MGE detailed information
MGE type: Conjugative plasmid MGE length: 137403 bp
Accession: NZ_CP055200 Location: 1..137403 Organism: Shigella flexneri strain STEFF_3 Replicon: plasmid unnamed1
Accession: NZ_CP055200 Location: 1..137403 Organism: Shigella flexneri strain STEFF_3 Replicon: plasmid unnamed1
Gene structure
The 5 kb flanking regions of this MGE are displayed.
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HUZ39_RS24005 (1) | 1..978 | + | 978 | WP_000361611.1 | RepB family plasmid replication initiator protein | - |
| HUZ39_RS24010 (1263) | 1263..2003 | - | 741 | WP_001066953.1 | tyrosine-type recombinase/integrase | - |
| HUZ39_RS24015 (2124) | 2124..2319 | - | 196 | Protein_2 | serine/threonine protein kinase | - |
| HUZ39_RS24020 (2406) | 2406..2816 | - | 411 | Protein_3 | IS3 family transposase ISEhe3 | Transposase |
| HUZ39_RS24025 (2879) | 2879..3583 | + | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| HUZ39_RS24030 (3637) | 3637..4104 | + | 468 | Protein_5 | conjugal transfer protein | - |
| HUZ39_RS24035 (4349) | 4349..4465 | + | 117 | WP_001814923.1 | tetracycline resistance determinant leader peptide | - |
| HUZ39_RS24040 (4481) | 4481..6400 | + | 1920 | WP_025989258.1 | Tetracycline resistance protein TetM from transposon TnFO1 | VF |
| HUZ39_RS24045 (6519) | 6519..6686 | + | 168 | WP_000336323.1 | cysteine-rich KTR domain-containing protein | - |
| HUZ39_RS24050 (6947) | 6947..7579 | + | 633 | WP_020802982.1 | IS256 family transposase IS406 | Transposase |
| HUZ39_RS24055 (7826) | 7826..8158 | - | 333 | WP_000800531.1 | quaternary ammonium compound efflux SMR transporter QacL | - |
| HUZ39_RS24060 (8281) | 8281..9119 | - | 839 | Protein_11 | Streptomycin 3''-adenylyltransferase | ARG |
| HUZ39_RS24065 (9212) | 9212..10471 | - | 1260 | WP_000095725.1 | Bicyclomycin resistance protein | ARG |
| HUZ39_RS24070 (10686) | 10686..11524 | - | 839 | Protein_13 | Streptomycin 3''-adenylyltransferase | ARG |
| HUZ39_RS24075 (11530) | 11530..11820 | - | 291 | WP_001336345.1 | DUF1010 domain-containing protein | - |
| HUZ39_RS24080 (11932) | 11932..12429 | - | 498 | WP_001083725.1 | Dihydrofolate reductase | ARG |
| HUZ39_RS24085 (12574) | 12574..13587 | + | 1014 | WP_000845039.1 | IS91 family transposase ISAzo26 | Transposase |
| HUZ39_RS24090 (13790) | 13790..14140 | + | 351 | WP_071830595.1 | DUF3330 domain-containing protein | - |
| HUZ39_RS24095 (14266) | 14266..14826 | + | 561 | WP_000147567.1 | Tn3 family transposase TnpR_TnAs3 | Transposase |
| HUZ39_RS24100 (14829) | 14829..17780 | + | 2952 | WP_015344976.1 | Tn3 family transposase TnpA_TnAs3 | Transposase |
| HUZ39_RS24105 (17714) | 17714..18190 | + | 477 | WP_223171565.1 | hypothetical protein | - |
| HUZ39_RS24110 (18275) | 18275..18979 | - | 705 | WP_001067784.1 | IS6 family transposase IS1006 | Transposase |
| HUZ39_RS24115 (19043) | 19043..19873 | + | 831 | Protein_22 | IS91 family transposase ISVsa3 | Transposase |
| HUZ39_RS24120 (19904) | 19904..20788 | + | 885 | WP_012728215.1 | DUF3363 domain-containing protein | - |
| HUZ39_RS24125 (21005) | 21005..22219 | + | 1215 | WP_058657119.1 | Bicyclomycin resistance protein | ARG |
| HUZ39_RS24130 (22247) | 22247..22552 | + | 306 | WP_001255015.1 | HTH-type transcriptional regulator CynR | VF |
| HUZ39_RS24135 (22664) | 22664..22852 | + | 189 | Protein_26 | IS91 family transposase | - |
| HUZ39_RS24140 (22819) | 22819..23289 | - | 471 | Protein_27 | MFS transporter | - |
| HUZ39_RS24145 (23353) | 23353..24057 | + | 705 | WP_001067858.1 | IS6 family transposase IS15DI | Transposase |
| HUZ39_RS24150 (24109) | 24109..24426 | + | 318 | Protein_29 | recombinase family protein | - |
| HUZ39_RS24155 (24530) | 24530..25504 | + | 975 | WP_012478345.1 | IS30 family transposase ISApl1 | Transposase |
| HUZ39_RS24160 (25681) | 25681..26541 | + | 861 | WP_021561477.1 | Beta-lactamase TEM | ARG |
| HUZ39_RS24165 (26799) | 26799..27590 | - | 792 | WP_000034420.1 | Dihydropteroate synthase | ARG |
| HUZ39_RS24170 (28059) | 28059..28304 | - | 246 | WP_001354008.1 | GrpB family protein | - |
| HUZ39_RS24175 (28342) | 28342..29205 | - | 864 | WP_000612791.1 | SDR family oxidoreductase | - |
| HUZ39_RS24180 (29351) | 29351..29602 | - | 252 | Protein_35 | hypothetical protein | - |
| HUZ39_RS24185 (29654) | 29654..30358 | - | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| HUZ39_RS24190 (30509) | 30509..31324 | + | 816 | WP_000018329.1 | Aminoglycoside 3'-phosphotransferase | ARG |
| HUZ39_RS24195 (31514) | 31514..32218 | - | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| HUZ39_RS24200 (32327) | 32327..32584 | - | 258 | WP_231363335.1 | hypothetical protein | - |
| HUZ39_RS24205 (32686) | 32686..34347 | - | 1662 | Protein_40 | Tn3 family transposase TnpA_TnAs1 | Transposase |
| HUZ39_RS24210 (34310) | 34310..34744 | + | 435 | WP_223963367.1 | isochorismatase family cysteine hydrolase | - |
| HUZ39_RS24215 (34882) | 34882..35766 | + | 885 | WP_000058717.1 | EamA family transporter | - |
| HUZ39_RS24220 (35798) | 35798..36997 | - | 1200 | WP_001493765.1 | Tetracycline resistance protein, class C | ARG |
| HUZ39_RS24225 (37103) | 37103..37753 | + | 651 | WP_001493764.1 | tetracycline resistance transcriptional repressor TetR(A) | - |
| HUZ39_RS24230 (37785) | 37785..38027 | - | 243 | WP_000844627.1 | transposase | - |
| HUZ39_RS24235 (38085) | 38085..39599 | - | 1515 | Protein_46 | Tn3 family transposase TnpA_TnAs1 | Transposase |
| HUZ39_RS24240 (39665) | 39665..40369 | + | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| HUZ39_RS24245 (40617) | 40617..40904 | + | 288 | WP_230071785.1 | hypothetical protein | - |
| HUZ39_RS24255 (42281) | 42281..43096 | + | 816 | WP_001043260.1 | Dihydropteroate synthase | ARG |
| HUZ39_RS24260 (43183) | 43183..43425 | + | 243 | Protein_51 | phosphoglucosamine mutase | - |
| HUZ39_RS24265 (43661) | 43661..45154 | - | 1494 | WP_015344975.1 | IS91 family transposase ISVsa3 | Transposase |
| HUZ39_RS24270 (45365) | 45365..45589 | - | 225 | WP_000743213.1 | hypothetical protein | - |
| HUZ39_RS24275 (45586) | 45586..46296 | - | 711 | WP_194433555.1 | Tn3 family transposase TnpR_TnEc1 | Transposase |
| HUZ39_RS24280 (46716) | 46716..47621 | + | 906 | WP_052259183.1 | rRNA adenine N-6-methyltransferase | ARG |
