Detailed information of TA system TA149015

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 116288..116521
• Replicon: plasmid p32-4_A
• Accession: NZ_CP048311
• Organism: Escherichia coli strain 32-4

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GZS16_RS23895
• Protein ID: WP_001312861.1
• Coordinates: 116363..116521 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	116288..116319 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS16_RS23850	111519..111725	+	207	WP_001774176.1	hypothetical protein	-
GZS16_RS25300	112135..112341	+	207	WP_000275856.1	hypothetical protein	-
GZS16_RS23860	112367..112906	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
GZS16_RS23865	112974..113207	+	234	WP_000005990.1	DUF905 family protein	-
GZS16_RS23870	113235..113432	+	198	Protein_135	hypothetical protein	-
GZS16_RS23875	113487..113921	+	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
GZS16_RS23880	113918..114637	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
GZS16_RS23885	114649..114837	-	189	WP_001299721.1	hypothetical protein	-
-	114649..114846	+	198	NuclAT_0	-	-
-	114649..114846	+	198	NuclAT_0	-	-
-	114649..114846	+	198	NuclAT_0	-	-
-	114649..114846	+	198	NuclAT_0	-	-
GZS16_RS23890	114894..116263	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	116288..116319	+	32	NuclAT_1	-	Antitoxin
-	116288..116319	+	32	NuclAT_1	-	Antitoxin
-	116288..116319	+	32	NuclAT_1	-	Antitoxin
-	116288..116319	+	32	NuclAT_1	-	Antitoxin
GZS16_RS23895	116363..116521	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
GZS16_RS23905	117442..117729	+	288	WP_000107535.1	hypothetical protein	-
GZS16_RS23910	117847..118668	+	822	WP_001234426.1	DUF945 domain-containing protein	-
GZS16_RS23915	118965..119567	-	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
GZS16_RS23920	119888..120271	+	384	WP_001151524.1	relaxosome protein TraM	-
GZS16_RS23925	120458..121147	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / mph(A) / tet(B) / sul1 / qacE / aadA5 / blaCTX-M-15 / sitABCD	iucA / iucB / iucC / iucD / iutA	1..170395	170395

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T149015 WP_001312861.1 NZ_CP048311:116363-116521 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T149015 NZ_CP063916:1107110-1107457 [Klebsiella variicola]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGGTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Nucleotide

• Length: 32 bp

>AT149015 NZ_CP048311:116288-116319 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin