Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1690701..1691227 Replicon chromosome
Accession NZ_CP029239
Organism Escherichia coli strain BH212

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag DF195_RS08470 Protein ID WP_000323025.1
Coordinates 1690701..1690988 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag DF195_RS08475 Protein ID WP_000534858.1
Coordinates 1690988..1691227 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DF195_RS08425 1685725..1685940 - 216 WP_000839590.1 phage lysis protein EssD -
DF195_RS23200 1686160..1686330 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
DF195_RS08430 1686694..1686909 - 216 WP_000066484.1 cold shock-like protein CspB -
DF195_RS08435 1687210..1687422 + 213 WP_000087756.1 cold shock-like protein CspF -
DF195_RS08440 1687477..1687566 + 90 WP_120795389.1 hypothetical protein -
DF195_RS08445 1687844..1688596 - 753 WP_001047135.1 antitermination protein -
DF195_RS08450 1688610..1689659 - 1050 WP_001265198.1 DUF968 domain-containing protein -
DF195_RS08455 1689661..1689939 - 279 WP_012304870.1 hypothetical protein -
DF195_RS08460 1690006..1690257 - 252 WP_000980994.1 hypothetical protein -
DF195_RS08465 1690474..1690629 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
DF195_RS08470 1690701..1690988 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
DF195_RS08475 1690988..1691227 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
DF195_RS08480 1691252..1691557 + 306 WP_001326990.1 hypothetical protein -
DF195_RS08485 1691760..1692092 + 333 WP_001301033.1 protein FlxA -
DF195_RS08490 1692529..1692678 - 150 WP_011443592.1 hypothetical protein -
DF195_RS08495 1692713..1692991 - 279 Protein_1655 hypothetical protein -
DF195_RS08500 1692975..1693205 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
DF195_RS08505 1693289..1693696 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
DF195_RS08510 1693863..1694018 + 156 WP_000379575.1 DUF1391 family protein -
DF195_RS08515 1694178..1694396 + 219 WP_001171942.1 hypothetical protein -
DF195_RS08525 1694964..1695152 + 189 WP_000854559.1 cell division inhibition protein DicB -
DF195_RS08530 1695149..1695340 + 192 WP_001083297.1 lysis protein YdfD -
DF195_RS08535 1695433..1696200 + 768 Protein_1662 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1676357..1699399 23042


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T105287 WP_000323025.1 NZ_CP029239:c1690988-1690701 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T105287 NZ_CP029239:c1690988-1690701 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT105287 WP_000534858.1 NZ_CP029239:c1691227-1690988 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT105287 NZ_CP029239:c1691227-1690988 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References