MGE detailed information
MGE type: Conjugative plasmid MGE length: 81007 bp
Accession: NZ_CP103712 Location: 1..81007 Organism: Escherichia coli O25b:H4-ST131 Replicon: plasmid pMB3266_2
Accession: NZ_CP103712 Location: 1..81007 Organism: Escherichia coli O25b:H4-ST131 Replicon: plasmid pMB3266_2
Gene structure
The 5 kb flanking regions of this MGE are displayed.
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
M5S69_RS25215 (1) | 1..756 | + | 756 | WP_000852146.1 | replication initiation protein RepE | - |
M5S69_RS25220 (1344) | 1344..2510 | + | 1167 | WP_000772446.1 | hypothetical protein | VF |
M5S69_RS25225 (2510) | 2510..3481 | + | 972 | WP_000817036.1 | ParB/RepB/Spo0J family plasmid partition protein | - |
M5S69_RS25230 (4305) | 4305..5207 | + | 903 | WP_001348615.1 | DUF1281 domain-containing protein | - |
M5S69_RS25235 (5204) | 5204..5515 | + | 312 | WP_032143366.1 | hypothetical protein | - |
M5S69_RS25240 (5592) | 5592..6275 | + | 684 | WP_000086185.1 | DNA methylase | - |
M5S69_RS25245 (6276) | 6276..6497 | + | 222 | WP_001104873.1 | hypothetical protein | - |
M5S69_RS25250 (6427) | 6427..6945 | + | 519 | WP_123325260.1 | DUF1380 family protein | - |
M5S69_RS25255 (6996) | 6996..7772 | + | 777 | WP_001753849.1 | hypothetical protein | - |
M5S69_RS25260 (8189) | 8189..8614 | + | 426 | WP_001198928.1 | antirestriction protein | - |
M5S69_RS25265 (8661) | 8661..9083 | + | 423 | WP_000271753.1 | DUF1380 family protein | - |
M5S69_RS25270 (9080) | 9080..9271 | + | 192 | WP_001027516.1 | hypothetical protein | - |
M5S69_RS25275 (10306) | 10306..10536 | + | 231 | WP_000218642.1 | hypothetical protein | - |
M5S69_RS25280 (10588) | 10588..11949 | + | 1362 | WP_000170714.1 | DUF3560 domain-containing protein | - |
M5S69_RS25285 (11997) | 11997..12560 | + | 564 | WP_001348621.1 | class I SAM-dependent methyltransferase | - |
M5S69_RS25290 (12586) | 12586..12858 | + | 273 | WP_048264352.1 | hypothetical protein | - |
M5S69_RS25295 (13021) | 13021..13356 | + | 336 | WP_013023883.1 | hypothetical protein | - |
M5S69_RS25300 (13269) | 13269..13475 | + | 207 | WP_000547975.1 | hypothetical protein | - |
M5S69_RS25305 (13501) | 13501..14040 | + | 540 | WP_000290841.1 | single-stranded DNA-binding protein | - |
M5S69_RS25310 (14103) | 14103..14336 | + | 234 | WP_000005990.1 | DUF905 family protein | - |
M5S69_RS25315 (14402) | 14402..16360 | + | 1959 | WP_000117179.1 | ParB/RepB/Spo0J family partition protein | - |
M5S69_RS25320 (16415) | 16415..16849 | + | 435 | WP_000845940.1 | conjugation system SOS inhibitor PsiB | - |
M5S69_RS25325 (16846) | 16846..17608 | + | 763 | Protein_22 | plasmid SOS inhibition protein A | - |
- (17577) | 17577..17763 | + | 187 | NuclAT_0 | - | - |
- (17577) | 17577..17763 | + | 187 | NuclAT_0 | - | - |
- (17577) | 17577..17763 | + | 187 | NuclAT_0 | - | - |
- (17577) | 17577..17763 | + | 187 | NuclAT_0 | - | - |
M5S69_RS25330 (17811) | 17811..19180 | + | 1370 | WP_085947770.1 | IS3-like element IS150 family transposase | - |
- (19207) | 19207..19247 | + | 41 | NuclAT_1 | - | Antitoxin Detail |
