Detailed information of TA system TA161919

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 106577..107003
• Replicon: plasmid unnamed1
• Accession: NZ_CP055200
• Organism: Shigella flexneri strain STEFF_3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HUZ39_RS24640
• Protein ID: WP_001372321.1
• Coordinates: 106577..106702 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	106779..107003 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ39_RS24605 (101600)	101600..101827	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
HUZ39_RS24610 (101964)	101964..102635	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
HUZ39_RS24615 (102829)	102829..103212	-	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HUZ39_RS24620 (103547)	103547..104137	+	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
HUZ39_RS24625 (104434)	104434..105255	-	822	WP_001234445.1	DUF932 domain-containing protein	-
HUZ39_RS24630 (105366)	105366..105662	-	297	WP_001272251.1	hypothetical protein	-
HUZ39_RS24635 (105962)	105962..106258	+	297	Protein_126	hypothetical protein	-
HUZ39_RS24640 (106577)	106577..106702	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUZ39_RS24645 (106644)	106644..106793	-	150	Protein_128	plasmid maintenance protein Mok	-
HUZ39_RS24650 (106815)	106815..106994	+	180	WP_001309233.1	hypothetical protein	-
- (106779)	106779..107003	-	225	NuclAT_0	-	Antitoxin
- (106779)	106779..107003	-	225	NuclAT_0	-	Antitoxin
- (106779)	106779..107003	-	225	NuclAT_0	-	Antitoxin
- (106779)	106779..107003	-	225	NuclAT_0	-	Antitoxin
HUZ39_RS24655 (107015)	107015..107734	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
HUZ39_RS24660 (107731)	107731..108165	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HUZ39_RS24665 (108234)	108234..110257	-	2024	Protein_132	ParB/RepB/Spo0J family partition protein	-
HUZ39_RS24670 (110318)	110318..110551	-	234	WP_000006003.1	DUF905 family protein	-
HUZ39_RS24675 (110609)	110609..111136	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
HUZ39_RS24680 (111438)	111438..111887	+	450	Protein_135	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / blaTEM-30 / sul3 / aph(3')-Ia / tet(A) / sul2 / erm(42)	-	1..137403	137403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T161919 WP_001372321.1 NZ_CP055200:c106702-106577 [Shigella flexneri]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T161919 NZ_CP071409:2905287-2905505 [[Pantoea] beijingensis]
ATGACTGACAAGACTCTTACTAAAACTGATTATCTAATGCGCTTAAGACGTTGTCGCTCGATTGACACGCTTGAACGAGT
GATTGAAAAAAATAAATATGAATTATCTGATGATGAATTAGAAGTATTCAATTCCGCAGCGGACCACCGTCTGGCTGAGC
TAACAATGAATAAACTCTATGACAAAGTCCCCGTTTCCGTATGGAAATATGTTCGCTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT161919 NZ_CP055200:c107003-106779 [Shigella flexneri]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin