Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2078674..2079200 Replicon chromosome
Accession NZ_CP028702
Organism Escherichia coli strain J53

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag DBB46_RS10910 Protein ID WP_000323025.1
Coordinates 2078674..2078961 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag DBB46_RS10915 Protein ID WP_000534858.1
Coordinates 2078961..2079200 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBB46_RS10870 2073698..2073913 - 216 WP_000839590.1 phage lysis protein EssD -
DBB46_RS24685 2074133..2074303 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
DBB46_RS10875 2074667..2074882 - 216 WP_000066484.1 cold shock-like protein CspB -
DBB46_RS10880 2075183..2075395 + 213 WP_000087756.1 cold shock-like protein CspF -
DBB46_RS24430 2075450..2075539 + 90 WP_120795389.1 hypothetical protein -
DBB46_RS10885 2075817..2076569 - 753 WP_001047135.1 antitermination protein -
DBB46_RS10890 2076583..2077632 - 1050 WP_001265198.1 DUF968 domain-containing protein -
DBB46_RS10895 2077634..2077912 - 279 WP_012304870.1 hypothetical protein -
DBB46_RS10900 2077979..2078230 - 252 WP_000980994.1 hypothetical protein -
DBB46_RS10905 2078447..2078602 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
DBB46_RS10910 2078674..2078961 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
DBB46_RS10915 2078961..2079200 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
DBB46_RS10920 2079225..2079530 + 306 WP_001326990.1 hypothetical protein -
DBB46_RS10925 2079733..2080065 + 333 WP_001301033.1 protein FlxA -
DBB46_RS24435 2080502..2080651 - 150 WP_011443592.1 hypothetical protein -
DBB46_RS10935 2080686..2080964 - 279 Protein_1999 hypothetical protein -
DBB46_RS10940 2080948..2081178 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
DBB46_RS10945 2081262..2081669 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
DBB46_RS10950 2081836..2081991 + 156 WP_000379575.1 DUF1391 family protein -
DBB46_RS10960 2082151..2082369 + 219 WP_001171942.1 hypothetical protein -
DBB46_RS10970 2082937..2083125 + 189 WP_000854559.1 cell division inhibition protein DicB -
DBB46_RS10975 2083122..2083313 + 192 WP_001083297.1 lysis protein YdfD -
DBB46_RS10980 2083406..2084173 + 768 Protein_2006 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2064330..2086036 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T102958 WP_000323025.1 NZ_CP028702:c2078961-2078674 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T102958 NZ_CP028702:c2078961-2078674 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT102958 WP_000534858.1 NZ_CP028702:c2079200-2078961 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT102958 NZ_CP028702:c2079200-2078961 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References