MGE detailed information
MGE type: Conjugative plasmid MGE length: 136642 bp
Accession: NZ_CP103743 Location: 1..136642 Organism: Escherichia coli O25b:H4-ST131 strain 2822 Replicon: plasmid pMB2855_1
Accession: NZ_CP103743 Location: 1..136642 Organism: Escherichia coli O25b:H4-ST131 strain 2822 Replicon: plasmid pMB2855_1
Gene structure
The 5 kb flanking regions of this MGE are displayed.
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| M5R22_RS23755 (1) | 1..756 | + | 756 | WP_000852146.1 | replication initiation protein RepE | - |
| M5R22_RS23760 (1344) | 1344..2510 | + | 1167 | WP_000772446.1 | hypothetical protein | VF |
| M5R22_RS23765 (2510) | 2510..3481 | + | 972 | WP_000817036.1 | ParB/RepB/Spo0J family plasmid partition protein | - |
| M5R22_RS23770 (4348) | 4348..5250 | + | 903 | WP_001348615.1 | DUF1281 domain-containing protein | - |
| M5R22_RS23775 (5247) | 5247..5558 | + | 312 | WP_032143366.1 | hypothetical protein | - |
| M5R22_RS23780 (5635) | 5635..6318 | + | 684 | WP_000086185.1 | DNA methylase | - |
| M5R22_RS23785 (6319) | 6319..6540 | + | 222 | WP_001104873.1 | hypothetical protein | - |
| M5R22_RS23790 (6470) | 6470..6988 | + | 519 | WP_250910048.1 | DUF1380 family protein | - |
| M5R22_RS23795 (7039) | 7039..7815 | + | 777 | WP_001753849.1 | hypothetical protein | - |
| M5R22_RS23800 (8232) | 8232..8657 | + | 426 | WP_001198928.1 | antirestriction protein | - |
| M5R22_RS23805 (8704) | 8704..9126 | + | 423 | WP_000271762.1 | DUF1380 family protein | - |
| M5R22_RS23810 (9123) | 9123..9314 | + | 192 | WP_001027493.1 | hypothetical protein | - |
| M5R22_RS23815 (9628) | 9628..11295 | - | 1668 | WP_052991863.1 | group II intron reverse transcriptase/maturase | - |
| M5R22_RS23820 (12024) | 12024..12197 | - | 174 | Protein_13 | hypothetical protein | - |
| M5R22_RS23825 (12267) | 12267..12473 | + | 207 | WP_000275859.1 | hypothetical protein | - |
| M5R22_RS23830 (12498) | 12498..12785 | + | 288 | WP_000107535.1 | hypothetical protein | - |
| M5R22_RS23835 (12904) | 12904..13725 | + | 822 | WP_001234469.1 | DUF932 domain-containing protein | - |
| M5R22_RS23840 (14022) | 14022..14624 | - | 603 | WP_000243712.1 | hypothetical protein | VF |
| M5R22_RS23845 (14955) | 14955..15338 | + | 384 | WP_001151566.1 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| M5R22_RS23850 (15472) | 15472..16149 | + | 678 | WP_001348626.1 | PAS domain-containing protein | - |
| M5R22_RS23855 (16237) | 16237..16464 | + | 228 | WP_001254388.1 | conjugal transfer relaxosome protein TraY | - |
| M5R22_RS23860 (16498) | 16498..16860 | + | 363 | WP_000338606.1 | type IV conjugative transfer system pilin TraA | - |
| M5R22_RS23865 (16865) | 16865..17176 | + | 312 | WP_000012107.1 | type IV conjugative transfer system protein TraL | - |
| M5R22_RS23870 (17198) | 17198..17764 | + | 567 | WP_000399791.1 | type IV conjugative transfer system protein TraE | - |
| M5R22_RS23875 (17751) | 17751..18479 | + | 729 | WP_001230808.1 | type-F conjugative transfer system secretin TraK | - |
