Detailed information of TA system TA101032

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1759600..1759814
• Replicon: chromosome
• Accession: NZ_CP028361
• Organism: Clostridioides difficile strain DSM 105001

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: DSM105001_RS08485
• Protein ID: WP_003429855.1
• Coordinates: 1759600..1759761 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1759681..1759814 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSM105001_RS08450	1754787..1755077	+	291	WP_021358995.1	HK97 gp10 family phage protein	-
DSM105001_RS08455	1755187..1755993	+	807	WP_021358994.1	hypothetical protein	-
DSM105001_RS08460	1756342..1756779	+	438	WP_021362375.1	hypothetical protein	-
DSM105001_RS08465	1756772..1756948	+	177	WP_021362376.1	hypothetical protein	-
DSM105001_RS08470	1756950..1758260	+	1311	WP_021368245.1	phage tail sheath family protein	-
DSM105001_RS08475	1758277..1758747	+	471	WP_003429851.1	phage tail tube protein	-
DSM105001_RS08480	1758819..1759259	+	441	WP_003429853.1	phage portal protein	-
DSM105001_RS08485	1759600..1759761	+	162	WP_003429855.1	hypothetical protein	Toxin
-	1759681..1759814	-	134	NuclAT_1	-	Antitoxin
-	1759681..1759814	-	134	NuclAT_2	-	Antitoxin
-	1760159..1760264	-	106	NuclAT_4	-	-
-	1760159..1760264	-	106	NuclAT_5	-	-
DSM105001_RS08490	1761086..1761259	+	174	WP_018112722.1	hypothetical protein	-
DSM105001_RS08495	1761383..1762210	+	828	WP_021368246.1	hypothetical protein	-
DSM105001_RS08500	1762273..1762449	+	177	WP_009894378.1	hypothetical protein	-
DSM105001_RS08505	1762862..1763065	-	204	WP_021367646.1	helix-turn-helix transcriptional regulator	-
DSM105001_RS08510	1763309..1763695	+	387	Protein_1587	ORF6N domain-containing protein	-
DSM105001_RS08515	1763759..1764112	+	354	WP_095890195.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1727564..1792362	64798

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T101032 WP_003429855.1 NZ_CP028361:1759600-1759761 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T101032 NZ_CP028361:1759600-1759761 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin

Nucleotide

• Length: 134 bp

>AT101032 NZ_CP028361:c1759814-1759681 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin