Detailed information of TA system TA105474

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1158826..1159046
• Replicon: chromosome
• Accession: NZ_CP029345
• Organism: Clostridioides difficile strain DSM 104452

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: A0A069AVC0
• Locus tag: CDIF104452_RS05500
• Protein ID: WP_009901465.1
• Coordinates: 1158826..1158987 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1158907..1159046 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF104452_RS05450 (1153938)	1153938..1154171	+	234	WP_003429842.1	hypothetical protein	-
CDIF104452_RS05455 (1154181)	1154181..1154582	+	402	WP_015984842.1	hypothetical protein	-
CDIF104452_RS05460 (1154576)	1154576..1154923	+	348	WP_015984843.1	hypothetical protein	-
CDIF104452_RS05465 (1154923)	1154923..1155348	+	426	WP_009896626.1	HK97 gp10 family phage protein	-
CDIF104452_RS05470 (1155341)	1155341..1155778	+	438	WP_009901457.1	hypothetical protein	-
CDIF104452_RS05475 (1155771)	1155771..1155947	+	177	WP_021368348.1	hypothetical protein	-
CDIF104452_RS05480 (1155948)	1155948..1157258	+	1311	WP_015984844.1	phage tail sheath family protein	-
CDIF104452_RS05485 (1157280)	1157280..1157750	+	471	WP_015984845.1	phage tail tube protein	-
CDIF104452_RS05490 (1157803)	1157803..1158060	+	258	WP_015984846.1	hypothetical protein	-
CDIF104452_RS05495 (1158065)	1158065..1158505	+	441	WP_015984847.1	hypothetical protein	-
CDIF104452_RS05500 (1158826)	1158826..1158987	+	162	WP_009901465.1	hypothetical protein	Toxin
- (1158907)	1158907..1159046	-	140	NuclAT_5	-	Antitoxin
- (1158907)	1158907..1159046	-	140	NuclAT_5	-	Antitoxin
- (1158907)	1158907..1159046	-	140	NuclAT_7	-	Antitoxin
- (1158907)	1158907..1159046	-	140	NuclAT_8	-	Antitoxin
- (1159391)	1159391..1159496	-	106	NuclAT_3	-	-
- (1159391)	1159391..1159496	-	106	NuclAT_3	-	-
- (1159391)	1159391..1159496	-	106	NuclAT_4	-	-
- (1159391)	1159391..1159496	-	106	NuclAT_5	-	-
CDIF104452_RS05505 (1160185)	1160185..1160373	+	189	WP_003429858.1	hypothetical protein	-
CDIF104452_RS05510 (1160492)	1160492..1161358	+	867	WP_021378510.1	BRO family protein	-
CDIF104452_RS05515 (1161411)	1161411..1161566	+	156	WP_021378513.1	hypothetical protein	-
CDIF104452_RS05520 (1161985)	1161985..1162197	-	213	WP_016728883.1	helix-turn-helix transcriptional regulator	-
CDIF104452_RS05525 (1162472)	1162472..1163251	+	780	WP_021391616.1	ORF6N domain-containing protein	-
CDIF104452_RS05530 (1163284)	1163284..1163436	+	153	WP_021378619.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6034.28 Da
• Isoelectric Point: 11.0253

>T105474 WP_009901465.1 NZ_CP029345:1158826-1158987 [Clostridioides difficile]
MDNFLLSILASLIASLIGYIVCRCIKNVKSHSTRGKSKSGWELGFKIKFRKFK

Nucleotide

• Length: 162 bp

>T105474 NZ_CP029345:1158826-1158987 [Clostridioides difficile]
ATGGACAATTTTTTACTTAGTATATTAGCTAGCTTGATAGCTAGTTTAATTGGATATATCGTTTGTAGATGTATCAAAAA
CGTAAAAAGCCACTCTACTCGTGGCAAGAGTAAAAGTGGCTGGGAACTTGGTTTTAAAATAAAGTTCCGCAAATTTAAAT
AA

Antitoxin

Nucleotide

• Length: 140 bp

>AT105474 NZ_CP029345:c1159046-1158907 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTAGCGGTAGAGTGAAGTTCATAATTTTAAAAAATATAAATTATTTAAATTTGCGGAACTT
TATTTTAAAACCAAGTTCCCAGCCACTTTTACTCTTGCCACGAGTAGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVC0

Antitoxin