MGE detailed information
MGE type: Conjugative plasmid MGE length: 130351 bp
Accession: NZ_CP119732 Location: 1..130351 Organism: Escherichia coli strain E32 Replicon: plasmid pE32-2
Accession: NZ_CP119732 Location: 1..130351 Organism: Escherichia coli strain E32 Replicon: plasmid pE32-2
Gene structure
The 5 kb flanking regions of this MGE are displayed.
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| P1J21_RS25370 (2282) | 2282..3457 | - | 1176 | WP_002914189.1 | Efflux pump periplasmic linker BepF | VF |
| P1J21_RS25375 (3777) | 3777..4481 | + | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| P1J21_RS25380 (4816) | 4816..5208 | + | 393 | WP_000084745.1 | pyridoxamine 5'-phosphate oxidase family protein | - |
| P1J21_RS25385 (5528) | 5528..5914 | - | 387 | WP_063102497.1 | bleomycin binding protein | - |
| P1J21_RS25390 (6108) | 6108..6811 | - | 704 | Protein_5 | IS6 family transposase IS26 | Transposase |
| P1J21_RS25395 (6879) | 6879..8177 | + | 1299 | Protein_6 | Tn3 family transposase TnpA_Tn3 | Transposase |
| P1J21_RS25400 (8213) | 8213..9073 | - | 861 | Protein_7 | IS66 family transposase ISEc8 | Transposase |
| P1J21_RS25405 (9123) | 9123..9470 | - | 348 | WP_000612591.1 | IS66 family transposase ISEc8 | Transposase |
| P1J21_RS25410 (9467) | 9467..9847 | - | 381 | WP_001171523.1 | IS66 family transposase ISEc8 | Transposase |
| P1J21_RS25415 (9923) | 9923..10903 | - | 981 | WP_063099985.1 | NAD(P)-binding domain-containing protein | - |
| P1J21_RS25420 (10900) | 10900..11169 | - | 270 | WP_000379710.1 | SemiSWEET transporter | - |
| P1J21_RS25425 (11171) | 11171..11575 | - | 405 | WP_001389361.1 | hypothetical protein | - |
| P1J21_RS25430 (11547) | 11547..11990 | - | 444 | WP_001323891.1 | dehydrogenase | - |
| P1J21_RS25435 (12109) | 12109..12345 | + | 237 | WP_001323890.1 | colicin V immunity protein | - |
| P1J21_RS25440 (12323) | 12323..12634 | + | 312 | WP_001259758.1 | colicin V | - |
| P1J21_RS25445 (12804) | 12804..14900 | - | 2097 | WP_000184924.1 | microcin H47 export transporter peptidase/ATP-binding subunit MchF | - |
| P1J21_RS25450 (14893) | 14893..16167 | - | 1275 | WP_000489609.1 | HlyD family secretion protein | - |
| P1J21_RS25455 (16778) | 16778..17046 | + | 269 | Protein_18 | type II toxin-antitoxin system antitoxin YacA | - |
| P1J21_RS25460 (17046) | 17046..17171 | + | 126 | Protein_19 | type II toxin-antitoxin system toxin YacB | - |
| P1J21_RS25465 (17324) | 17324..17632 | + | 309 | WP_000377483.1 | DUF6404 family protein | - |
| P1J21_RS25470 (17710) | 17710..18011 | - | 302 | Protein_21 | hypothetical protein | - |
| P1J21_RS25475 (18192) | 18192..19226 | + | 1035 | WP_001190234.1 | 3-deoxy-7-phosphoheptulonate synthase | - |
| P1J21_RS25480 (20092) | 20092..22269 | + | 2178 | WP_001222186.1 | Ferric enterobactin receptor | VF |
| P1J21_RS25485 (22314) | 22314..23270 | - | 957 | WP_000271274.1 | hypothetical protein | VF |
| P1J21_RS25490 (23355) | 23355..24584 | - | 1230 | WP_000933678.1 | Enterochelin esterase | VF |
| P1J21_RS25495 (24688) | 24688..28347 | - | 3660 | WP_001389363.1 | Multidrug efflux ATP-binding/permease protein | VF |
| P1J21_RS25500 (28487) | 28487..29602 | - | 1116 | WP_001318220.1 | Elloramycin glycosyltransferase ElmGT | VF |
| P1J21_RS25505 (30913) | 30913..31937 | - | 1025 | Protein_28 | integrase core domain-containing protein | - |