| HUZ39_RS24285 (47772) | 47772..47951 | + | 180 | Protein_56 | helix-turn-helix domain-containing protein | - |
| HUZ39_RS24290 (48016) | 48016..48720 | + | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| HUZ39_RS24295 (49015) | 49015..50229 | + | 1215 | WP_001532073.1 | type II site-specific deoxyribonuclease | - |
| HUZ39_RS24300 (50263) | 50263..51696 | - | 1434 | WP_001288435.1 | DNA cytosine methyltransferase | - |
| HUZ39_RS24305 (51790) | 51790..52062 | + | 273 | Protein_60 | IS1 family transposase | - |
| HUZ39_RS24310 (52319) | 52319..53016 | + | 698 | WP_095033700.1 | IS1 family transposase IS1R | Transposase |
| HUZ39_RS24315 (53137) | 53137..53975 | - | 839 | Protein_62 | Streptomycin 3''-adenylyltransferase | ARG |
| HUZ39_RS24320 (54121) | 54121..54801 | + | 681 | Protein_63 | IS91 family transposase ISAzo26 | Transposase |
| HUZ39_RS24325 (54866) | 54866..55570 | + | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| HUZ39_RS24330 (55636) | 55636..56181 | + | 546 | Protein_65 | Tn3 family transposase TnpA_Tn3 | Transposase |
| HUZ39_RS24335 (56217) | 56217..56632 | - | 416 | Protein_66 | IS1-like element IS1B family transposase | - |
| HUZ39_RS24340 (56881) | 56881..57282 | - | 402 | WP_001398199.1 | type II toxin-antitoxin system toxin endoribonuclease PemK | - |
| HUZ39_RS24345 (57215) | 57215..57472 | - | 258 | WP_000557619.1 | type II toxin-antitoxin system antitoxin PemI | - |
| HUZ39_RS24350 (57565) | 57565..58218 | - | 654 | WP_000616807.1 | CPBP family intramembrane metalloprotease | - |
| HUZ39_RS24355 (59032) | 59032..60603 | - | 1572 | WP_000381395.1 | IS66 family transposase ISCro1 | Transposase |
| HUZ39_RS24360 (60623) | 60623..60970 | - | 348 | WP_000624622.1 | IS66 family transposase ISCro1 | Transposase |
| HUZ39_RS24365 (60970) | 60970..61647 | - | 678 | WP_001682408.1 | IS66 family transposase ISCro1 | Transposase |
| HUZ39_RS24370 (61864) | 61864..62721 | - | 858 | WP_000130646.1 | incFII family plasmid replication initiator RepA | - |
| HUZ39_RS24375 (62714) | 62714..62788 | - | 75 | WP_001365705.1 | RepA leader peptide Tap | - |
| HUZ39_RS24380 (63026) | 63026..63280 | - | 255 | WP_000083830.1 | replication regulatory protein RepA | - |
| HUZ39_RS24385 (63520) | 63520..64110 | - | 591 | WP_000766807.1 | DUF2726 domain-containing protein | - |
| HUZ39_RS24390 (64148) | 64148..64357 | - | 210 | WP_001298565.1 | hemolysin expression modulator Hha | - |
| HUZ39_RS24395 (64403) | 64403..64864 | - | 462 | WP_001233838.1 | thermonuclease family protein | - |
| HUZ39_RS24400 (65109) | 65109..65321 | - | 213 | WP_001309245.1 | ANR family transcriptional regulator | - |
| HUZ39_RS24405 (65450) | 65450..66010 | - | 561 | WP_000139323.1 | fertility inhibition protein FinO | - |
| HUZ39_RS24410 (66113) | 66113..66973 | - | 861 | WP_000704523.1 | putative protein YcjY | VF |
| HUZ39_RS24415 (67032) | 67032..67778 | - | 747 | WP_000205725.1 | conjugal transfer pilus acetylase TraX | - |
| HUZ39_RS24420 (67798) | 67798..73068 | - | 5271 | WP_239637979.1 | Multifunctional conjugation protein TraI | Relaxase |
| HUZ39_RS24425 (73068) | 73068..75257 | - | 2190 | WP_017901267.1 | type IV conjugative transfer system coupling protein TraD | - |
| HUZ39_RS24430 (75308) | 75308..76045 | - | 738 | WP_000199905.1 | hypothetical protein | - |
| HUZ39_RS24435 (76248) | 76248..76979 | - | 732 | WP_000782451.1 | conjugal transfer complement resistance protein TraT | - |
| HUZ39_RS24440 (77028) | 77028..77513 | - | 486 | WP_000605870.1 | hypothetical protein | - |
| HUZ39_RS24445 (77529) | 77529..80351 | - | 2823 | WP_001007039.1 | conjugal transfer mating-pair stabilization protein TraG | - |
| HUZ39_RS24450 (80348) | 80348..81721 | - | 1374 | WP_000944331.1 | conjugal transfer pilus assembly protein TraH | - |
| HUZ39_RS24455 (81708) | 81708..82100 | - | 393 | WP_000660699.1 | F-type conjugal transfer protein TrbF | - |
| HUZ39_RS24460 (82081) | 82081..82428 | - | 348 | WP_001309242.1 | P-type conjugative transfer protein TrbJ | - |
| HUZ39_RS24465 (82358) | 82358..82903 | - | 546 | WP_000059831.1 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | - |
| HUZ39_RS24470 (82890) | 82890..83174 | - | 285 | WP_000624194.1 | type-F conjugative transfer system pilin chaperone TraQ | - |
| HUZ39_RS24475 (83293) | 83293..83637 | - | 345 | WP_000556796.1 | conjugal transfer protein TrbA | - |
| HUZ39_RS24480 (83651) | 83651..84394 | - | 744 | WP_001030371.1 | type-F conjugative transfer system pilin assembly protein TraF | - |
| HUZ39_RS24485 (84387) | 84387..84644 | - | 258 | WP_000864353.1 | conjugal transfer protein TrbE | - |
| HUZ39_RS24490 (84671) | 84671..86521 | - | 1851 | WP_000821856.1 | type-F conjugative transfer system mating-pair stabilization protein TraN | - |
| HUZ39_RS24495 (86518) | 86518..87156 | - | 639 | WP_001080256.1 | type-F conjugative transfer system pilin assembly protein TrbC | - |
| HUZ39_RS24500 (87165) | 87165..87470 | - | 306 | WP_000224416.1 | hypothetical protein | - |
| HUZ39_RS24505 (87500) | 87500..88492 | - | 993 | WP_000830838.1 | conjugal transfer pilus assembly protein TraU | - |
| HUZ39_RS24510 (88489) | 88489..89121 | - | 633 | WP_001203728.1 | type-F conjugative transfer system protein TraW | - |
| HUZ39_RS24515 (89118) | 89118..89504 | - | 387 | WP_000214084.1 | type-F conjugative transfer system protein TrbI | - |
| HUZ39_RS24520 (89501) | 89501..91312 | - | 1812 | Protein_103 | type IV secretion system protein TraC | - |
| HUZ39_RS24525 (91502) | 91502..93043 | + | 1542 | WP_001298859.1 | IS21 family transposase ISEc12 | Transposase |
| HUZ39_RS24530 (93058) | 93058..93804 | + | 747 | WP_001016257.1 | IS21 family transposase ISEc12 | Transposase |
| HUZ39_RS24535 (93815) | 93815..94717 | - | 903 | Protein_106 | TraC family protein | - |
| HUZ39_RS24540 (94843) | 94843..95190 | - | 348 | WP_000836682.1 | hypothetical protein | - |
| HUZ39_RS24545 (95218) | 95218..95436 | - | 219 | WP_000556745.1 | hypothetical protein | - |
| HUZ39_RS24550 (95516) | 95516..95998 | - | 483 | WP_204084453.1 | hypothetical protein | - |
| HUZ39_RS24555 (95991) | 95991..96404 | - | 414 | WP_000549589.1 | hypothetical protein | - |
| HUZ39_RS24560 (96397) | 96397..96618 | - | 222 | WP_001278683.1 | conjugal transfer protein TraR | - |
| HUZ39_RS24565 (96753) | 96753..97268 | - | 516 | WP_000809881.1 | type IV conjugative transfer system lipoprotein TraV | - |
