Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | sprG-sprF/- |
| Location | 2206386..2206624 | Replicon | chromosome |
| Accession | NC_002745 | ||
| Organism | Staphylococcus aureus subsp. aureus N315 | ||
| T1TAdb ID | TA03806 | ||
Toxin (Protein)
| Gene name | SprG | Uniprot ID | - |
| Locus tag | SprG3 | Protein ID | - |
| Coordinates | 2206544..2206624 (-) | Length | 27 a.a. |
Antitoxin (RNA)
| Gene name | SprF | ||
| Locus tag | - | ||
| Coordinates | 2206386..2206549 (+) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SA_RS11210 (SA1953) | 2201983..2202360 | - | 378 | WP_000361059.1 | transposase | - |
| SA_RS11215 (SA1954) | 2202367..2204259 | - | 1893 | WP_001557544.1 | site-specific integrase | - |
| SA_RS11220 (SA1955) | 2204256..2205341 | - | 1086 | WP_000868132.1 | tyrosine-type recombinase/integrase | - |
| SA_RS11225 (SA1956) | 2205467..2206111 | + | 645 | Protein_2097 | transcriptional regulator | - |
| - | 2206386..2206549 | + | 164 | - | - | Antitoxin |
| - | 2206544..2206624 | - | 81 | - | - | Toxin |
| SA_RS14875 | 2207328..2207486 | + | 159 | WP_001792784.1 | hypothetical protein | - |
| SA_RS11235 (SA1957) | 2208144..2209001 | - | 858 | WP_000370925.1 | Cof-type HAD-IIB family hydrolase | - |
| SA_RS11240 (SA1958) | 2209069..2209851 | - | 783 | WP_000908182.1 | ABC transporter ATP-binding protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 27 a.a. Molecular weight: 2962.61 Da Isoelectric Point: 5.4974
>T10026 NC_002745:c2206624-2206544 [Staphylococcus aureus subsp. aureus N315]
MSDFEMLMVVLTIIGLVLISTQDHKK
MSDFEMLMVVLTIIGLVLISTQDHKK
Download Length: 81 bp
>T10026 NC_002745:c2206624-2206544 [Staphylococcus aureus subsp. aureus N315]
ATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGATTAGTACTCAAGACCATAAAAAATA
A
ATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGATTAGTACTCAAGACCATAAAAAATA
A
Antitoxin
Download Length: 164 bp
>AT10026 NC_002745:2206386-2206549 [Staphylococcus aureus subsp. aureus N315]
GTAGTAAGTAGAAGCAAAAGATGAAAATCTTTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAATGCCGGTCAAAGCGAATAGAAGGTT
ATTT
GTAGTAAGTAGAAGCAAAAGATGAAAATCTTTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAATGCCGGTCAAAGCGAATAGAAGGTT
ATTT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10147 | Staphylococcus aureus strain HG003 isolate RN1 Scaffold_3 |
100 |
100 |
1 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Marie-Laure Pinel-Marie et al. (2014) Dual toxic-peptide-coding Staphylococcus aureus RNA under antisense regulation targets host cells and bacterial rivals unequally. Cell Reports 7(2):424-435. [PubMed:24703849]
(2) Camille Riffaud et al. (2019) Functionality and cross-regulation of the four SprG/SprF type I toxin-antitoxin systems in Staphylococcus aureus. Nucleic Acids Research 47(4):1740-1758. [PubMed:30551143]