| P1J21_RS25510 (32491) | 32491..32690 | + | 200 | Protein_29 | lysis protein | - |
| P1J21_RS25515 (32748) | 32748..33041 | - | 294 | WP_000738422.1 | increased serum survival lipoprotein Iss | - |
| P1J21_RS25520 (33666) | 33666..34186 | + | 521 | Protein_31 | IS3 family transposase IS2 | Transposase |
| P1J21_RS25530 (35418) | 35418..36155 | + | 738 | Protein_33 | IS3 family transposase IS2 | Transposase |
| P1J21_RS25535 (37397) | 37397..38590 | + | 1194 | WP_000450494.1 | GTP-binding protein | - |
| P1J21_RS25540 (38768) | 38768..38917 | + | 150 | Protein_35 | IS3 family transposase IS2 | Transposase |
| P1J21_RS25545 (39227) | 39227..40616 | + | 1390 | Protein_36 | ISNCY family transposase ISKpn21 | Transposase |
| P1J21_RS25550 (40851) | 40851..41792 | + | 942 | WP_000974762.1 | hypothetical protein | - |
| P1J21_RS25555 (41921) | 41921..42115 | - | 195 | Protein_38 | IS200-IS605 family transposase IS200C | Transposase |
| P1J21_RS25560 (42589) | 42589..43959 | - | 1371 | WP_001312828.1 | efflux transporter outer membrane subunit | - |
| P1J21_RS25565 (43963) | 43963..45903 | - | 1941 | WP_000733250.1 | Macrolide export ATP-binding/permease protein MacB | VF |
| P1J21_RS25570 (45900) | 45900..47087 | - | 1188 | WP_000928804.1 | efflux RND transporter periplasmic adaptor subunit | - |
| P1J21_RS25575 (47682) | 47682..48022 | - | 341 | Protein_42 | IS3 family transposase | - |
| P1J21_RS25580 (48068) | 48068..48393 | + | 326 | Protein_43 | IS3 family transposase IS911 | Transposase |
| P1J21_RS25585 (48541) | 48541..49740 | - | 1200 | Protein_44 | IS3 family transposase IS629 | Transposase |
| P1J21_RS25590 (49879) | 49879..50058 | + | 180 | Protein_45 | integrase core domain-containing protein | - |
| P1J21_RS25595 (51355) | 51355..52308 | + | 954 | WP_000280980.1 | Protease 7 | VF |
| P1J21_RS25600 (52741) | 52741..53850 | + | 1110 | WP_000771475.1 | SDR family oxidoreductase | - |
| P1J21_RS25605 (53919) | 53919..54821 | + | 903 | WP_001312822.1 | hypothetical protein | VF |
| P1J21_RS25610 (55195) | 55195..55383 | + | 189 | WP_001324033.1 | hypothetical protein | - |
| P1J21_RS25615 (55504) | 55504..56244 | + | 741 | WP_001066954.1 | tyrosine-type recombinase/integrase | - |
| P1J21_RS25620 (56529) | 56529..57506 | - | 978 | WP_000361611.1 | RepB family plasmid replication initiator protein | - |
| P1J21_RS25625 (58252) | 58252..58575 | + | 324 | Protein_52 | IS21 family transposase IS21 | Transposase |
| P1J21_RS25630 (58577) | 58577..58681 | + | 105 | Protein_53 | AAA family ATPase | - |
| P1J21_RS25635 (58742) | 58742..59255 | + | 514 | Protein_54 | DUF4113 domain-containing protein | - |
| P1J21_RS25645 (60488) | 60488..61402 | + | 915 | WP_000949004.1 | putative periplasmic iron-binding protein | VF |
| P1J21_RS25650 (61402) | 61402..62229 | + | 828 | WP_000983710.1 | Manganese transport system ATP-binding protein MntB | VF |
| P1J21_RS25655 (62226) | 62226..63083 | + | 858 | WP_001101723.1 | Manganese transport system membrane protein MntB | ARG |
| P1J21_RS25660 (63080) | 63080..63937 | + | 858 | WP_000968139.1 | Manganese transport system membrane protein MntB | ARG |
| P1J21_RS25665 (64171) | 64171..64428 | - | 258 | Protein_60 | Tn3 family transposase | - |
| P1J21_RS25670 (64426) | 64426..64839 | + | 414 | Protein_61 | phosphopyruvate hydratase | - |
