Detailed information of TA system TA101039

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2872018..2872227
• Replicon: chromosome
• Accession: NZ_CP028361
• Organism: Clostridioides difficile strain DSM 105001

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: Q182K5
• Locus tag: DSM105001_RS13660
• Protein ID: WP_004454815.1
• Coordinates: 2872069..2872227 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	2872018..2872152 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSM105001_RS13645	2867645..2869273	+	1629	WP_004454813.1	aspartate 4-decarboxylase	-
DSM105001_RS13650	2869394..2870389	+	996	WP_003431031.1	asparaginase	-
DSM105001_RS13655	2870578..2870961	-	384	Protein_2614	BlaI/MecI/CopY family transcriptional regulator	-
-	2872018..2872152	+	135	NuclAT_0	-	Antitoxin
DSM105001_RS13660	2872069..2872227	-	159	WP_004454815.1	hypothetical protein	Toxin
DSM105001_RS13665	2872586..2873956	-	1371	WP_009897709.1	cell wall-binding protein Cwp29	-
DSM105001_RS13670	2874239..2874337	+	99	Protein_2617	Arm DNA-binding domain-containing protein	-
DSM105001_RS13675	2874356..2874777	+	422	Protein_2618	hypothetical protein	-
DSM105001_RS13680	2875008..2875280	+	273	WP_004454820.1	tyrosine-type recombinase/integrase	-
DSM105001_RS13685	2875598..2876266	-	669	WP_009897711.1	VanZ family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-104)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5996.20 Da
• Isoelectric Point: 11.3102

>T101039 WP_004454815.1 NZ_CP028361:c2872227-2872069 [Clostridioides difficile]
MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T101039 NZ_CP028361:c2872227-2872069 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGCATACTAGCAAGTCTATCTGCTAGCTTAATAGTTTATATAGCTAGCAAACTATTTAGAAA
GCGTAAAAAACCACTCAAAGCGGCAACTAAGAGTGGTTGGGAATTTGATTTAAAAATCAGATTCCATAAAACTAAGTAA

Antitoxin

Nucleotide

• Length: 135 bp

>AT101039 NZ_CP028361:2872018-2872152 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Toxin

• AlphaFold DB: A0A125V849

Antitoxin