| M5R22_RS23880 (18479) | 18479..19930 | + | 1452 | WP_000146687.1 | F-type conjugal transfer pilus assembly protein TraB | - |
| M5R22_RS23885 (19920) | 19920..20486 | + | 567 | WP_001617877.1 | conjugal transfer pilus-stabilizing protein TraP | - |
| M5R22_RS23890 (20473) | 20473..20784 | + | 312 | WP_001057272.1 | conjugal transfer protein TrbD | - |
| M5R22_RS23895 (20784) | 20784..21299 | + | 516 | WP_000809886.1 | type IV conjugative transfer system lipoprotein TraV | - |
| M5R22_RS23900 (21434) | 21434..21655 | + | 222 | WP_001278694.1 | conjugal transfer protein TraR | - |
| M5R22_RS23905 (21815) | 21815..24442 | + | 2628 | WP_001064258.1 | type IV secretion system protein TraC | - |
| M5R22_RS23910 (24439) | 24439..24825 | + | 387 | WP_000214084.1 | type-F conjugative transfer system protein TrbI | - |
| M5R22_RS23915 (24822) | 24822..25455 | + | 634 | Protein_32 | type-F conjugative transfer system protein TraW | - |
| M5R22_RS23920 (25452) | 25452..26444 | + | 993 | WP_000830190.1 | conjugal transfer pilus assembly protein TraU | - |
| M5R22_RS23925 (26474) | 26474..26779 | + | 306 | WP_000224413.1 | hypothetical protein | - |
| M5R22_RS23930 (26788) | 26788..26880 | + | 93 | Protein_35 | type-F conjugative transfer system pilin assembly protein TrbC | - |
| M5R22_RS23935 (26935) | 26935..27632 | + | 698 | WP_223216367.1 | IS1 family transposase IS1G | Transposase |
| M5R22_RS23940 (27680) | 27680..28000 | + | 321 | WP_001348757.1 | conjugal transfer protein TrbA | - |
| M5R22_RS23945 (28127) | 28127..28411 | + | 285 | WP_001617873.1 | type-F conjugative transfer system pilin chaperone TraQ | - |
| M5R22_RS23950 (28398) | 28398..28943 | + | 546 | WP_000059829.1 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | - |
| M5R22_RS23955 (28873) | 28873..29220 | + | 348 | WP_071571857.1 | P-type conjugative transfer protein TrbJ | - |
| M5R22_RS23960 (29201) | 29201..29593 | + | 393 | WP_000659962.1 | F-type conjugal transfer protein TrbF | - |
| M5R22_RS23965 (29580) | 29580..30953 | + | 1374 | WP_181713521.1 | conjugal transfer pilus assembly protein TraH | - |
| M5R22_RS23970 (30950) | 30950..33769 | + | 2820 | WP_001007062.1 | conjugal transfer mating-pair stabilization protein TraG | - |
| M5R22_RS23975 (33788) | 33788..34285 | + | 498 | WP_000605862.1 | entry exclusion protein | - |
| M5R22_RS23980 (34317) | 34317..35048 | + | 732 | WP_000850429.1 | conjugal transfer complement resistance protein TraT | - |
| M5R22_RS23985 (35251) | 35251..36030 | + | 780 | WP_000199914.1 | hypothetical protein | - |
| M5R22_RS23990 (36027) | 36027..38243 | + | 2217 | WP_023356304.1 | type IV conjugative transfer system coupling protein TraD | - |
| M5R22_RS23995 (38243) | 38243..43513 | + | 5271 | WP_001617867.1 | Multifunctional conjugation protein TraI | Relaxase |
| M5R22_RS24000 (43533) | 43533..44279 | + | 747 | WP_000205718.1 | conjugal transfer pilus acetylase TraX | - |
| M5R22_RS24005 (44334) | 44334..44894 | + | 561 | WP_000139341.1 | fertility inhibition protein FinO | - |
