Detailed information of TA system TA105071

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 3021785..3022000
• Replicon: chromosome
• Accession: NZ_CP029154
• Organism: Clostridioides difficile strain CD161

Toxin (Protein)

• Gene name: CD2889
• Uniprot ID: Q183X5
• Locus tag: DDG63_RS21675
• Protein ID: WP_011861731.1
• Coordinates: 3021857..3022000 (-)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	RCd12
Locus tag	-
Coordinates	3021785..3021931 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDG63_RS14300	3017469..3017996	-	528	WP_003416006.1	hypoxanthine phosphoribosyltransferase	-
DDG63_RS14305	3018225..3019301	+	1077	WP_003427095.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
DDG63_RS14310	3019388..3020596	-	1209	WP_009893711.1	dicarboxylate/amino acid:cation symporter	-
DDG63_RS14315	3020947..3021330	-	384	WP_042741769.1	BlaI/MecI/CopY family transcriptional regulator	-
-	3021785..3021931	+	147	NuclAT_0	-	Antitoxin
-	3021785..3021935	+	151	NuclAT_1	-	-
DDG63_RS21675	3021857..3022000	-	144	WP_011861731.1	hypothetical protein	Toxin
DDG63_RS14325	3022262..3022450	+	189	WP_021360368.1	hypothetical protein	-
DDG63_RS14330	3022699..3023403	-	705	WP_021371074.1	hypothetical protein	-
DDG63_RS14335	3023405..3023836	-	432	WP_011861052.1	protein-export chaperone SecB	-
DDG63_RS14340	3023854..3024417	-	564	WP_021371073.1	hypothetical protein	-
DDG63_RS14345	3024963..3025259	-	297	WP_042741683.1	hypothetical protein	-
DDG63_RS14350	3025276..3026091	-	816	WP_032509862.1	N-acetylmuramoyl-L-alanine amidase	-
DDG63_RS14355	3026091..3026348	-	258	WP_015981519.1	phage holin family protein	-
DDG63_RS14360	3026368..3026598	-	231	WP_015981518.1	hemolysin XhlA family protein	-
DDG63_RS14365	3026634..3026816	-	183	WP_015981517.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3014855..3075622	60767

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5399.34 Da
• Isoelectric Point: 10.8277

>T105071 WP_011861731.1 NZ_CP029154:c3022000-3021857 [Clostridioides difficile]
MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNKKRH

Nucleotide

• Length: 144 bp

>T105071 NZ_CP029154:c3022000-3021857 [Clostridioides difficile]
ATGAGCGAATTTTTACTAGGAGTGTTAGCTAGTTTAACAGCTAGCTTTATTACATATATTATTTCCAGAAAAGTAAAAAG
CCACTCTGGCAGGAGTGACTTTGAGCTTGATGTAAAAATCAAGTTTAATAAAAAACGACATTAA

Antitoxin

Nucleotide

• Length: 147 bp

>AT105071 NZ_CP029154:3021785-3021931 [Clostridioides difficile]
GTAAATTCGTATCAAAAACAAAAAAAGAAACTACAATCTATTAGCCTAGAGTGAAGTTTCATTAACTAAATATTAATGTC
GTTTTTTATTAAACTTGATTTTTACATCAAGCTCAAAGTCACTCCTGCCAGAGTGGCTTTTTACTTT

Structures

Toxin

• AlphaFold DB: A0A069A3R1

Antitoxin