MGE detailed information

MGE type: Conjugative plasmid        MGE length: 170395 bp
Accession: NZ_CP048311        Location: 1..170395         Organism: Escherichia coli strain 32-4       Replicon: plasmid p32-4_A


Gene structure


The 5 kb flanking regions of this MGE are displayed.

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS16_RS23190 () 1..858 + 858 WP_013362810.1 incFII family plasmid replication initiator RepA -
GZS16_RS23195 () 1559..1699 + 141 WP_001333237.1 hypothetical protein -
GZS16_RS23200 () 1797..2450 + 654 WP_000616807.1 CPBP family intramembrane metalloprotease -
GZS16_RS23205 () 2543..2800 + 258 WP_000557619.1 type II toxin-antitoxin system antitoxin PemI -
GZS16_RS23210 () 2733..3134 + 402 WP_001398199.1 type II toxin-antitoxin system toxin endoribonuclease PemK -
GZS16_RS23215 () 3383..3797 + 415 Protein_6 IS1 family transposase -
GZS16_RS23220 () 3833..4377 - 545 Protein_7 Tn3 family transposase TnpA_Tn3 Transposase
GZS16_RS23225 () 4443..5146 - 704 Protein_8 IS6 family transposase IS15DII Transposase
GZS16_RS23230 () 5158..6639 - 1482 WP_024210412.1 IS91 family transposase ISVsa3 Transposase
GZS16_RS25280 () 6866..7207 - 342 WP_023909256.1 60 kDa chaperonin 2 VF
GZS16_RS23240 () 7168..7617 - 450 WP_013362817.1 hypothetical protein -
GZS16_RS25285 () 8132..8302 - 171 WP_170852050.1 rRNA adenine methyltransferase -
GZS16_RS23245 () 8247..8984 - 738 WP_013362818.1 rRNA adenine N-6-methyltransferase ARG
GZS16_RS23250 () 9110..9205 - 96 WP_013362819.1 23S rRNA methyltransferase attenuation leader peptide -
GZS16_RS23255 () 9340..10033 + 694 Protein_15 IS6 family transposase IS15DI Transposase
GZS16_RS23260 () 10162..11076 + 915 WP_110498949.1 hypothetical protein ARG
GZS16_RS23265 () 11073..12309 + 1237 Protein_17 MFS transporter -
GZS16_RS23270 () 12309..12888 + 580 Protein_18 TetR/AcrR family transcriptional regulator -
GZS16_RS23275 () 13328..14023 - 696 WP_158192044.1 IS6 family transposase IS15DII Transposase
GZS16_RS23280 () 14212..14908 + 697 Protein_20 IS1 family transposase IS1R Transposase
GZS16_RS23285 () 14905..15168 + 264 WP_049802299.1 antibiotic biosynthesis monooxygenase -
GZS16_RS23290 () 15161..15547 + 387 WP_000460651.1 hypothetical protein -
GZS16_RS23295 () 15555..16241 + 687 WP_001284954.1 winged helix-turn-helix domain-containing protein -
GZS16_RS23300 () 16219..16842 - 624 WP_000088605.1 Tetracycline repressor protein class B from transposon Tn10 VF
GZS16_RS23305 () 16924..18129 + 1206 WP_001089068.1 Tetracycline resistance protein, class B ARG
GZS16_RS23310 () 18242..18835 - 594 WP_000428546.1 tetracyline resistance-associated transcriptional repressor TetC -
GZS16_RS23315 () 18968..19339 + 372 WP_035689358.1 Transposon Tn10 TetD protein VF
GZS16_RS23320 () 19349..20233 - 885 Protein_28 IS4-like element ISVsa5 family transposase -
GZS16_RS23325 () 20330..20990 - 661 Protein_29 IS6 family transposase IS15DII Transposase
GZS16_RS23335 () 21331..21534 - 204 WP_064765192.1 hypothetical protein -
GZS16_RS23340 () 21731..22570 - 840 WP_000259031.1 Dihydropteroate synthase ARG
GZS16_RS23345 () 22564..22911 - 348 WP_000679427.1 Multidrug transporter EmrE ARG
GZS16_RS23350 () 23117..23905 - 789 WP_000503573.1 Streptomycin 3''-adenylyltransferase ARG
GZS16_RS23355 () 24036..24509 - 474 WP_001389366.1 trimethoprim-resistant dihydrofolate reductase DfrA17 -
GZS16_RS23360 () 24667..25680 + 1014 WP_000845048.1 IS91 family transposase ISAzo26 Transposase
GZS16_RS25290 () 25883..26173 + 291 WP_032152941.1 DUF3330 domain-containing protein -
GZS16_RS23370 () 26210..26914 + 705 Protein_37 IS6 family transposase -
GZS16_RS23375 () 26968..27936 + 969 Protein_38 IS1380 family transposase ISEcp1 Transposase
GZS16_RS23380 () 28190..29065 + 876 WP_000239590.1 Beta-lactamase CTX-M-1 ARG
GZS16_RS23385 () 29112..29444 - 333 WP_001393253.1 WbuC family cupin fold metalloprotein -
GZS16_RS23390 () 29458..31701 + 2244 Protein_41 Tn3 family transposase TnpA_Tn3 Transposase
GZS16_RS23395 () 31766..32469 + 704 Protein_42 IS6 family transposase IS15DII Transposase
GZS16_RS23400 () 32515..33028 + 514 Protein_43 IS6 family transposase IS6100 Transposase
GZS16_RS23405 () 33518..34099 - 582 Protein_44 TetR/AcrR family transcriptional regulator -
GZS16_RS23410 () 34099..35333 - 1235 Protein_45 MFS transporter -
GZS16_RS23415 () 35330..36245 - 916 Protein_46 hypothetical protein ARG
GZS16_RS23420 () 36373..37068 - 696 WP_203237575.1 IS6 family transposase IS15DI Transposase
GZS16_RS23425 () 37131..37334 - 204 Protein_48 IS4 family transposase -
GZS16_RS23430 () 37327..38023 - 697 Protein_49 IS1 family transposase IS1X2 Transposase
GZS16_RS23435 () 38485..38643 + 159 WP_000449408.1 DsbA family protein -
GZS16_RS23440 () 38633..39139 + 507 WP_000949452.1 protein disulfide oxidoreductase -
GZS16_RS23445 () 39322..40137 + 816 WP_012372823.1 Na(+)-translocating NADH-quinone reductase subunit C -
GZS16_RS23450 () 40484..42370 + 1887 WP_000118029.1 FTR1 family iron permease -
GZS16_RS23455 () 42411..42938 + 528 WP_000178050.1 iron transporter -
GZS16_RS23460 () 43042..44409 + 1368 WP_162676403.1 DUF2318 domain-containing protein -
GZS16_RS23465 () 44412..45695 + 1284 WP_000964653.1 ABC transporter permease -
GZS16_RS23470 () 45685..46815 + 1131 WP_000729219.1 ABC transporter permease -
GZS16_RS23475 () 46820..47515 + 696 WP_000117262.1 Lipoprotein-releasing system ATP-binding protein LolD VF
GZS16_RS23480 () 47502..47987 + 486 WP_001267176.1 TlpA family protein disulfide reductase -
GZS16_RS23485 () 48012..48497 + 486 WP_000874189.1 hypothetical protein -
GZS16_RS23490 () 48619..49251 + 633 Protein_61 histidine-type phosphatase -
GZS16_RS23495 () 49401..49505 - 105 Protein_62 IS5/IS1182 family transposase -
GZS16_RS23500 () 49783..50787 - 1005 WP_021546935.1 IS110 family transposase IS4321R Transposase
GZS16_RS23505 () 50887..51200 - 314 Protein_64 Tn3 family transposase -
GZS16_RS23510 () 51227..51979 - 753 WP_000734115.1 alpha/beta hydrolase -
GZS16_RS23515 () 52221..53093 + 873 WP_000090196.1 DMT family transporter -
GZS16_RS23520 () 53224..54447 + 1224 WP_000872613.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -
GZS16_RS23525 () 54633..55406 - 774 WP_032152933.1 SDR family oxidoreductase -
GZS16_RS23530 () 55472..56173 - 702 WP_001194013.1 DsbA family oxidoreductase -
