Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 250632..251347 Replicon chromosome
Accession NZ_CP032513
Organism Shigella flexneri strain SFL1520

Toxin (Protein)


Gene name yafO Uniprot ID A0A658YZ27
Locus tag D6T62_RS01160 Protein ID WP_001263492.1
Coordinates 250928..251347 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag D6T62_RS01155 Protein ID WP_000554759.1
Coordinates 250632..250925 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D6T62_RS01135 (246271) 246271..246768 + 498 WP_000006249.1 transposase -
D6T62_RS01140 (246985) 246985..248697 - 1713 Protein_223 flagellar biosynthesis protein FlhA -
D6T62_RS01145 (248669) 248669..249454 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
D6T62_RS01150 (249525) 249525..250580 + 1056 WP_001226172.1 DNA polymerase IV -
D6T62_RS01155 (250632) 250632..250925 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
D6T62_RS01160 (250928) 250928..251347 + 420 WP_001263492.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
D6T62_RS01165 (251381) 251381..252078 + 698 WP_223368647.1 IS1 family transposase -
D6T62_RS01170 (252112) 252112..252564 + 453 WP_001059863.1 GNAT family N-acetyltransferase -
D6T62_RS01175 (252810) 252810..253854 + 1045 Protein_230 RNA ligase RtcB family protein -
D6T62_RS01180 (253970) 253970..254530 + 561 Protein_231 peptide chain release factor H -
D6T62_RS01185 (254587) 254587..256044 - 1458 WP_001292992.1 cytosol nonspecific dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 251575..252078 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 16348.95 Da        Isoelectric Point: 8.0871

>T111865 WP_001263492.1 NZ_CP032513:250928-251347 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRFWVMLPTY

Download         Length: 420 bp

>T111865 NZ_CP032513:250928-251347 [Shigella flexneri]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGGG
TAATGCTGCCAACTTACTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT111865 WP_000554759.1 NZ_CP032513:250632-250925 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT111865 NZ_CP032513:250632-250925 [Shigella flexneri]
ATGCATCGGATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A658YZ27

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References