| P1J21_RS25675 (65180) | 65180..65560 | + | 381 | WP_001318207.1 | fluoride efflux transporter CrcB | - |
| P1J21_RS25680 (65940) | 65940..67133 | - | 1194 | WP_000095526.1 | MFS transporter | - |
| P1J21_RS25685 (67269) | 67269..68993 | + | 1725 | WP_000602863.1 | N(2)-citryl-N(6)-acetyl-N(6)-hydroxylysine synthase | VF |
| P1J21_RS25690 (68994) | 68994..69941 | + | 948 | WP_000011910.1 | N(6)-hydroxylysine O-acetyltransferase | VF |
| P1J21_RS25695 (69941) | 69941..71683 | + | 1743 | WP_001015721.1 | Aerobactin synthase | VF |
| P1J21_RS25700 (71680) | 71680..73013 | + | 1334 | Protein_67 | L-lysine N6-monooxygenase | VF |
| P1J21_RS25705 (73039) | 73039..75240 | + | 2202 | WP_000973520.1 | Ferric aerobactin receptor | VF |
| P1J21_RS25710 (75725) | 75725..76422 | + | 698 | WP_094096600.1 | IS1 family transposase IS1S | Transposase |
| P1J21_RS25715 (76722) | 76722..77888 | + | 1167 | WP_001238646.1 | hypothetical protein | VF |
| P1J21_RS25720 (77888) | 77888..78859 | + | 972 | WP_000817028.1 | ParB/RepB/Spo0J family plasmid partition protein | - |
| P1J21_RS25725 (79496) | 79496..79665 | + | 170 | Protein_72 | hypothetical protein | - |
| P1J21_RS25730 (79848) | 79848..79928 | - | 81 | Protein_73 | hypothetical protein | - |
| P1J21_RS25735 (79998) | 79998..80204 | + | 207 | WP_000275856.1 | hypothetical protein | - |
| P1J21_RS25740 (80230) | 80230..80769 | + | 540 | WP_276170844.1 | single-stranded DNA-binding protein | - |
| P1J21_RS25745 (80837) | 80837..81070 | + | 234 | WP_000005987.1 | DUF905 family protein | - |
| P1J21_RS25750 (81098) | 81098..81295 | + | 198 | Protein_77 | hypothetical protein | - |
| P1J21_RS25755 (81350) | 81350..81784 | + | 435 | WP_000845936.1 | conjugation system SOS inhibitor PsiB | - |
| P1J21_RS25760 (81781) | 81781..82543 | + | 763 | Protein_79 | plasmid SOS inhibition protein A | - |
| P1J21_RS25765 (82512) | 82512..82700 | - | 189 | WP_001299721.1 | hypothetical protein | - |
| - (82512) | 82512..82736 | + | 225 | NuclAT_0 | - | Antitoxin Detail |
| - (82512) | 82512..82736 | + | 225 | NuclAT_0 | - | Antitoxin Detail |
| - (82512) | 82512..82736 | + | 225 | NuclAT_0 | - | Antitoxin Detail |
| - (82512) | 82512..82736 | + | 225 | NuclAT_0 | - | Antitoxin Detail |
| P1J21_RS25770 (82722) | 82722..82871 | + | 150 | Protein_81 | plasmid maintenance protein Mok | - |
| P1J21_RS25775 (82813) | 82813..82938 | + | 126 | WP_001372321.1 | type I toxin-antitoxin system Hok family toxin | Toxin Detail |
| P1J21_RS25780 (83158) | 83158..83388 | + | 231 | WP_071586998.1 | hypothetical protein | - |
| P1J21_RS25785 (83386) | 83386..83558 | - | 173 | Protein_84 | hypothetical protein | - |
| P1J21_RS25790 (83628) | 83628..83834 | + | 207 | WP_000547968.1 | hypothetical protein | - |
| P1J21_RS25795 (83859) | 83859..84146 | + | 288 | WP_000107535.1 | hypothetical protein | - |
| P1J21_RS25800 (84264) | 84264..85085 | + | 822 | WP_001234426.1 | DUF932 domain-containing protein | - |
| P1J21_RS25805 (85382) | 85382..85984 | - | 603 | WP_000243712.1 | hypothetical protein | VF |
| P1J21_RS25810 (86305) | 86305..86688 | + | 384 | WP_001151524.1 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| P1J21_RS25815 (86875) | 86875..87564 | + | 690 | WP_000283385.1 | conjugal transfer transcriptional regulator TraJ | - |
| P1J21_RS25820 (87657) | 87657..88058 | + | 402 | WP_001369361.1 | conjugal transfer relaxosome DNA-bindin protein TraY | - |
