Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | timP-timR/- |
| Location | 2653356..2654126 | Replicon | chromosome |
| Accession | NC_000913 | ||
| Organism | Escherichia coli str. K-12 substr. MG1655 | ||
| T1TAdb ID | TA07145 | ||
Toxin (Protein)
| Gene name | timP | Uniprot ID | - |
| Locus tag | b4812 | Protein ID | YP_010283914.1 |
| Coordinates | 2654001..2654126 (+) | Length | 42 a.a. |
Antitoxin (RNA)
| Gene name | timR | ||
| Locus tag | - | ||
| Coordinates | 2653356..2653455 (-) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| b2521 | 2652494..2653339 | + | 846 | NP_417016.4 | 3-mercaptopyruvate sulfurtransferase | - |
| - | 2653356..2653455 | - | 100 | - | - | Antitoxin |
| b4812 | 2654001..2654126 | + | 126 | YP_010283914.1 | putative toxic protein TimP | Toxin |
| b2522 | 2654157..2654933 | - | 777 | NP_417017.4 | protein SseB | - |
| b2523 | 2655075..2656358 | - | 1284 | NP_417018.4 | aminopeptidase B | - |
| b2524 | 2656536..2656736 | - | 201 | NP_417019.1 | accessory iron-sulfur cluster assembly protein IscX | - |
| b2525 | 2656748..2657083 | - | 336 | NP_417020.1 | reduced ferredoxin | - |
| b2526 | 2657085..2658935 | - | 1851 | NP_417021.1 | iron-sulfur cluster biosynthesis chaperone HscA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 42 a.a. Molecular weight: 4477.28 Da Isoelectric Point: 8.5596
>T10087 YP_010283914.1 NC_000913:2654001-2654126 [Escherichia coli str. K-12 substr. MG1655]
MKIRCFCIVLIVSGALLTEVNNNRSLSGDNLLVVNNLQSSK
MKIRCFCIVLIVSGALLTEVNNNRSLSGDNLLVVNNLQSSK
Download Length: 126 bp
>T10087 NC_000913:2654001-2654126 [Escherichia coli str. K-12 substr. MG1655]
ATGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTC
CGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAA
ATGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTC
CGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAA
Antitoxin
Download Length: 100 bp
>AT10087 NC_000913:c2653455-2653356 [Escherichia coli str. K-12 substr. MG1655]
GCTGTGAAGCACCTGCGTTGCTCATGTAACACAAGTCAACCTGCTGGAAAGGCCGCGAACCAGACTAGCAATAAAAAACC
GCCAAATTCGGCGGTTTTTT
GCTGTGAAGCACCTGCGTTGCTCATGTAACACAAGTCAACCTGCTGGAAAGGCCGCGAACCAGACTAGCAATAAAAAACC
GCCAAATTCGGCGGTTTTTT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10086 | Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 |
48.718 |
100 |
0.5 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Liis Andresen et al. (2020) The Small Toxic Salmonella Protein TimP Targets the Cytoplasmic Membrane and Is Repressed by the Small RNA TimR. MBio 11(6):e01659-20. [PubMed:33172998]