| M5R22_RS24010 (45025) | 45025..45237 | + | 213 | WP_013023861.1 | hypothetical protein | - |
| M5R22_RS24015 (45796) | 45796..46221 | + | 426 | WP_000422741.1 | IS66 family transposase ISEc23 | Transposase |
| M5R22_RS24020 (46218) | 46218..46568 | + | 351 | WP_000624722.1 | IS66 family transposase ISEc23 | Transposase |
| M5R22_RS24025 (46599) | 46599..48212 | + | 1614 | WP_000080195.1 | IS66 family transposase ISEc23 | Transposase |
| M5R22_RS24030 (48290) | 48290..48576 | + | 287 | Protein_55 | DUF2726 domain-containing protein | - |
| - (48543) | 48543..48603 | - | 61 | NuclAT_0 | - | Antitoxin Detail |
| - (48543) | 48543..48603 | - | 61 | NuclAT_0 | - | Antitoxin Detail |
| - (48543) | 48543..48603 | - | 61 | NuclAT_0 | - | Antitoxin Detail |
| - (48543) | 48543..48603 | - | 61 | NuclAT_0 | - | Antitoxin Detail |
| M5R22_RS24035 (48648) | 48648..48782 | + | 135 | WP_023144756.1 | Hok/Gef family protein | Toxin Detail |
| M5R22_RS24040 (49079) | 49079..49333 | + | 255 | WP_000083850.1 | replication regulatory protein RepA | - |
| M5R22_RS24045 (49439) | 49439..49570 | + | 132 | Protein_58 | protein CopA/IncA | - |
| M5R22_RS24050 (49570) | 49570..49644 | + | 75 | WP_031943482.1 | RepA leader peptide Tap | - |
| M5R22_RS24055 (49637) | 49637..50494 | + | 858 | WP_001617855.1 | incFII family plasmid replication initiator RepA | - |
| M5R22_RS24060 (51434) | 51434..52087 | + | 654 | WP_000616807.1 | CPBP family intramembrane metalloprotease | - |
| M5R22_RS24065 (52180) | 52180..52437 | + | 258 | WP_000557619.1 | type II toxin-antitoxin system antitoxin PemI | - |
| M5R22_RS24070 (52370) | 52370..52771 | + | 402 | WP_001398199.1 | type II toxin-antitoxin system toxin endoribonuclease PemK | - |
| M5R22_RS24075 (53020) | 53020..53435 | + | 416 | Protein_64 | IS1 family transposase | - |
| M5R22_RS24080 (53471) | 53471..54016 | - | 546 | Protein_65 | Tn3 family transposase TnpA_Tn3 | Transposase |
| M5R22_RS24085 (54082) | 54082..54786 | - | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| M5R22_RS24090 (55100) | 55100..55975 | + | 876 | WP_032277257.1 | Beta-lactamase CTX-M-97 | ARG |
| M5R22_RS24095 (56010) | 56010..56372 | + | 363 | Protein_68 | IS5 family transposase IS903B | Transposase |
| M5R22_RS24100 (56432) | 56432..57136 | + | 705 | WP_001067858.1 | IS6 family transposase IS15DI | Transposase |
| M5R22_RS24105 (57189) | 57189..57521 | + | 333 | Protein_70 | EamA family transporter | - |
| M5R22_RS24110 (57553) | 57553..58752 | - | 1200 | WP_000804064.1 | Tetracycline resistance protein, class C | ARG |
| M5R22_RS24115 (58858) | 58858..59484 | + | 627 | WP_031606906.1 | tetracycline resistance transcriptional repressor TetR(A) | - |
| M5R22_RS24120 (59516) | 59516..59752 | - | 237 | WP_042005022.1 | hypothetical protein | - |
| M5R22_RS24125 (59806) | 59806..60642 | - | 837 | WP_000480972.1 | hypothetical protein | ARG |
| M5R22_RS24130 (60642) | 60642..61445 | - | 804 | WP_001082319.1 | Aminoglycoside 3'-phosphotransferase | ARG |
| M5R22_RS24135 (61506) | 61506..62321 | - | 816 | WP_001043260.1 | Dihydropteroate synthase | ARG |
| M5R22_RS24140 (62629) | 62629..62805 | - | 177 | Protein_77 | replication protein C, IncQ-type | - |
| M5R22_RS24145 (62866) | 62866..63570 | + | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| M5R22_RS24150 (63692) | 63692..64597 | + | 906 | WP_000219391.1 | hypothetical protein | ARG |
| M5R22_RS24155 (64594) | 64594..65832 | + | 1239 | WP_000004159.1 | macrolide resistance MFS transporter Mrx(A) | - |
| M5R22_RS24160 (65832) | 65832..66416 | + | 585 | WP_001137892.1 | macrolide-binding transcriptional repressor MphR(A) | - |
| M5R22_RS24165 (66909) | 66909..67673 | - | 765 | WP_001389365.1 | IS6 family transposase IS6100 | Transposase |
| M5R22_RS24170 (67791) | 67791..67856 | - | 66 | Protein_83 | EAL domain-containing protein | - |
| M5R22_RS24175 (67900) | 67900..68205 | - | 306 | WP_000130000.1 | Transcriptional regulator | Relaxase |
| M5R22_RS24180 (68216) | 68216..69421 | - | 1206 | WP_000184001.1 | chromate efflux transporter | - |
| M5R22_RS24185 (69577) | 69577..69780 | - | 204 | WP_000376616.1 | hypothetical protein | - |
| M5R22_RS24190 (69908) | 69908..70747 | - | 840 | WP_000259031.1 | Dihydropteroate synthase | ARG |
| M5R22_RS24195 (70741) | 70741..71088 | - | 348 | WP_000679427.1 | Multidrug transporter EmrE | ARG |
| M5R22_RS24200 (71294) | 71294..72082 | - | 789 | WP_000503573.1 | Streptomycin 3''-adenylyltransferase | ARG |
| M5R22_RS24205 (72213) | 72213..72686 | - | 474 | WP_001389366.1 | Dihydrofolate reductase type 1 | ARG |
| M5R22_RS24210 (72844) | 72844..73593 | + | 750 | Protein_91 | IS91 family transposase ISAzo26 | Transposase |
| M5R22_RS24215 (73652) | 73652..74356 | + | 705 | WP_001067858.1 | IS6 family transposase IS15DI | Transposase |
| M5R22_RS24220 (74676) | 74676..74789 | + | 114 | Protein_93 | IS6 family transposase | - |
| M5R22_RS24225 (74927) | 74927..75097 | + | 171 | Protein_94 | DsbA family protein | - |
| M5R22_RS24230 (75087) | 75087..75593 | + | 507 | WP_000949452.1 | protein disulfide oxidoreductase | - |
| M5R22_RS24235 (75830) | 75830..76591 | + | 762 | WP_001225378.1 | Na(+)-translocating NADH-quinone reductase subunit C | - |
| M5R22_RS24240 (76938) | 76938..78824 | + | 1887 | WP_000118029.1 | FTR1 family protein | - |
| M5R22_RS24245 (78865) | 78865..79392 | + | 528 | WP_000178050.1 | iron transporter | - |
| M5R22_RS24250 (79496) | 79496..80875 | + | 1380 | WP_000119836.1 | Fe-S-containing protein | - |
| M5R22_RS24255 (80878) | 80878..82161 | + | 1284 | WP_000964653.1 | ABC transporter permease | - |
| M5R22_RS24260 (82151) | 82151..83281 | + | 1131 | WP_000729218.1 | ABC transporter permease | - |
| M5R22_RS24265 (83286) | 83286..83981 | + | 696 | WP_000117262.1 | Lipoprotein-releasing system ATP-binding protein LolD | VF |
| M5R22_RS24270 (83968) | 83968..84453 | + | 486 | WP_001267176.1 | TlpA disulfide reductase family protein | - |
| M5R22_RS24275 (84478) | 84478..84963 | + | 486 | WP_000874189.1 | hypothetical protein | - |
| M5R22_RS24280 (85085) | 85085..85718 | + | 634 | Protein_105 | histidine-type phosphatase | - |
| M5R22_RS24285 (85868) | 85868..86041 | - | 174 | Protein_106 | IS5 family transposase ISKpn26 | Transposase |
| M5R22_RS24290 (86042) | 86042..86356 | - | 315 | Protein_107 | IS5 family transposase ISKpn26 | Transposase |
| M5R22_RS24295 (87029) | 87029..87768 | - | 740 | Protein_108 | IS3 family transposase IS629 | Transposase |
| M5R22_RS24300 (87870) | 87870..88130 | + | 261 | WP_000371882.1 | hypothetical protein | - |
| M5R22_RS24305 (88127) | 88127..88636 | + | 510 | WP_000194542.1 | hypothetical protein | - |
| M5R22_RS24310 (88656) | 88656..89003 | - | 348 | WP_000142452.1 | DUF6404 family protein | - |
| M5R22_RS24315 (89132) | 89132..89467 | + | 336 | WP_001080732.1 | colicin E1 family microcin immunity protein | - |
| M5R22_RS24320 (90919) | 90919..91544 | + | 626 | Protein_113 | replication initiation protein | - |
| M5R22_RS24325 (92123) | 92123..92827 | - | 705 | WP_001067858.1 | IS6 family transposase IS15DI | Transposase |
| M5R22_RS24330 (92818) | 92818..93621 | + | 804 | WP_259429303.1 | ChaN family lipoprotein | - |
| M5R22_RS24335 (93629) | 93629..94405 | + | 777 | WP_000981091.1 | energy transducer TonB | - |
| M5R22_RS24340 (94574) | 94574..96835 | + | 2262 | WP_001100763.1 | TonB-dependent receptor | - |
| M5R22_RS24345 (96904) | 96904..98079 | + | 1176 | WP_001020413.1 | putative protein YncE | VF |
| M5R22_RS24350 (98602) | 98602..98907 | - | 306 | Protein_119 | IS91 family transposase ISSbo1 | Transposase |
| M5R22_RS24355 (98933) | 98933..99672 | + | 740 | Protein_120 | IS3 family transposase IS629 | Transposase |
| M5R22_RS24360 (100345) | 100345..101853 | + | 1509 | WP_001189113.1 | group II intron reverse transcriptase/maturase | - |
| M5R22_RS24365 (101937) | 101937..102275 | + | 339 | Protein_122 | IS3 family transposase IS629 | Transposase |
| M5R22_RS24370 (102355) | 102355..102759 | + | 405 | WP_000839179.1 | IS66 family transposase ISEc22 | Transposase |
| M5R22_RS24375 (102756) | 102756..103103 | + | 348 | WP_000612626.1 | IS66 family transposase ISKpn24 | Transposase |
| M5R22_RS24380 (103152) | 103152..104097 | + | 946 | Protein_125 | IS66 family transposase ISEc8 | Transposase |
| M5R22_RS24385 (104183) | 104183..105205 | + | 1023 | WP_001310017.1 | IS21 family transposase IS100kyp | Transposase |
| M5R22_RS24390 (105202) | 105202..105984 | + | 783 | WP_001300609.1 | IS21 family transposase IS100kyp | Transposase |
| M5R22_RS24395 (106052) | 106052..106660 | + | 609 | Protein_128 | IS66 family transposase ISEc22 | Transposase |
| M5R22_RS24400 (106723) | 106723..107514 | - | 792 | WP_000977394.1 | DUF4198 domain-containing protein | - |
| M5R22_RS24405 (107521) | 107521..109491 | - | 1971 | WP_001298664.1 | Hemin receptor | VF |
| M5R22_RS24410 (109773) | 109773..110197 | + | 425 | Protein_131 | IS3 family transposase IS2 | Transposase |
| M5R22_RS24415 (110734) | 110734..111006 | + | 273 | WP_001322642.1 | adhesin biosynthesis transcription regulatory family protein | - |
| M5R22_RS24420 (111452) | 111452..111627 | + | 176 | Protein_133 | IS3 family transposase | - |