- (19207) | 19207..19247 | + | 41 | NuclAT_1 | - | Antitoxin Detail |
- (19207) | 19207..19247 | + | 41 | NuclAT_1 | - | Antitoxin Detail |
- (19207) | 19207..19247 | + | 41 | NuclAT_1 | - | Antitoxin Detail |
M5S69_RS25335 (19233) | 19233..19382 | + | 150 | Protein_24 | plasmid maintenance protein Mok | - |
M5S69_RS25340 (19324) | 19324..19449 | + | 126 | WP_001372321.1 | type I toxin-antitoxin system Hok family toxin | Toxin Detail |
M5S69_RS25345 (19669) | 19669..19899 | + | 231 | WP_001426396.1 | hypothetical protein | - |
M5S69_RS25350 (19897) | 19897..20070 | - | 174 | Protein_27 | hypothetical protein | - |
M5S69_RS25355 (20140) | 20140..20346 | + | 207 | WP_000275859.1 | hypothetical protein | - |
M5S69_RS25360 (20371) | 20371..20658 | + | 288 | WP_000107535.1 | hypothetical protein | - |
M5S69_RS25365 (20777) | 20777..21598 | + | 822 | WP_001234469.1 | DUF932 domain-containing protein | - |
M5S69_RS25370 (21895) | 21895..22497 | - | 603 | WP_000243712.1 | hypothetical protein | VF |
M5S69_RS25375 (22828) | 22828..23211 | + | 384 | WP_001151562.1 | conjugal transfer relaxosome DNA-binding protein TraM | - |
M5S69_RS25380 (23405) | 23405..24091 | + | 687 | WP_000332487.1 | PAS domain-containing protein | - |
M5S69_RS25385 (24185) | 24185..24412 | + | 228 | WP_001254388.1 | conjugal transfer relaxosome protein TraY | - |
M5S69_RS25390 (24446) | 24446..24805 | + | 360 | WP_001098988.1 | type IV conjugative transfer system pilin TraA | - |
M5S69_RS25395 (24820) | 24820..25131 | + | 312 | WP_000012100.1 | type IV conjugative transfer system protein TraL | - |
M5S69_RS25400 (25153) | 25153..25719 | + | 567 | WP_000399776.1 | type IV conjugative transfer system protein TraE | - |
M5S69_RS25405 (25706) | 25706..26434 | + | 729 | WP_259420075.1 | type-F conjugative transfer system secretin TraK | - |
M5S69_RS25410 (26434) | 26434..27861 | + | 1428 | WP_000146665.1 | F-type conjugal transfer pilus assembly protein TraB | - |
M5S69_RS25415 (27851) | 27851..28441 | + | 591 | WP_000002787.1 | conjugal transfer pilus-stabilizing protein TraP | - |
M5S69_RS25420 (28428) | 28428..28748 | + | 321 | WP_001057274.1 | conjugal transfer protein TrbD | - |
M5S69_RS25425 (28741) | 28741..28993 | + | 253 | Protein_42 | conjugal transfer protein TrbG | - |
M5S69_RS25430 (28990) | 28990..29505 | + | 516 | WP_000809889.1 | type IV conjugative transfer system lipoprotein TraV | - |
M5S69_RS25435 (29640) | 29640..29861 | + | 222 | WP_001278694.1 | conjugal transfer protein TraR | - |
M5S69_RS25440 (29854) | 29854..30327 | + | 474 | WP_000549566.1 | hypothetical protein | - |
M5S69_RS25445 (30407) | 30407..30625 | + | 219 | WP_000556746.1 | hypothetical protein | - |
M5S69_RS25450 (30992) | 30992..33622 | + | 2631 | WP_000069778.1 | type IV secretion system protein TraC | - |
M5S69_RS25455 (33619) | 33619..34005 | + | 387 | WP_000214082.1 | type-F conjugative transfer system protein TrbI | - |
M5S69_RS25460 (34002) | 34002..34634 | + | 633 | WP_001203736.1 | type-F conjugative transfer system protein TraW | - |
M5S69_RS25465 (34631) | 34631..35623 | + | 993 | WP_000830838.1 | conjugal transfer pilus assembly protein TraU | - |
M5S69_RS25470 (35653) | 35653..35958 | + | 306 | WP_000224418.1 | hypothetical protein | - |
M5S69_RS25475 (35967) | 35967..36605 | + | 639 | WP_001080251.1 | type-F conjugative transfer system pilin assembly protein TrbC | - |
M5S69_RS25480 (36602) | 36602..38452 | + | 1851 | WP_000821859.1 | type-F conjugative transfer system mating-pair stabilization protein TraN | - |
M5S69_RS25485 (38479) | 38479..38736 | + | 258 | WP_000864314.1 | conjugal transfer protein TrbE | - |
M5S69_RS25490 (38729) | 38729..39472 | + | 744 | WP_001030385.1 | type-F conjugative transfer system pilin assembly protein TraF | - |
M5S69_RS25495 (39486) | 39486..39827 | + | 342 | WP_001298557.1 | conjugal transfer protein TrbA | - |
M5S69_RS25500 (39808) | 39808..40143 | - | 336 | WP_000736414.1 | hypothetical protein | - |
M5S69_RS25505 (40224) | 40224..40508 | + | 285 | WP_000624107.1 | type-F conjugative transfer system pilin chaperone TraQ | - |
M5S69_RS25510 (40495) | 40495..41040 | + | 546 | WP_000059830.1 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | - |
M5S69_RS25515 (40970) | 40970..41317 | + | 348 | WP_001309242.1 | P-type conjugative transfer protein TrbJ | - |
M5S69_RS25520 (41298) | 41298..41690 | + | 393 | WP_000660700.1 | F-type conjugal transfer protein TrbF | - |
M5S69_RS25525 (41677) | 41677..43050 | + | 1374 | WP_000944331.1 | conjugal transfer pilus assembly protein TraH | - |
M5S69_RS25530 (43047) | 43047..45869 | + | 2823 | WP_001007080.1 | conjugal transfer mating-pair stabilization protein TraG | - |
M5S69_RS25535 (45885) | 45885..46370 | + | 486 | WP_000605869.1 | hypothetical protein | - |
M5S69_RS25540 (46419) | 46419..47150 | + | 732 | WP_000782451.1 | conjugal transfer complement resistance protein TraT | - |
M5S69_RS25545 (47353) | 47353..48090 | + | 738 | WP_000199901.1 | hypothetical protein | - |
M5S69_RS25550 (48141) | 48141..50285 | + | 2145 | WP_000009347.1 | type IV conjugative transfer system coupling protein TraD | - |
M5S69_RS25555 (50285) | 50285..55555 | + | 5271 | WP_000948349.1 | Multifunctional conjugation protein TraI | Relaxase |
M5S69_RS25560 (55575) | 55575..56321 | + | 747 | WP_019842127.1 | conjugal transfer pilus acetylase TraX | - |
M5S69_RS25565 (56376) | 56376..56933 | + | 558 | WP_000139329.1 | fertility inhibition protein FinO | - |
M5S69_RS25570 (57085) | 57085..57288 | + | 204 | WP_001336517.1 | hypothetical protein | - |
M5S69_RS25575 (58030) | 58030..58491 | + | 462 | WP_019842128.1 | thermonuclease family protein | - |
M5S69_RS25580 (58760) | 58760..58978 | + | 219 | WP_023150739.1 | hypothetical protein | - |
M5S69_RS25585 (59131) | 59131..59568 | + | 438 | WP_000872609.1 | hypothetical protein | - |
- (59663) | 59663..59719 | - | 57 | NuclAT_2 | - | Antitoxin Detail |
- (59663) | 59663..59719 | - | 57 | NuclAT_2 | - | Antitoxin Detail |
- (59663) | 59663..59719 | - | 57 | NuclAT_2 | - | Antitoxin Detail |