| HUZ39_RS24570 (97265) | 97265..97585 | - | 321 | WP_001057307.1 | conjugal transfer protein TrbD | - |
| HUZ39_RS24575 (97572) | 97572..98138 | - | 567 | WP_000896599.1 | conjugal transfer pilus-stabilizing protein TraP | - |
| HUZ39_RS24580 (98128) | 98128..99579 | - | 1452 | WP_000146675.1 | F-type conjugal transfer pilus assembly protein TraB | - |
| HUZ39_RS24585 (99579) | 99579..100307 | - | 729 | WP_001230772.1 | type-F conjugative transfer system secretin TraK | - |
| HUZ39_RS24590 (100294) | 100294..100860 | - | 567 | WP_000399780.1 | type IV conjugative transfer system protein TraE | - |
| HUZ39_RS24595 (100882) | 100882..101193 | - | 312 | WP_000012113.1 | type IV conjugative transfer system protein TraL | - |
| HUZ39_RS24600 (101208) | 101208..101567 | - | 360 | WP_001098992.1 | type IV conjugative transfer system pilin TraA | - |
| HUZ39_RS24605 (101600) | 101600..101827 | - | 228 | WP_000089263.1 | conjugal transfer relaxosome protein TraY | - |
| HUZ39_RS24610 (101964) | 101964..102635 | - | 672 | WP_000283561.1 | conjugal transfer transcriptional regulator TraJ | - |
| HUZ39_RS24615 (102829) | 102829..103212 | - | 384 | WP_001354030.1 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| HUZ39_RS24620 (103547) | 103547..104137 | + | 591 | WP_000252683.1 | hypothetical protein | VF |
| HUZ39_RS24625 (104434) | 104434..105255 | - | 822 | WP_001234445.1 | DUF932 domain-containing protein | - |
| HUZ39_RS24630 (105366) | 105366..105662 | - | 297 | WP_001272251.1 | hypothetical protein | - |
| HUZ39_RS24635 (105962) | 105962..106258 | + | 297 | Protein_126 | hypothetical protein | - |
| HUZ39_RS24640 (106577) | 106577..106702 | - | 126 | WP_001372321.1 | type I toxin-antitoxin system Hok family toxin | Toxin Detail |
| HUZ39_RS24645 (106644) | 106644..106793 | - | 150 | Protein_128 | plasmid maintenance protein Mok | - |
| HUZ39_RS24650 (106815) | 106815..106994 | + | 180 | WP_001309233.1 | hypothetical protein | - |
| - (106779) | 106779..107003 | - | 225 | NuclAT_0 | - | Antitoxin Detail |
| - (106779) | 106779..107003 | - | 225 | NuclAT_0 | - | Antitoxin Detail |
| - (106779) | 106779..107003 | - | 225 | NuclAT_0 | - | Antitoxin Detail |
| - (106779) | 106779..107003 | - | 225 | NuclAT_0 | - | Antitoxin Detail |
| HUZ39_RS24655 (107015) | 107015..107734 | - | 720 | WP_001276217.1 | plasmid SOS inhibition protein A | - |
| HUZ39_RS24660 (107731) | 107731..108165 | - | 435 | WP_000845953.1 | conjugation system SOS inhibitor PsiB | - |
| HUZ39_RS24665 (108234) | 108234..110257 | - | 2024 | Protein_132 | ParB/RepB/Spo0J family partition protein | - |
| HUZ39_RS24670 (110318) | 110318..110551 | - | 234 | WP_000006003.1 | DUF905 family protein | - |
| HUZ39_RS24675 (110609) | 110609..111136 | - | 528 | WP_000290834.1 | single-stranded DNA-binding protein | - |
| HUZ39_RS24680 (111438) | 111438..111887 | + | 450 | Protein_135 | hypothetical protein | - |
| HUZ39_RS24685 (111973) | 111973..112536 | - | 564 | WP_001298559.1 | class I SAM-dependent methyltransferase | - |
| HUZ39_RS24690 (112583) | 112583..113944 | - | 1362 | WP_000170695.1 | DUF3560 domain-containing protein | - |
