MGE detailed information
MGE type: Conjugative plasmid MGE length: 133836 bp
Accession: NZ_CP103530 Location: 1..133836 Organism: Escherichia coli strain 5270 Replicon: plasmid pMB9245_1
Accession: NZ_CP103530 Location: 1..133836 Organism: Escherichia coli strain 5270 Replicon: plasmid pMB9245_1
Gene structure
The 5 kb flanking regions of this MGE are displayed.
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| M5T24_RS24135 (1) | 1..756 | + | 756 | WP_000852146.1 | replication initiation protein RepE | - |
| M5T24_RS24140 (1344) | 1344..2510 | + | 1167 | WP_000772446.1 | hypothetical protein | VF |
| M5T24_RS24145 (2510) | 2510..3481 | + | 972 | WP_000817031.1 | ParB/RepB/Spo0J family plasmid partition protein | - |
| M5T24_RS24150 (4219) | 4219..5121 | + | 903 | WP_064766247.1 | DUF1281 domain-containing protein | - |
| M5T24_RS24155 (5118) | 5118..5429 | + | 312 | WP_012615291.1 | hypothetical protein | - |
| M5T24_RS24160 (5506) | 5506..6189 | + | 684 | WP_032181561.1 | DNA methylase | - |
| M5T24_RS24165 (6190) | 6190..6411 | + | 222 | WP_001104873.1 | hypothetical protein | - |
| M5T24_RS24170 (6341) | 6341..6859 | + | 519 | WP_223349485.1 | DUF1380 family protein | - |
| M5T24_RS24175 (6904) | 6904..7674 | + | 771 | WP_001006251.1 | hypothetical protein | - |
| M5T24_RS24180 (8088) | 8088..8513 | + | 426 | WP_001546490.1 | antirestriction protein | - |
| M5T24_RS24185 (8560) | 8560..8982 | + | 423 | WP_000271685.1 | DUF1380 family protein | - |
| M5T24_RS24190 (8979) | 8979..9170 | + | 192 | WP_001027495.1 | hypothetical protein | - |
| M5T24_RS24195 (9483) | 9483..11150 | - | 1668 | WP_012372796.1 | group II intron reverse transcriptase/maturase | - |
| M5T24_RS24200 (12812) | 12812..13381 | - | 570 | WP_089634947.1 | hypothetical protein | - |
| M5T24_RS24210 (14685) | 14685..15443 | + | 759 | Protein_15 | site-specific integrase | - |
| M5T24_RS24215 (15601) | 15601..16611 | + | 1011 | WP_000053332.1 | substrate-binding domain-containing protein | - |
| M5T24_RS24220 (16707) | 16707..18833 | + | 2127 | WP_000253905.1 | alpha-galactosidase | - |
| M5T24_RS24225 (18896) | 18896..20173 | + | 1278 | WP_001083369.1 | MFS transporter | - |
| M5T24_RS24230 (20170) | 20170..21603 | + | 1434 | WP_024170639.1 | glycoside hydrolase family 32 protein | - |
| M5T24_RS24235 (21798) | 21798..23192 | + | 1395 | WP_001037799.1 | carbohydrate porin | - |
| M5T24_RS24240 (23438) | 23438..23542 | - | 105 | Protein_21 | thiosulfate reductase cytochrome B subunit | - |
| M5T24_RS24245 (23599) | 23599..24292 | + | 694 | Protein_22 | IS1 family transposase IS1A | Transposase |
| M5T24_RS24250 (24419) | 24419..24931 | + | 513 | WP_223350513.1 | potassium channel family protein | - |
| M5T24_RS24255 (25016) | 25016..25267 | + | 252 | Protein_24 | iron-containing alcohol dehydrogenase | - |
| M5T24_RS24260 (25556) | 25556..25873 | - | 318 | Protein_25 | IS110 family transposase | - |
| M5T24_RS24265 (26028) | 26028..26133 | + | 106 | Protein_26 | plasmid SOS inhibition protein A | - |
| - (26102) | 26102..26300 | + | 199 | NuclAT_0 | - | Antitoxin Detail |
| - (26102) | 26102..26300 | + | 199 | NuclAT_0 | - | Antitoxin Detail |
| - (26102) | 26102..26300 | + | 199 | NuclAT_0 | - | Antitoxin Detail |
| - (26102) | 26102..26300 | + | 199 | NuclAT_0 | - | Antitoxin Detail |
| M5T24_RS24270 (26307) | 26307..26456 | + | 150 | Protein_27 | DUF5431 family protein | - |
| M5T24_RS24275 (26407) | 26407..26523 | + | 117 | WP_001375150.1 | type I toxin-antitoxin system Hok family toxin | Toxin Detail |
| M5T24_RS24280 (26824) | 26824..27121 | - | 298 | Protein_29 | hypothetical protein | - |
| M5T24_RS24285 (27191) | 27191..27397 | + | 207 | WP_000275854.1 | hypothetical protein | - |
| M5T24_RS24290 (27422) | 27422..27709 | + | 288 | WP_000107242.1 | hypothetical protein | - |
| M5T24_RS24295 (27828) | 27828..28649 | + | 822 | WP_001234465.1 | DUF932 domain-containing protein | - |
| M5T24_RS24300 (28945) | 28945..29517 | - | 573 | WP_000949660.1 | hypothetical protein | VF |
| M5T24_RS24305 (29887) | 29887..30270 | + | 384 | WP_001063020.1 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| M5T24_RS24310 (30461) | 30461..31108 | + | 648 | WP_000332520.1 | conjugal transfer transcriptional regulator TraJ | - |
| M5T24_RS24315 (31244) | 31244..31459 | + | 216 | WP_001352842.1 | conjugal transfer relaxosome protein TraY | - |
| M5T24_RS24320 (31502) | 31502..31867 | + | 366 | WP_000340282.1 | type IV conjugative transfer system pilin TraA | - |
| M5T24_RS24325 (31882) | 31882..32193 | + | 312 | WP_000012106.1 | type IV conjugative transfer system protein TraL | - |
| M5T24_RS24330 (32215) | 32215..32781 | + | 567 | WP_000399790.1 | type IV conjugative transfer system protein TraE | - |
| M5T24_RS24335 (32768) | 32768..33496 | + | 729 | WP_001230813.1 | type-F conjugative transfer system secretin TraK | - |
| M5T24_RS24340 (33496) | 33496..34923 | + | 1428 | WP_001553836.1 | F-type conjugal transfer pilus assembly protein TraB | - |
| M5T24_RS24345 (34913) | 34913..35503 | + | 591 | WP_000002787.1 | conjugal transfer pilus-stabilizing protein TraP | - |
| M5T24_RS24350 (35490) | 35490..35810 | + | 321 | Protein_43 | conjugal transfer protein TrbD | - |
| M5T24_RS24355 (35803) | 35803..36054 | + | 252 | WP_001038342.1 | conjugal transfer protein TrbG | - |
| M5T24_RS24360 (36051) | 36051..36566 | + | 516 | WP_000809832.1 | type IV conjugative transfer system lipoprotein TraV | - |
| M5T24_RS24365 (36701) | 36701..36922 | + | 222 | WP_001278692.1 | conjugal transfer protein TraR | - |
| M5T24_RS24370 (37082) | 37082..39709 | + | 2628 | WP_039021964.1 | type IV secretion system protein TraC | - |
| M5T24_RS24375 (39706) | 39706..40092 | + | 387 | WP_000214092.1 | type-F conjugative transfer system protein TrbI | - |
| M5T24_RS24380 (40089) | 40089..40721 | + | 633 | WP_001203720.1 | type-F conjugative transfer system protein TraW | - |
| M5T24_RS24385 (40718) | 40718..41710 | + | 993 | WP_001553833.1 | conjugal transfer pilus assembly protein TraU | - |
| M5T24_RS24390 (41719) | 41719..42357 | + | 639 | WP_001553832.1 | type-F conjugative transfer system pilin assembly protein TrbC | - |
| M5T24_RS24395 (42354) | 42354..44162 | + | 1809 | WP_039023153.1 | type-F conjugative transfer system mating-pair stabilization protein TraN | - |
| M5T24_RS24400 (44189) | 44189..44446 | + | 258 | WP_039023152.1 | conjugal transfer protein TrbE | - |
| M5T24_RS24405 (44439) | 44439..45182 | + | 744 | WP_143212015.1 | type-F conjugative transfer system pilin assembly protein TraF | - |
| M5T24_RS24410 (45198) | 45198..45545 | + | 348 | WP_001287894.1 | conjugal transfer protein TrbA | - |
| M5T24_RS24415 (45664) | 45664..45948 | + | 285 | WP_000624110.1 | type-F conjugative transfer system pilin chaperone TraQ | - |
| M5T24_RS24420 (45935) | 45935..46480 | + | 546 | WP_016236310.1 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | - |
| M5T24_RS24425 (46410) | 46410..46772 | + | 363 | WP_071550055.1 | P-type conjugative transfer protein TrbJ | - |
