256302

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 68357..68958 Replicon plasmid pMB3266_2
Accession NZ_CP103712
Organism Escherichia coli O25b:H4-ST131

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag M5S69_RS25665 Protein ID WP_001216034.1
Coordinates 68357..68737 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag M5S69_RS25670 Protein ID WP_001190712.1
Coordinates 68737..68958 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S69_RS25630 (64129) 64129..64989 - 861 WP_000027057.1 broad-spectrum class A beta-lactamase TEM-1 -
M5S69_RS25635 (65121) 65121..65825 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
M5S69_RS25640 (65974) 65974..66954 + 981 WP_000019407.1 IS5-like element IS5 family transposase -
M5S69_RS25645 (67164) 67164..67499 + 336 WP_169329198.1 type I deoxyribonuclease HsdR -
M5S69_RS25650 (67449) 67449..67862 - 414 Protein_87 integrase core domain-containing protein -
M5S69_RS25655 (67867) 67867..68145 - 279 Protein_88 pdcB -
M5S69_RS25660 (68173) 68173..68352 - 180 WP_001513661.1 hypothetical protein -
M5S69_RS25665 (68357) 68357..68737 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
M5S69_RS25670 (68737) 68737..68958 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
M5S69_RS25675 (69141) 69141..70697 + 1557 WP_001617892.1 type I restriction-modification system subunit M -
M5S69_RS25680 (70694) 70694..71977 + 1284 WP_001617890.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B - 1..81007 81007


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T256302 WP_001216034.1 NZ_CP103712:c68737-68357 [Escherichia coli O25b:H4-ST131]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT256302 WP_001190712.1 NZ_CP103712:c68958-68737 [Escherichia coli O25b:H4-ST131]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References