| M5R22_RS24425 (111853) | 111853..113022 | - | 1170 | WP_001072358.1 | hypothetical protein | - |
| M5R22_RS24430 (113389) | 113389..113577 | + | 189 | WP_001309252.1 | hypothetical protein | - |
| M5R22_RS24435 (113698) | 113698..114438 | + | 741 | WP_001066941.1 | tyrosine-type recombinase/integrase | - |
| M5R22_RS24440 (114723) | 114723..115700 | - | 978 | WP_000361610.1 | RepB family plasmid replication initiator protein | - |
| M5R22_RS24445 (116456) | 116456..117427 | + | 972 | Protein_138 | hypothetical protein | - |
| M5R22_RS24450 (117586) | 117586..117744 | - | 159 | WP_228955855.1 | abortive infection family protein | - |
| M5R22_RS24455 (117892) | 117892..118589 | + | 698 | WP_223293341.1 | IS1 family transposase IS1R | Transposase |
| M5R22_RS24460 (118605) | 118605..118934 | + | 330 | Protein_141 | TOBE domain-containing protein | - |
| M5R22_RS24465 (119043) | 119043..119975 | - | 933 | WP_000081352.1 | homocysteine S-methyltransferase | - |
| M5R22_RS24470 (119962) | 119962..121365 | - | 1404 | WP_001373486.1 | S-methylmethionine permease | - |
| M5R22_RS24475 (121609) | 121609..122589 | + | 981 | WP_000019407.1 | IS5 family transposase IS5D | Transposase |
| M5R22_RS24480 (122799) | 122799..123134 | + | 336 | WP_169329198.1 | type I deoxyribonuclease HsdR | - |
| M5R22_RS24485 (123084) | 123084..123497 | - | 414 | Protein_146 | IS3 family transposase ISLad1 | Transposase |
| M5R22_RS24490 (123502) | 123502..123780 | - | 279 | Protein_147 | pdcB | - |
| M5R22_RS24495 (123808) | 123808..123987 | - | 180 | WP_001513661.1 | hypothetical protein | - |
| M5R22_RS24500 (123992) | 123992..124372 | - | 381 | WP_001216034.1 | type II toxin-antitoxin system death-on-curing family toxin | Toxin Detail |
| M5R22_RS24505 (124372) | 124372..124593 | - | 222 | WP_001190712.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin Detail |
| M5R22_RS24510 (124776) | 124776..126332 | + | 1557 | WP_001617892.1 | type I restriction-modification system subunit M | - |
| M5R22_RS24515 (126329) | 126329..127612 | + | 1284 | WP_001617890.1 | restriction endonuclease subunit S | - |
| M5R22_RS24520 (127734) | 127734..130850 | + | 3117 | WP_001553854.1 | HsdR family type I site-specific deoxyribonuclease | - |
| M5R22_RS24525 (131064) | 131064..131761 | + | 698 | WP_103215986.1 | IS1 family transposase IS1G | Transposase |
| M5R22_RS24530 (131898) | 131898..132791 | - | 894 | WP_001553853.1 | S-4TM family putative pore-forming effector | - |
| M5R22_RS24535 (132834) | 132834..133829 | - | 996 | WP_000246636.1 | hypothetical protein | - |
| M5R22_RS24540 (134537) | 134537..134755 | + | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| M5R22_RS24545 (134757) | 134757..135062 | + | 306 | WP_001159868.1 | type II toxin-antitoxin system toxin CcdB | - |
| M5R22_RS24550 (135063) | 135063..135869 | + | 807 | WP_000016982.1 | site-specific integrase | - |
Similar MGE(s)
Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE.
| Detail | Organism | MGE type | Related TA | Genome accession | Coordinates | Mash distance |
|---|