| M5T24_RS24430 (46772) | 46772..48145 | + | 1374 | WP_039023150.1 | conjugal transfer pilus assembly protein TraH | - |
| M5T24_RS24435 (48142) | 48142..50961 | + | 2820 | WP_039023149.1 | conjugal transfer mating-pair stabilization protein TraG | - |
| M5T24_RS24440 (50980) | 50980..51477 | + | 498 | WP_000605862.1 | entry exclusion protein | - |
| M5T24_RS24445 (51509) | 51509..52240 | + | 732 | WP_000850422.1 | conjugal transfer complement resistance protein TraT | - |
| M5T24_RS24450 (52493) | 52493..54727 | + | 2235 | WP_062914766.1 | type IV conjugative transfer system coupling protein TraD | - |
| M5T24_RS24455 (54736) | 54736..55134 | - | 399 | WP_039023147.1 | type II toxin-antitoxin system VapC family toxin | Toxin Detail |
| M5T24_RS24460 (55134) | 55134..55361 | - | 228 | WP_039023146.1 | toxin-antitoxin system antitoxin VapB | Antitoxin Detail |
| M5T24_RS24465 (55443) | 55443..60713 | + | 5271 | WP_039023145.1 | Multifunctional conjugation protein TraI | Relaxase |
| M5T24_RS24470 (60989) | 60989..62530 | + | 1542 | WP_002431311.1 | IS21 family transposase ISEc12 | Transposase |
| M5T24_RS24475 (62545) | 62545..63291 | + | 747 | WP_001016257.1 | IS21 family transposase ISEc12 | Transposase |
| M5T24_RS24480 (63319) | 63319..64065 | + | 747 | WP_052273601.1 | conjugal transfer pilus acetylase TraX | - |
| M5T24_RS24485 (64120) | 64120..64680 | + | 561 | WP_001567328.1 | fertility inhibition protein FinO | - |
| M5T24_RS24490 (64811) | 64811..65020 | + | 210 | WP_039023209.1 | hypothetical protein | - |
| M5T24_RS24495 (65432) | 65432..66058 | + | 627 | WP_029702149.1 | NYN domain-containing protein | - |
| M5T24_RS24500 (66210) | 66210..66779 | + | 570 | WP_029702150.1 | DUF2726 domain-containing protein | - |
| M5T24_RS24505 (66992) | 66992..67420 | + | 429 | WP_029702151.1 | hypothetical protein | - |
| - (67559) | 67559..67620 | - | 62 | NuclAT_1 | - | - |
| - (67559) | 67559..67620 | - | 62 | NuclAT_1 | - | - |
| - (67559) | 67559..67620 | - | 62 | NuclAT_1 | - | - |
| - (67559) | 67559..67620 | - | 62 | NuclAT_1 | - | - |
| M5T24_RS24510 (67664) | 67664..67813 | + | 150 | WP_001367749.1 | Hok/Gef family protein | - |
| M5T24_RS24515 (68097) | 68097..68354 | + | 258 | WP_000083833.1 | replication regulatory protein RepA | - |
| M5T24_RS24520 (68590) | 68590..68664 | + | 75 | WP_001365705.1 | RepA leader peptide Tap | - |
| M5T24_RS24525 (68657) | 68657..69514 | + | 858 | WP_029702152.1 | incFII family plasmid replication initiator RepA | - |
| M5T24_RS24530 (70453) | 70453..71106 | + | 654 | WP_000616807.1 | CPBP family intramembrane metalloprotease | - |
| M5T24_RS24535 (71199) | 71199..71456 | + | 258 | WP_000557619.1 | type II toxin-antitoxin system antitoxin PemI | - |
| M5T24_RS24540 (71389) | 71389..71790 | + | 402 | WP_001398199.1 | type II toxin-antitoxin system toxin endoribonuclease PemK | - |
| M5T24_RS24545 (71927) | 71927..74824 | - | 2898 | WP_001553819.1 | Tn3 family transposase TnpA_Tn5403 | Transposase |
| M5T24_RS24550 (74919) | 74919..75524 | + | 606 | WP_000509965.1 | Tn3 family transposase TnpR_Tn5403 | Transposase |
| M5T24_RS24555 (75548) | 75548..76297 | - | 750 | Protein_84 | Tn3 family transposase TnpA_TnAs1 | Transposase |
| M5T24_RS24560 (76301) | 76301..76693 | + | 393 | WP_001351729.1 | isochorismatase family cysteine hydrolase | - |
| M5T24_RS24565 (76831) | 76831..77715 | + | 885 | WP_000058717.1 | EamA family transporter | - |
| M5T24_RS24570 (77747) | 77747..78946 | - | 1200 | WP_000804064.1 | Tetracycline resistance protein, class C | ARG |
| M5T24_RS24575 (79052) | 79052..79702 | + | 651 | WP_000164043.1 | tetracycline resistance transcriptional repressor TetR(A) | - |
| M5T24_RS24580 (79734) | 79734..79976 | - | 243 | WP_032140899.1 | hypothetical protein | - |
| M5T24_RS24585 (80018) | 80018..81532 | - | 1515 | Protein_90 | Tn3 family transposase TnpA_TnAs1 | Transposase |
| M5T24_RS24590 (81598) | 81598..82302 | + | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| M5T24_RS24595 (82446) | 82446..83000 | + | 555 | WP_063840321.1 | Aminoglycoside N(6')-acetyltransferase type 1 | ARG |
| M5T24_RS24600 (83131) | 83131..83961 | + | 831 | WP_001334766.1 | Beta-lactamase OXA-1 | ARG |
| M5T24_RS24605 (84099) | 84099..84539 | + | 441 | Protein_94 | CatB-related O-acetyltransferase | - |
| M5T24_RS24610 (84593) | 84593..85297 | - | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| M5T24_RS24615 (85363) | 85363..85650 | + | 288 | Protein_96 | transposase | - |
| M5T24_RS24620 (85906) | 85906..86781 | + | 876 | WP_000239590.1 | Beta-lactamase CTX-M-1 | ARG |
| M5T24_RS24625 (86828) | 86828..87121 | - | 294 | WP_116961751.1 | WbuC family cupin fold metalloprotein | - |
| M5T24_RS24630 (87186) | 87186..89405 | + | 2220 | Protein_99 | Tn3 family transposase TnpA_Tn3 | Transposase |
| M5T24_RS24635 (89482) | 89482..90186 | + | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| M5T24_RS24640 (90241) | 90241..91107 | - | 867 | Protein_101 | IS91 family transposase ISAzo26 | Transposase |
| M5T24_RS24645 (91252) | 91252..91749 | + | 498 | WP_001083725.1 | IS1595 family transposase ISSsu9 | ARG |
| M5T24_RS24650 (91861) | 91861..92151 | + | 291 | WP_001336345.1 | DUF1010 domain-containing protein | - |
| M5T24_RS24655 (92157) | 92157..92995 | + | 839 | Protein_104 | Streptomycin 3''-adenylyltransferase | ARG |
| M5T24_RS24660 (93112) | 93112..93459 | + | 348 | WP_000679427.1 | Multidrug transporter EmrE | ARG |
| M5T24_RS24665 (93453) | 93453..94292 | + | 840 | WP_000259031.1 | Dihydropteroate synthase | ARG |
| M5T24_RS24670 (94697) | 94697..96238 | + | 1542 | WP_000050481.1 | IS91 family transposase ISVsa3 | Transposase |
| M5T24_RS24675 (96551) | 96551..96925 | + | 375 | Protein_108 | protein-disulfide reductase DsbD family protein | - |
| M5T24_RS24680 (97025) | 97025..97582 | + | 558 | Protein_109 | twin-arginine translocation (TAT) pathway signal sequence domain protein | - |
| M5T24_RS24685 (97593) | 97593..98231 | - | 639 | WP_004201168.1 | phosphoribosylanthranilate isomerase | - |
| M5T24_RS24690 (98236) | 98236..98601 | - | 366 | WP_004201167.1 | bleomycin binding protein Ble-MBL | - |
| M5T24_RS24695 (98605) | 98605..99417 | - | 813 | WP_023408309.1 | Metallo-beta-lactamase type 2 | ARG |
| M5T24_RS24700 (99518) | 99518..99811 | - | 294 | Protein_113 | IS30 family transposase | - |
| M5T24_RS24705 (99976) | 99976..100680 | + | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| M5T24_RS24710 (100874) | 100874..101224 | - | 351 | Protein_115 | 60 kDa chaperonin | VF |
| M5T24_RS24715 (101287) | 101287..101835 | + | 549 | WP_014342204.1 | cation:proton antiporter | - |
| M5T24_RS24720 (101916) | 101916..102671 | - | 756 | WP_012372818.1 | 16S rRNA (guanine(1405)-N(7))-methyltransferase | ARG |
| M5T24_RS24725 (102841) | 102841..103701 | - | 861 | WP_000027057.1 | Beta-lactamase TEM | ARG |
| M5T24_RS24730 (103884) | 103884..104441 | - | 558 | WP_001235713.1 | Tn3 family transposase TnpR_Tn3 | Transposase |
| M5T24_RS24735 (104743) | 104743..105507 | + | 765 | WP_001389365.1 | IS6-like element IS6100 family transposase | - |
