Detailed information of TA system TA149019

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 156334..156588
• Replicon: plasmid p32-4_A
• Accession: NZ_CP048311
• Organism: Escherichia coli strain 32-4

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: GZS16_RS24130
• Protein ID: WP_015387399.1
• Coordinates: 156382..156588 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	156334..156395 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS16_RS24095	151986..152198	+	213	WP_005012601.1	hypothetical protein	-
GZS16_RS25305	152499..152588	-	90	Protein_180	IS1 family transposase	-
GZS16_RS24105	152643..153320	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
GZS16_RS24110	153320..153667	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
GZS16_RS24115	153687..155258	+	1572	WP_023149734.1	IS66 family transposase	-
GZS16_RS24120	155296..155912	-	617	Protein_184	IS1 family transposase	-
GZS16_RS24125	156013..156195	+	183	WP_000968309.1	hypothetical protein	-
-	156334..156395	-	62	NuclAT_2	-	Antitoxin
-	156334..156395	-	62	NuclAT_2	-	Antitoxin
-	156334..156395	-	62	NuclAT_2	-	Antitoxin
-	156334..156395	-	62	NuclAT_2	-	Antitoxin
GZS16_RS24130	156382..156588	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
GZS16_RS24135	156872..157129	+	258	WP_000083833.1	replication regulatory protein RepA	-
GZS16_RS24140	157365..157439	+	75	WP_032336874.1	RepA leader peptide Tap	-
GZS16_RS24145	157432..157878	+	447	Protein_189	incFII family plasmid replication initiator RepA	-
GZS16_RS24150	157824..158306	-	483	Protein_190	VENN motif pre-toxin domain-containing protein	-
GZS16_RS24155	159199..160419	+	1221	WP_000410951.1	arginine deiminase	-
GZS16_RS24160	160430..161341	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / mph(A) / tet(B) / sul1 / qacE / aadA5 / blaCTX-M-15 / sitABCD	iucA / iucB / iucC / iucD / iutA	1..170395	170395

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T149019 WP_015387399.1 NZ_CP048311:156382-156588 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T149019 NZ_CP063916:3719985-3721307 [Klebsiella variicola]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGCACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACACGGTGTATCACTATTTTGATAACCTGCTACCCGACAGCCCGCAGGTCCGCGATCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTGGGACGGGATAGCGTAGGGGCGGTGACTCTGCTACCGCCGGG
GGAAGAGGCGCACCTCGAGGGGTTGCGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCCTACCAGT
CGGATATCCCTCTCGGGATGATTACCGGCCAGGATGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGGGCACTGGTGTATTCCGCAGGGCACCACGCCGACCACGCATATCATTAAACTGCCCATCGGCGAGAT
AAAGCAGCCTAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAGTTTCTGTGCCTGGCGCTGGCCAGGGAACTGG
GCCTGGCGGTGCCGGAGGCAGAGATTATCACCACGCCGCGGATCCGCGCGCTGGCGGTGACCCGCTTTGACCGGCGCTGG
GCGCAGGAGGGGCGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCCTTTGGCATCCCGTCGTCGATGAAATA
TGAATCTGACGGCGGGCCGGGTATTGCGGCAATCATGGCCTTTCTGCTGGGCTCCAGTGAAGCCCTGAAAGATCGCGCAG
ACTTTATGAAATTCATGGTTTTTCAGTGGCTGGTTGGGGCAACGGACGGGCATGCGAAGAACTTCTCGCTCTTTTTGCTG
CCCGGTGGCAGCTACCGTCTAACGCCGTTCTACGACATCATTTCGGCGTTTCCGGTGCTGGGAGGCACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGGAAAACAGAGATTAACGCTATCTATCCGCGTCATT
TTCTGGCAACCGCCAAAGCGGTGAACTTTCCTGGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGTTCGCCGGACGCTGCCGGCGGACTTTTCCACCCACGTCTGGCGGGCCATTACGGAAAATATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGTTATAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT149019 NZ_CP048311:c156395-156334 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin