Detailed information of TA system TA105482

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2573652..2573854
• Replicon: chromosome
• Accession: NZ_CP029345
• Organism: Clostridioides difficile strain DSM 104452

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: CDIF104452_RS12205
• Protein ID: WP_004454589.1
• Coordinates: 2573702..2573854 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2573652..2573781 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF104452_RS12165 (2569160)	2569160..2569477	+	318	WP_009897465.1	hypothetical protein	-
CDIF104452_RS12170 (2569613)	2569613..2570077	+	465	WP_004454576.1	hypothetical protein	-
CDIF104452_RS12175 (2570106)	2570106..2570231	-	126	Protein_2321	hypothetical protein	-
CDIF104452_RS12180 (2570406)	2570406..2570567	-	162	WP_004454578.1	hypothetical protein	-
CDIF104452_RS12185 (2570619)	2570619..2571071	-	453	WP_009897470.1	hypothetical protein	-
CDIF104452_RS12190 (2571068)	2571068..2571433	-	366	WP_009897471.1	Bro-N domain-containing protein	-
CDIF104452_RS12195 (2571553)	2571553..2571705	-	153	WP_009897477.1	hypothetical protein	-
CDIF104452_RS12200 (2572585)	2572585..2573508	-	924	WP_021364626.1	SHOCT domain-containing protein	-
- (2573652)	2573652..2573781	+	130	NuclAT_2	-	Antitoxin
- (2573652)	2573652..2573781	+	130	NuclAT_2	-	Antitoxin
- (2573652)	2573652..2573781	+	130	NuclAT_3	-	Antitoxin
CDIF104452_RS12205 (2573702)	2573702..2573854	-	153	WP_004454589.1	hypothetical protein	Toxin
CDIF104452_RS12210 (2574128)	2574128..2574451	-	324	WP_021364628.1	DUF6054 family protein	-
CDIF104452_RS12215 (2574487)	2574487..2574741	-	255	WP_004454592.1	hypothetical protein	-
CDIF104452_RS12220 (2575173)	2575173..2575691	-	519	Protein_2330	transposase	-
CDIF104452_RS12225 (2575981)	2575981..2576355	-	375	Protein_2331	BlaI/MecI/CopY family transcriptional regulator	-
CDIF104452_RS12230 (2576460)	2576460..2576837	-	378	WP_021364350.1	BlaI/MecI/CopY family transcriptional regulator	-
CDIF104452_RS12235 (2577642)	2577642..2578136	+	495	WP_021364347.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
CDIF104452_RS12240 (2578521)	2578521..2578695	+	175	Protein_2334	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T105482 WP_004454589.1 NZ_CP029345:c2573854-2573702 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T105482 NZ_CP029345:c2573854-2573702 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT105482 NZ_CP029345:2573652-2573781 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin