Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2503553..2504079 Replicon chromosome
Accession NZ_CP028703
Organism Escherichia coli strain ME8067

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag DBB47_RS13025 Protein ID WP_000323025.1
Coordinates 2503553..2503840 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag DBB47_RS13030 Protein ID WP_000534858.1
Coordinates 2503840..2504079 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBB47_RS12985 2498577..2498792 - 216 WP_000839590.1 phage lysis protein EssD -
DBB47_RS24240 2499012..2499182 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
DBB47_RS12990 2499546..2499761 - 216 WP_000066484.1 cold shock-like protein CspB -
DBB47_RS12995 2500062..2500274 + 213 WP_000087756.1 cold shock-like protein CspF -
DBB47_RS23975 2500329..2500418 + 90 WP_120795389.1 hypothetical protein -
DBB47_RS13000 2500696..2501448 - 753 WP_001047135.1 antitermination protein -
DBB47_RS13005 2501462..2502511 - 1050 WP_001393597.1 DUF968 domain-containing protein -
DBB47_RS13010 2502513..2502791 - 279 WP_012304870.1 hypothetical protein -
DBB47_RS13015 2502858..2503109 - 252 WP_000980994.1 hypothetical protein -
DBB47_RS13020 2503326..2503481 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
DBB47_RS13025 2503553..2503840 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
DBB47_RS13030 2503840..2504079 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
DBB47_RS13035 2504104..2504409 + 306 WP_001326990.1 hypothetical protein -
DBB47_RS13040 2504612..2504944 + 333 WP_001301033.1 protein FlxA -
DBB47_RS23980 2505381..2505530 - 150 WP_011443592.1 hypothetical protein -
DBB47_RS13050 2505565..2505843 - 279 Protein_2382 hypothetical protein -
DBB47_RS13055 2505827..2506057 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
DBB47_RS13060 2506141..2506548 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
DBB47_RS13065 2506715..2506870 + 156 WP_000379575.1 DUF1391 family protein -
DBB47_RS13075 2507030..2507248 + 219 WP_001171942.1 hypothetical protein -
DBB47_RS13085 2507816..2508004 + 189 WP_000854559.1 cell division inhibition protein DicB -
DBB47_RS13090 2508001..2508192 + 192 WP_001083297.1 lysis protein YdfD -
DBB47_RS13095 2508285..2509052 + 768 Protein_2389 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2489209..2510915 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T103002 WP_000323025.1 NZ_CP028703:c2503840-2503553 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T103002 NZ_CP028703:c2503840-2503553 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT103002 WP_000534858.1 NZ_CP028703:c2504079-2503840 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT103002 NZ_CP028703:c2504079-2503840 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References