| HUZ39_RS24695 (113996) | 113996..114226 | - | 231 | WP_000218642.1 | hypothetical protein | - |
| HUZ39_RS24700 (114743) | 114743..114991 | + | 249 | WP_077249352.1 | hypothetical protein | - |
| HUZ39_RS24705 (115264) | 115264..115455 | - | 192 | WP_001027516.1 | hypothetical protein | - |
| HUZ39_RS24710 (115452) | 115452..115874 | - | 423 | WP_000271753.1 | DUF1380 family protein | - |
| HUZ39_RS24715 (115921) | 115921..116346 | - | 426 | WP_001198928.1 | antirestriction protein | - |
| HUZ39_RS24720 (116763) | 116763..117539 | - | 777 | WP_001753849.1 | hypothetical protein | - |
| HUZ39_RS24725 (117590) | 117590..118108 | - | 519 | WP_123325260.1 | DUF1380 family protein | - |
| HUZ39_RS24730 (118038) | 118038..118259 | - | 222 | WP_001104873.1 | hypothetical protein | - |
| HUZ39_RS24735 (118260) | 118260..118943 | - | 684 | WP_000086185.1 | DNA methylase | - |
| HUZ39_RS24740 (119020) | 119020..119331 | - | 312 | WP_011254640.1 | hypothetical protein | - |
| HUZ39_RS24745 (119328) | 119328..120230 | - | 903 | WP_001348615.1 | DUF1281 domain-containing protein | - |
| HUZ39_RS24750 (121097) | 121097..122068 | - | 972 | WP_000817036.1 | ParB/RepB/Spo0J family plasmid partition protein | - |
| HUZ39_RS24755 (122068) | 122068..123234 | - | 1167 | WP_000772446.1 | Sporulation initiation inhibitor protein Soj | VF |
| HUZ39_RS24760 (123822) | 123822..124577 | - | 756 | WP_000852146.1 | replication initiation protein RepE | - |
| HUZ39_RS24765 (125351) | 125351..126157 | - | 807 | WP_000016982.1 | site-specific integrase | - |
| HUZ39_RS24770 (126158) | 126158..126463 | - | 306 | WP_001159868.1 | type II toxin-antitoxin system toxin CcdB | Toxin Detail |
| HUZ39_RS24775 (126465) | 126465..126683 | - | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin Detail |
| HUZ39_RS24780 (127275) | 127275..127763 | + | 489 | WP_011254646.1 | hypothetical protein | - |
| HUZ39_RS24785 (127797) | 127797..128930 | - | 1134 | WP_001542067.1 | DUF3800 domain-containing protein | - |
| HUZ39_RS24790 (129097) | 129097..129870 | - | 774 | WP_000905949.1 | hypothetical protein | - |
| HUZ39_RS24795 (129883) | 129883..130383 | - | 501 | WP_000528931.1 | HEPN family nuclease | - |
| HUZ39_RS24800 (130648) | 130648..130878 | + | 231 | WP_001261278.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin Detail |
| HUZ39_RS24805 (130875) | 130875..131291 | + | 417 | WP_001034046.1 | type II toxin-antitoxin system VapC family toxin | Toxin Detail |
| HUZ39_RS24810 (131336) | 131336..134671 | - | 3336 | WP_239637978.1 | pcar | - |
| HUZ39_RS24815 (134778) | 134778..135475 | + | 698 | WP_223155668.1 | IS1 family transposase IS1S | Transposase |
| HUZ39_RS24820 (135655) | 135655..136168 | - | 514 | Protein_163 | DUF4113 domain-containing protein | - |
| HUZ39_RS24825 (136229) | 136229..136333 | - | 105 | Protein_164 | AAA family ATPase | - |
| HUZ39_RS24830 (136335) | 136335..136658 | - | 324 | Protein_165 | IS21 family transposase IS21 | Transposase |
Similar MGE(s)
Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE.
| Detail | Organism | MGE type | Related TA | Genome accession | Coordinates | Mash distance |
|---|