:c2833047-2832628 [Shigella flexneri]
MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL
MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT111882 NZ_CP032513:c2833047-2832628 [Shigella flexneri]
ATGACTGCTAATGCTGCACACGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA
ATGACTGCTAATGCTGCACACGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|