| P1J21_RS25825 (88091) | 88091..88456 | + | 366 | WP_000994779.1 | type IV conjugative transfer system pilin TraA | - |
| P1J21_RS25830 (88471) | 88471..88782 | + | 312 | WP_000012106.1 | type IV conjugative transfer system protein TraL | - |
| P1J21_RS25835 (88804) | 88804..89370 | + | 567 | WP_000399792.1 | type IV conjugative transfer system protein TraE | - |
| P1J21_RS25840 (89357) | 89357..90085 | + | 729 | WP_001230787.1 | type-F conjugative transfer system secretin TraK | - |
| P1J21_RS25845 (90085) | 90085..91512 | + | 1428 | WP_000146685.1 | F-type conjugal transfer pilus assembly protein TraB | - |
| P1J21_RS25850 (91502) | 91502..91756 | + | 255 | Protein_97 | conjugal transfer protein TraP | - |
| P1J21_RS25855 (91787) | 91787..94024 | + | 2238 | Protein_98 | type IV secretion system protein TraC | - |
| P1J21_RS25860 (94021) | 94021..94407 | + | 387 | WP_000099686.1 | type-F conjugative transfer system protein TrbI | - |
| P1J21_RS25865 (94404) | 94404..95036 | + | 633 | WP_001203720.1 | type-F conjugative transfer system protein TraW | - |
| P1J21_RS25870 (95033) | 95033..95995 | + | 963 | WP_276170843.1 | conjugal transfer pilus assembly protein TraU | - |
| P1J21_RS25875 (95988) | 95988..97196 | + | 1209 | Protein_102 | type-F conjugative transfer system mating-pair stabilization protein TraN | - |
| P1J21_RS25880 (97223) | 97223..97480 | + | 258 | WP_000864318.1 | conjugal transfer protein TrbE | - |
| P1J21_RS25885 (97473) | 97473..98216 | + | 744 | WP_001030393.1 | type-F conjugative transfer system pilin assembly protein TraF | - |
| P1J21_RS25890 (98232) | 98232..98579 | + | 348 | WP_001287910.1 | conjugal transfer protein TrbA | - |
| P1J21_RS25895 (98581) | 98581..98895 | - | 315 | WP_001324103.1 | hypothetical protein | - |
| P1J21_RS25900 (98976) | 98976..99260 | + | 285 | WP_000624109.1 | type-F conjugative transfer system pilin chaperone TraQ | - |
| P1J21_RS25905 (99247) | 99247..99792 | + | 546 | WP_000059851.1 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | - |
| P1J21_RS25910 (99722) | 99722..100063 | + | 342 | WP_001443031.1 | P-type conjugative transfer protein TrbJ | - |
| P1J21_RS25915 (100050) | 100050..100430 | + | 381 | WP_001389370.1 | F-type conjugal transfer protein TrbF | - |
| P1J21_RS25920 (100430) | 100430..101803 | + | 1374 | WP_001517790.1 | conjugal transfer pilus assembly protein TraH | - |
| P1J21_RS25925 (101800) | 101800..104622 | + | 2823 | WP_001007018.1 | conjugal transfer mating-pair stabilization protein TraG | - |
| P1J21_RS25930 (104638) | 104638..105135 | + | 498 | WP_000605857.1 | entry exclusion protein | - |
| P1J21_RS25935 (105167) | 105167..105898 | + | 732 | WP_023565183.1 | conjugal transfer complement resistance protein TraT | - |
| P1J21_RS25940 (106151) | 106151..107431 | + | 1281 | Protein_115 | type IV conjugative transfer system coupling protein TraD | - |
| P1J21_RS25945 (107451) | 107451..107678 | - | 228 | WP_000450520.1 | toxin-antitoxin system antitoxin VapB | - |
| P1J21_RS25950 (107760) | 107760..113030 | + | 5271 | WP_000986962.1 | Multifunctional conjugation protein TraI | Relaxase |
| P1J21_RS25955 (113050) | 113050..113796 | + | 747 | WP_276170845.1 | conjugal transfer pilus acetylase TraX | - |
| P1J21_RS25960 (113855) | 113855..114715 | + | 861 | WP_000704514.1 | Quorum-quenching protein AidA | VF |
| P1J21_RS25965 (114818) | 114818..115378 | + | 561 | WP_000139315.1 | fertility inhibition protein FinO | - |
| P1J21_RS25970 (115514) | 115514..115726 | + | 213 | WP_001299730.1 | ANR family transcriptional regulator | - |
| P1J21_RS25975 (115972) | 115972..116046 | + | 75 | Protein_122 | endonuclease | - |
| - (116302) | 116302..116363 | - | 62 | NuclAT_1 | - | Antitoxin Detail |
| - (116302) | 116302..116363 | - | 62 | NuclAT_1 | - | Antitoxin Detail |
| - (116302) | 116302..116363 | - | 62 | NuclAT_1 | - | Antitoxin Detail |
| - (116302) | 116302..116363 | - | 62 | NuclAT_1 | - | Antitoxin Detail |
| P1J21_RS25980 (116407) | 116407..116556 | + | 150 | WP_001312851.1 | Hok/Gef family protein | Toxin Detail |
| P1J21_RS25985 (116840) | 116840..117097 | + | 258 | WP_000083845.1 | replication regulatory protein RepA | - |
| P1J21_RS25990 (117114) | 117114..117365 | - | 252 | WP_223195197.1 | replication protein RepA | - |
| P1J21_RS25995 (117356) | 117356..117403 | + | 48 | WP_229471593.1 | hypothetical protein | - |
| P1J21_RS26000 (117396) | 117396..117878 | + | 483 | WP_001273588.1 | hypothetical protein | - |
| P1J21_RS26005 (117871) | 117871..118728 | + | 858 | WP_105075777.1 | incFII family plasmid replication initiator RepA | - |
| P1J21_RS26010 (119429) | 119429..119569 | + | 141 | WP_001333237.1 | hypothetical protein | - |
| P1J21_RS26015 (119667) | 119667..120059 | + | 393 | WP_000616804.1 | histidine phosphatase family protein | - |
| P1J21_RS26020 (120052) | 120052..120309 | + | 258 | WP_230133887.1 | CPBP family intramembrane metalloprotease | - |
| P1J21_RS26025 (120402) | 120402..120659 | + | 258 | WP_000557619.1 | type II toxin-antitoxin system antitoxin PemI | - |
| P1J21_RS26030 (120592) | 120592..120993 | + | 402 | WP_001398199.1 | type II toxin-antitoxin system toxin endoribonuclease PemK | - |
| P1J21_RS26035 (121242) | 121242..121939 | + | 698 | WP_095033700.1 | IS1-like element IS1B family transposase | - |
| P1J21_RS26040 (122046) | 122046..122480 | - | 435 | WP_000429836.1 | IS607 family transposase ISChh1 | Transposase |
| P1J21_RS26045 (122552) | 122552..122902 | + | 351 | WP_001294663.1 | mercuric transport protein MerT | - |
| P1J21_RS26050 (122916) | 122916..123191 | + | 276 | WP_000732292.1 | mercury resistance system periplasmic binding protein MerP | - |
| P1J21_RS26055 (123227) | 123227..123649 | + | 423 | WP_001340589.1 | organomercurial transporter MerC | - |
| P1J21_RS26060 (123701) | 123701..125395 | + | 1695 | WP_000105636.1 | mercury(II) reductase | - |
| P1J21_RS26065 (125413) | 125413..125775 | + | 363 | WP_001277456.1 | mercury resistance co-regulator MerD | - |
| P1J21_RS26070 (125772) | 125772..126008 | + | 237 | WP_000993386.1 | broad-spectrum mercury transporter MerE | - |
| P1J21_RS26075 (126044) | 126044..126712 | + | 669 | WP_001300294.1 | putative signaling protein | VF |
| P1J21_RS26080 (126787) | 126787..128037 | + | 1251 | Protein_143 | transposase | - |
| P1J21_RS26085 (128102) | 128102..128806 | + | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| P1J21_RS26090 (128908) | 128908..129387 | + | 480 | WP_000888203.1 | Rrf2 family transcriptional regulator | - |
Similar MGE(s)
Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE.
| Detail | Organism | MGE type | Related TA | Genome accession | Coordinates | Mash distance |
|---|