GZS16_RS23535 () 56239..57345 - 1107 WP_000039982.1 alkene reductase -
GZS16_RS23540 () 57559..57888 + 330 WP_002431133.1 thioredoxin family protein -
GZS16_RS23545 () 57918..58256 - 339 WP_000888080.1 carboxymuconolactone decarboxylase family protein -
GZS16_RS23550 () 58261..58842 - 582 WP_162676404.1 HTH-type transcriptional repressor NemR VF
GZS16_RS23555 () 58984..59541 - 558 WP_000108589.1 OsmC family protein -
GZS16_RS23560 () 59726..60205 - 480 Protein_75 Tn3 family transposase TnpR_ISSba14 Transposase
GZS16_RS23565 () 60303..60962 - 660 WP_162676405.1 IS6 family transposase IS15DII Transposase
GZS16_RS23570 () 60987..61361 + 375 WP_162676406.1 IS66 family transposase ISKpn24 Transposase
GZS16_RS23575 () 61410..62354 + 945 Protein_78 IS66 family transposase -
GZS16_RS23580 () 62440..63462 + 1023 WP_000255956.1 IS21 family transposase IS100kyp Transposase
GZS16_RS23585 () 63459..64239 + 781 Protein_80 IS21 family transposase IS100 Transposase
GZS16_RS23590 () 64307..64915 + 609 Protein_81 IS66 family transposase ISEc22 Transposase
GZS16_RS23595 () 64978..65767 - 790 Protein_82 DUF4198 domain-containing protein -
GZS16_RS23600 () 65774..67738 - 1965 Protein_83 TonB-dependent receptor -
GZS16_RS23605 () 67975..68453 + 479 Protein_84 transposase -
GZS16_RS23610 () 69031..69247 + 217 Protein_85 transcriptional regulator -
GZS16_RS23615 () 69693..69868 + 176 Protein_86 IS3 family transposase -
GZS16_RS23620 () 70094..70938 - 845 Protein_87 hypothetical protein -
GZS16_RS23625 () 71684..71809 + 126 WP_162676407.1 transcriptional regulator -
GZS16_RS23630 () 71930..72668 + 739 Protein_89 site-specific integrase -
GZS16_RS23635 () 72953..73926 - 974 Protein_90 RepB family plasmid replication initiator protein -
GZS16_RS23645 () 74672..74995 + 324 Protein_91 IS21 family transposase IS21 Transposase
GZS16_RS25295 () 74997..75101 + 105 Protein_92 AAA family ATPase -
GZS16_RS23650 () 75162..75656 + 495 Protein_93 DUF4113 domain-containing protein -
GZS16_RS23655 () 75852..76547 - 696 Protein_94 IS1 family transposase IS1R Transposase
GZS16_RS23660 () 76903..77817 + 915 WP_000949005.1 Putative metal ABC transporter substrate-binding protein Hpf VF
GZS16_RS23665 () 77817..78644 + 828 WP_000983710.1 Iron(3+)-hydroxamate import ATP-binding protein FhuC VF
GZS16_RS23670 () 78641..79498 + 858 WP_001101723.1 Manganese transport system membrane protein MntB ARG
GZS16_RS23675 () 79495..80352 + 858 WP_000968139.1 Manganese transport system membrane protein MntB ARG
GZS16_RS23680 () 80841..81254 + 414 Protein_99 phosphopyruvate hydratase -
GZS16_RS23685 () 81595..81975 + 381 WP_001318207.1 fluoride efflux transporter CrcB -
GZS16_RS23690 () 82355..83548 - 1194 WP_000095526.1 MFS transporter -
GZS16_RS23695 () 83684..85408 + 1725 WP_000602863.1 N(2)-citryl-N(6)-acetyl-N(6)-hydroxylysine synthase VF
GZS16_RS23700 () 85409..86356 + 948 WP_000011908.1 N(6)-hydroxylysine O-acetyltransferase VF
GZS16_RS23705 () 86356..88098 + 1743 WP_001015721.1 Aerobactin synthase VF
GZS16_RS23710 () 88095..89372 + 1278 WP_000750130.1 L-lysine N6-monooxygenase VF
GZS16_RS23715 () 89454..91655 + 2202 WP_000973519.1 Ferric aerobactin receptor VF
