Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 130648..131291 Replicon plasmid unnamed1
Accession NZ_CP055200
Organism Shigella flexneri strain STEFF_3

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag HUZ39_RS24805 Protein ID WP_001034046.1
Coordinates 130875..131291 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag HUZ39_RS24800 Protein ID WP_001261278.1
Coordinates 130648..130878 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ39_RS24770 (126158) 126158..126463 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
HUZ39_RS24775 (126465) 126465..126683 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
HUZ39_RS24780 (127275) 127275..127763 + 489 WP_011254646.1 hypothetical protein -
HUZ39_RS24785 (127797) 127797..128930 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
HUZ39_RS24790 (129097) 129097..129870 - 774 WP_000905949.1 hypothetical protein -
HUZ39_RS24795 (129883) 129883..130383 - 501 WP_000528931.1 HEPN family nuclease -
HUZ39_RS24800 (130648) 130648..130878 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUZ39_RS24805 (130875) 130875..131291 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ39_RS24810 (131336) 131336..134671 - 3336 WP_239637978.1 pcar -
HUZ39_RS24815 (134778) 134778..135475 + 698 WP_223155668.1 IS1-like element IS1A family transposase -
HUZ39_RS24820 (135655) 135655..136168 - 514 Protein_163 DUF4113 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / blaTEM-30 / sul3 / aph(3')-Ia / tet(A) / sul2 / erm(42) - 1..137403 137403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T161923 WP_001034046.1 NZ_CP055200:130875-131291 [Shigella flexneri]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T161923 NZ_CP071436:711982-712278 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT161923 WP_001261278.1 NZ_CP055200:130648-130878 [Shigella flexneri]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT161923 NZ_CP071436:712280-712675 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References