- (59663) | 59663..59719 | - | 57 | NuclAT_2 | - | Antitoxin Detail |
M5S69_RS25590 (59767) | 59767..59916 | + | 150 | WP_001336447.1 | Hok/Gef family protein | Toxin Detail |
M5S69_RS25595 (60201) | 60201..60458 | + | 258 | WP_000084404.1 | replication regulatory protein RepA | - |
M5S69_RS25600 (60694) | 60694..60768 | + | 75 | WP_001365705.1 | RepA leader peptide Tap | - |
M5S69_RS25605 (60761) | 60761..61618 | + | 858 | WP_000130646.1 | incFII family plasmid replication initiator RepA | - |
M5S69_RS25610 (62531) | 62531..62668 | + | 138 | Protein_79 | XRE family transcriptional regulator | - |
M5S69_RS25615 (62688) | 62688..62951 | + | 264 | WP_001089473.1 | hypothetical protein | - |
M5S69_RS25620 (62941) | 62941..63240 | + | 300 | WP_001528606.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
M5S69_RS25625 (63276) | 63276..63941 | - | 666 | WP_001535733.1 | hypothetical protein | - |
M5S69_RS25630 (64129) | 64129..64989 | - | 861 | WP_000027057.1 | Beta-lactamase TEM | ARG |
M5S69_RS25635 (65121) | 65121..65825 | + | 705 | WP_001067858.1 | IS6 family transposase IS15DI | Transposase |
M5S69_RS25640 (65974) | 65974..66954 | + | 981 | WP_000019407.1 | IS5 family transposase IS5D | Transposase |
M5S69_RS25645 (67164) | 67164..67499 | + | 336 | WP_169329198.1 | type I deoxyribonuclease HsdR | - |
M5S69_RS25650 (67449) | 67449..67862 | - | 414 | Protein_87 | IS3 family transposase ISLad1 | Transposase |
M5S69_RS25655 (67867) | 67867..68145 | - | 279 | Protein_88 | pdcB | - |
M5S69_RS25660 (68173) | 68173..68352 | - | 180 | WP_001513661.1 | hypothetical protein | - |
M5S69_RS25665 (68357) | 68357..68737 | - | 381 | WP_001216034.1 | type II toxin-antitoxin system death-on-curing family toxin | Toxin Detail |
M5S69_RS25670 (68737) | 68737..68958 | - | 222 | WP_001190712.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin Detail |
M5S69_RS25675 (69141) | 69141..70697 | + | 1557 | WP_001617892.1 | type I restriction-modification system subunit M | - |
M5S69_RS25680 (70694) | 70694..71977 | + | 1284 | WP_001617890.1 | restriction endonuclease subunit S | - |
M5S69_RS25685 (72099) | 72099..75215 | + | 3117 | WP_001553854.1 | HsdR family type I site-specific deoxyribonuclease | - |
M5S69_RS25690 (75429) | 75429..76126 | + | 698 | WP_103215986.1 | IS1 family transposase IS1R | Transposase |
M5S69_RS25695 (76263) | 76263..77156 | - | 894 | WP_001553853.1 | S-4TM family putative pore-forming effector | - |
M5S69_RS25700 (77199) | 77199..78194 | - | 996 | WP_000246636.1 | hypothetical protein | - |
M5S69_RS25705 (78902) | 78902..79120 | + | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | - |
M5S69_RS25710 (79122) | 79122..79427 | + | 306 | WP_001159868.1 | type II toxin-antitoxin system toxin CcdB | - |
M5S69_RS25715 (79428) | 79428..80234 | + | 807 | WP_000016982.1 | site-specific integrase | - |
Similar MGE(s)
Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE.
Detail | Organism | MGE type | Related TA | Genome accession | Coordinates | Mash distance |
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