GZS16_RS23720 () 92181..92878 + 698 Protein_107 IS1 family transposase IS1G Transposase
GZS16_RS23725 () 93132..94154 - 1023 WP_000361402.1 helicase UvrD -
GZS16_RS23730 () 94139..95704 - 1566 WP_001128474.1 AAA family ATPase -
GZS16_RS23735 () 95779..96195 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin Detail
GZS16_RS23740 () 96192..96422 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin Detail
GZS16_RS23745 () 96982..97200 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin Detail
GZS16_RS23750 () 97202..97507 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin Detail
GZS16_RS23755 () 97508..98314 + 807 WP_000016970.1 site-specific integrase -
GZS16_RS23760 () 99036..99791 + 756 WP_000852146.1 replication initiation protein RepE -
GZS16_RS23765 () 100379..101545 + 1167 WP_000772446.1 Sporulation initiation inhibitor protein Soj VF
GZS16_RS23770 () 101545..102516 + 972 WP_000817031.1 ParB/RepB/Spo0J family plasmid partition protein -
GZS16_RS23775 () 103254..104156 + 903 WP_064766247.1 DUF1281 domain-containing protein -
GZS16_RS23780 () 104160..104464 + 305 Protein_119 hypothetical protein -
GZS16_RS23785 () 104541..105224 + 684 WP_032181561.1 DNA methylase -
GZS16_RS23790 () 105225..105446 + 222 WP_001104873.1 hypothetical protein -
GZS16_RS23795 () 105460..105894 + 435 WP_001546462.1 DUF1380 family protein -
GZS16_RS23800 () 105939..106709 + 771 WP_001006251.1 hypothetical protein -
GZS16_RS23815 () 107246..107548 + 303 WP_001666994.1 antirestriction protein -
GZS16_RS23820 () 107595..108017 + 423 WP_000271685.1 DUF1380 family protein -
GZS16_RS23825 () 108014..108205 + 192 WP_001027495.1 hypothetical protein -
GZS16_RS23830 () 108477..108725 - 249 WP_071781657.1 hypothetical protein -
GZS16_RS23835 () 109237..109467 + 231 WP_000218642.1 hypothetical protein -
GZS16_RS23840 () 109519..110880 + 1362 WP_000199439.1 DUF3560 domain-containing protein -
GZS16_RS23845 () 110928..111493 + 566 Protein_130 class I SAM-dependent methyltransferase -
GZS16_RS23850 () 111519..111725 + 207 WP_001774176.1 hypothetical protein -
GZS16_RS25300 () 112135..112341 + 207 WP_000275856.1 hypothetical protein -
GZS16_RS23860 () 112367..112906 + 540 WP_000290840.1 single-stranded DNA-binding protein -
GZS16_RS23865 () 112974..113207 + 234 WP_000005990.1 DUF905 family protein -
GZS16_RS23870 () 113235..113432 + 198 Protein_135 hypothetical protein -
GZS16_RS23875 () 113487..113921 + 435 WP_000845937.1 conjugation system SOS inhibitor PsiB -
GZS16_RS23880 () 113918..114637 + 720 WP_001276238.1 plasmid SOS inhibition protein A -
GZS16_RS23885 () 114649..114837 - 189 WP_001299721.1 hypothetical protein -
- () 114649..114846 + 198 NuclAT_0 - -
- () 114649..114846 + 198 NuclAT_0 - -
- () 114649..114846 + 198 NuclAT_0 - -
- () 114649..114846 + 198 NuclAT_0 - -
GZS16_RS23890 () 114894..116263 + 1370 WP_085947770.1 IS3 family transposase IS150 Transposase
- () 116288..116319 + 32 NuclAT_1 - -
- () 116288..116319 + 32 NuclAT_1 - -
- () 116288..116319 + 32 NuclAT_1 - -
- () 116288..116319 + 32 NuclAT_1 - -
GZS16_RS23895 () 116363..116521 + 159 WP_001312861.1 type I toxin-antitoxin system Hok family toxin Toxin Detail
GZS16_RS23905 () 117442..117729 + 288 WP_000107535.1 hypothetical protein -
GZS16_RS23910 () 117847..118668 + 822 WP_001234426.1 DUF945 domain-containing protein -
GZS16_RS23915 () 118965..119567 - 603 WP_013362798.1 hypothetical protein VF
GZS16_RS23920 () 119888..120271 + 384 WP_001151524.1 relaxosome protein TraM -
GZS16_RS23925 () 120458..121147 + 690 WP_000283385.1 conjugal transfer transcriptional regulator TraJ -
GZS16_RS23930 () 121246..121641 + 396 WP_001309237.1 conjugal transfer relaxosome protein TraY -
GZS16_RS23935 () 121674..122039 + 366 WP_000994779.1 type IV conjugative transfer system pilin TraA -
GZS16_RS23940 () 122054..122365 + 312 WP_000012106.1 type IV conjugative transfer system protein TraL -
GZS16_RS23945 () 122387..122953 + 567 WP_021514868.1 type IV conjugative transfer system protein TraE -
GZS16_RS23950 () 122964..123668 + 705 WP_072095570.1 type-F conjugative transfer system secretin TraK -
GZS16_RS23955 () 123668..125095 + 1428 WP_000146685.1 conjugal transfer protein TraB -
GZS16_RS23960 () 125085..125675 + 591 WP_000002787.1 conjugal transfer protein TraP -
GZS16_RS23965 () 125629..125859 + 231 WP_001352845.1 conjugal transfer protein TrbD -
GZS16_RS23970 () 125871..126122 + 252 WP_001038342.1 conjugal transfer protein TrbG -
GZS16_RS23975 () 126119..126634 + 516 WP_000809838.1 type IV conjugative transfer system lipoprotein TraV -
GZS16_RS23980 () 126769..126990 + 222 WP_001278689.1 conjugal transfer protein TraR -
GZS16_RS23985 () 127150..129777 + 2628 WP_001064245.1 type IV secretion system protein TraC -
GZS16_RS23990 () 129774..130160 + 387 WP_000099686.1 type-F conjugative transfer system protein TrbI -
GZS16_RS23995 () 130157..130789 + 633 WP_001203720.1 type-F conjugative transfer system protein TraW -
GZS16_RS24000 () 130786..131778 + 993 WP_000830180.1 conjugal transfer pilus assembly protein TraU -
GZS16_RS24005 () 131787..132425 + 639 WP_021518374.1 type-F conjugative transfer system pilin assembly protein TrbC -
GZS16_RS24010 () 132422..134230 + 1809 WP_000821840.1 type-F conjugative transfer system mating-pair stabilization protein TraN -
GZS16_RS24015 () 134257..134514 + 258 WP_000864312.1 conjugal transfer protein TrbE -
GZS16_RS24020 () 134507..135250 + 744 WP_001030378.1 type-F conjugative transfer system pilin assembly protein TraF -
GZS16_RS24025 () 135264..135605 + 342 WP_000556794.1 conjugal transfer protein TrbA -
GZS16_RS24030 () 135586..135921 - 336 WP_000415571.1 hypothetical protein -
GZS16_RS24035 () 136002..136286 + 285 WP_000624105.1 type-F conjugative transfer system pilin chaperone TraQ -
GZS16_RS24040 () 136273..136827 + 555 WP_001553830.1 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB -
GZS16_RS24045 () 136757..137131 + 375 WP_071788165.1 P-type conjugative transfer protein TrbJ -
GZS16_RS24050 () 137085..137477 + 393 WP_000164675.1 F-type conjugal transfer protein TrbF -
GZS16_RS24055 () 137464..138837 + 1374 WP_021518370.1 conjugal transfer protein TraH -
GZS16_RS24060 () 138834..141659 + 2826 WP_001553826.1 conjugal transfer mating pair stabilization protein TraG -