| M5T24_RS24740 (106000) | 106000..106584 | - | 585 | WP_001137892.1 | macrolide-binding transcriptional repressor MphR(A) | - |
| M5T24_RS24745 (106584) | 106584..107822 | - | 1239 | WP_000004159.1 | macrolide resistance MFS transporter Mrx(A) | - |
| M5T24_RS24750 (107819) | 107819..108733 | - | 915 | WP_023063803.1 | hypothetical protein | ARG |
| M5T24_RS24755 (108855) | 108855..109559 | - | 705 | WP_001067855.1 | Tn3 family transposase TnpA_ISSba14 | Transposase |
| M5T24_RS24760 (109620) | 109620..112031 | + | 2412 | Protein_125 | Tn3 family transposase TnpA_ISSba14 | Transposase |
| M5T24_RS24765 (112065) | 112065..112169 | + | 105 | Protein_126 | IS5/IS1182 family transposase | - |
| M5T24_RS24770 (112319) | 112319..112951 | - | 633 | Protein_127 | histidine-type phosphatase | - |
| M5T24_RS24775 (113073) | 113073..113558 | - | 486 | WP_000874189.1 | hypothetical protein | - |
| M5T24_RS24780 (113583) | 113583..114068 | - | 486 | WP_001267176.1 | TlpA disulfide reductase family protein | - |
| M5T24_RS24785 (114055) | 114055..114750 | - | 696 | WP_000117262.1 | Lipoprotein-releasing system ATP-binding protein LolD | VF |
| M5T24_RS24790 (114755) | 114755..115885 | - | 1131 | WP_000729219.1 | ABC transporter permease | - |
| M5T24_RS24795 (115875) | 115875..117158 | - | 1284 | WP_000964653.1 | ABC transporter permease | - |
| M5T24_RS24800 (117161) | 117161..118540 | - | 1380 | WP_000119836.1 | Fe-S-containing protein | - |
| M5T24_RS24805 (118644) | 118644..119171 | - | 528 | WP_000178050.1 | iron transporter | - |
| M5T24_RS24810 (119212) | 119212..120840 | - | 1629 | Protein_135 | FTR1 family iron permease | - |
| M5T24_RS24815 (121035) | 121035..122576 | + | 1542 | WP_002431311.1 | IS21 family transposase ISEc12 | Transposase |
| M5T24_RS24820 (122591) | 122591..123337 | + | 747 | WP_001016257.1 | IS21 family transposase ISEc12 | Transposase |
| M5T24_RS24825 (123415) | 123415..123684 | - | 270 | Protein_138 | iron permease | - |
| M5T24_RS24830 (124031) | 124031..124792 | - | 762 | WP_259426027.1 | Na(+)-translocating NADH-quinone reductase subunit C | - |
| M5T24_RS24835 (125029) | 125029..125535 | - | 507 | WP_000949452.1 | protein disulfide oxidoreductase | - |
| M5T24_RS24840 (125525) | 125525..125695 | - | 171 | Protein_141 | DsbA family protein | - |
| M5T24_RS24845 (125833) | 125833..125946 | - | 114 | Protein_142 | IS6 family transposase | - |
| M5T24_RS24850 (126146) | 126146..126843 | + | 698 | WP_223350503.1 | IS1 family transposase IS1X2 | Transposase |
| M5T24_RS24855 (126993) | 126993..127679 | + | 687 | Protein_144 | IS1 family transposase IS1S | Transposase |
| M5T24_RS24860 (127933) | 127933..128955 | - | 1023 | WP_000361402.1 | helicase UvrD | - |
| M5T24_RS24865 (128940) | 128940..130505 | - | 1566 | WP_001128474.1 | AAA family ATPase | - |
| M5T24_RS24870 (130580) | 130580..130996 | - | 417 | WP_001034044.1 | type II toxin-antitoxin system VapC family toxin | - |
| M5T24_RS24875 (130993) | 130993..131223 | - | 231 | WP_001261286.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| M5T24_RS24880 (131783) | 131783..132001 | + | 219 | WP_000813630.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| M5T24_RS24885 (132003) | 132003..132308 | + | 306 | WP_001159871.1 | type II toxin-antitoxin system toxin CcdB | - |
| M5T24_RS24890 (132309) | 132309..133115 | + | 807 | WP_000016970.1 | site-specific integrase | - |
Similar MGE(s)
Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE.
| Detail | Organism | MGE type | Related TA | Genome accession | Coordinates | Mash distance |
|---|