105287

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1690701..1691227 Replicon chromosome
Accession NZ_CP029239
Organism Escherichia coli strain BH212

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag DF195_RS08470 Protein ID WP_000323025.1
Coordinates 1690701..1690988 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag DF195_RS08475 Protein ID WP_000534858.1
Coordinates 1690988..1691227 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DF195_RS08425 1685725..1685940 - 216 WP_000839590.1 phage lysis protein EssD -
DF195_RS23200 1686160..1686330 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
DF195_RS08430 1686694..1686909 - 216 WP_000066484.1 cold shock-like protein CspB -
DF195_RS08435 1687210..1687422 + 213 WP_000087756.1 cold shock-like protein CspF -
DF195_RS08440 1687477..1687566 + 90 WP_120795389.1 hypothetical protein -
DF195_RS08445 1687844..1688596 - 753 WP_001047135.1 antitermination protein -
DF195_RS08450 1688610..1689659 - 1050 WP_001265198.1 DUF968 domain-containing protein -
DF195_RS08455 1689661..1689939 - 279 WP_012304870.1 hypothetical protein -
DF195_RS08460 1690006..1690257 - 252 WP_000980994.1 hypothetical protein -
DF195_RS08465 1690474..1690629 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
DF195_RS08470 1690701..1690988 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
DF195_RS08475 1690988..1691227 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
DF195_RS08480 1691252..1691557 + 306 WP_001326990.1 hypothetical protein -
DF195_RS08485 1691760..1692092 + 333 WP_001301033.1 protein FlxA -
DF195_RS08490 1692529..1692678 - 150 WP_011443592.1 hypothetical protein -
DF195_RS08495 1692713..1692991 - 279 Protein_1655 hypothetical protein -
DF195_RS08500 1692975..1693205 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
DF195_RS08505 1693289..1693696 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
DF195_RS08510 1693863..1694018 + 156 WP_000379575.1 DUF1391 family protein -
DF195_RS08515 1694178..1694396 + 219 WP_001171942.1 hypothetical protein -
DF195_RS08525 1694964..1695152 + 189 WP_000854559.1 cell division inhibition protein DicB -
DF195_RS08530 1695149..1695340 + 192 WP_001083297.1 lysis protein YdfD -
DF195_RS08535 1695433..1696200 + 768 Protein_1662 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1676357..1699399 23042


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T105287 WP_000323025.1 NZ_CP029239:c1690988-1690701 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T105287 NZ_CP029239:c1690988-1690701 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT105287 WP_000534858.1 NZ_CP029239:c1691227-1690988 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT105287 NZ_CP029239:c1691227-1690988 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References