GZS16_RS24065 () 141656..142165 + 510 WP_000628100.1 conjugal transfer entry exclusion protein TraS -
GZS16_RS24070 () 142179..142910 + 732 WP_000850424.1 complement resistance protein TraT -
GZS16_RS24075 () 143162..145351 + 2190 WP_040110343.1 type IV conjugative transfer system coupling protein TraD -
GZS16_RS24080 () 145351..150471 + 5121 WP_013362805.1 Multifunctional conjugation protein TraI Relaxase
GZS16_RS24085 () 150491..151237 + 747 WP_023149624.1 conjugal transfer pilus acetylase TraX -
GZS16_RS24090 () 151292..151852 + 561 WP_000139363.1 fertility inhibition protein FinO -
GZS16_RS24095 () 151986..152198 + 213 WP_005012601.1 hypothetical protein -
GZS16_RS25305 () 152499..152588 - 90 Protein_180 IS1 family transposase -
GZS16_RS24105 () 152643..153320 + 678 WP_001339397.1 IS66 family transposase ISCro1 Transposase
GZS16_RS24110 () 153320..153667 + 348 WP_000624622.1 IS66 family transposase ISCro1 Transposase
GZS16_RS24115 () 153687..155258 + 1572 WP_023149734.1 IS66 family transposase ISCro1 Transposase
GZS16_RS24120 () 155296..155912 - 617 Protein_184 IS1 family transposase IS1G Transposase
GZS16_RS24125 () 156013..156195 + 183 WP_000968309.1 hypothetical protein -
- () 156334..156395 - 62 NuclAT_2 - -
- () 156334..156395 - 62 NuclAT_2 - -
- () 156334..156395 - 62 NuclAT_2 - -
- () 156334..156395 - 62 NuclAT_2 - -
GZS16_RS24130 () 156382..156588 + 207 WP_015387399.1 type I toxin-antitoxin system Hok family toxin Toxin Detail
GZS16_RS24135 () 156872..157129 + 258 WP_000083833.1 replication regulatory protein RepA -
GZS16_RS24140 () 157365..157439 + 75 WP_032336874.1 RepA leader peptide Tap -
GZS16_RS24145 () 157432..157878 + 447 Protein_189 incFII family plasmid replication initiator RepA -
GZS16_RS24150 () 157824..158306 - 483 Protein_190 VENN motif pre-toxin domain-containing protein -
GZS16_RS24155 () 159199..160419 + 1221 WP_000410951.1 arginine deiminase -
GZS16_RS24160 () 160430..161341 + 912 WP_000440183.1 carbamate kinase -
GZS16_RS24165 () 161426..162430 + 1005 WP_000154545.1 Ornithine carbamoyltransferase VF
GZS16_RS24170 () 162478..163881 + 1404 WP_000514417.1 YfcC family protein -
GZS16_RS24175 () 163962..164441 + 480 WP_001496175.1 ArgR family transcriptional regulator -
GZS16_RS24180 () 164505..164846 - 342 Protein_196 transposase -
GZS16_RS24185 () 164798..165019 - 222 WP_000080227.1 hypothetical protein -
GZS16_RS24190 () 165050..165400 - 351 WP_000624725.1 IS66 family transposase ISEc47 Transposase
GZS16_RS24195 () 165397..165759 - 363 WP_059330006.1 IS66 family insertion sequence hypothetical protein -
GZS16_RS24200 () 165868..166561 - 694 Protein_200 IS1 family transposase IS1A Transposase
GZS16_RS24205 () 166594..167172 + 579 WP_032152936.1 DUF2726 domain-containing protein -
GZS16_RS24210 () 167585..167938 - 354 WP_000005489.1 hypothetical protein -
GZS16_RS24215 () 168410..169432 - 1023 WP_000156883.1 IS110 family transposase ISSm1 Transposase
GZS16_RS24220 () 169837..170094 + 258 WP_000083821.1 replication regulatory protein RepA -

Similar MGE(s)


Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE.

Detail Organism MGE type Related TA Genome accession Coordinates Mash
distance