MGE detailed information

MGE type: Conjugative plasmid        MGE length: 83958 bp
Accession: NZ_CP044440        Location: 1..83958         Organism: Escherichia coli strain HeN100       Replicon: plasmid pHeN100_03


Gene structure


The 5 kb flanking regions of this MGE are displayed.

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HeN100_RS01165 (1) 1..858 + 858 WP_032495102.1 incFII family plasmid replication initiator RepA -
HeN100_RS01170 (1797) 1797..2450 + 654 WP_000616807.1 CPBP family intramembrane metalloprotease -
HeN100_RS01175 (2543) 2543..2800 + 258 WP_000557619.1 type II toxin-antitoxin system antitoxin PemI -
HeN100_RS01180 (2733) 2733..3134 + 402 WP_001398199.1 type II toxin-antitoxin system toxin endoribonuclease PemK -
HeN100_RS01185 (3383) 3383..3798 + 416 Protein_4 IS1-like element IS1B family transposase -
HeN100_RS01190 (3834) 3834..4379 - 546 Protein_5 Tn3 family transposase -
HeN100_RS01195 (4445) 4445..5149 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
HeN100_RS01200 (5235) 5235..6209 + 975 WP_250664889.1 hypothetical protein -
HeN100_RS01205 (6224) 6224..7468 + 1245 WP_015060702.1 DUF1173 family protein -
HeN100_RS01210 (7479) 7479..7931 + 453 WP_004993232.1 hypothetical protein -
HeN100_RS01215 (7993) 7993..8430 - 438 WP_017480454.1 hypothetical protein -
HeN100_RS01220 (8538) 8538..9670 + 1133 WP_223155595.1 IS3-like element ISAba14 family transposase -
HeN100_RS01225 (9776) 9776..10555 + 780 WP_014386410.1 APH(3')-VI family aminoglycoside O-phosphotransferase -
HeN100_RS01230 (10764) 10764..11732 + 969 WP_005804963.1 IS30 family transposase -
HeN100_RS01235 (11833) 11833..12645 + 813 WP_004201164.1 subclass B1 metallo-beta-lactamase NDM-1 -
HeN100_RS01240 (12649) 12649..13014 + 366 WP_004201167.1 bleomycin binding protein Ble-MBL -
HeN100_RS01245 (13019) 13019..13657 + 639 WP_004201168.1 phosphoribosylanthranilate isomerase -
HeN100_RS01250 (13668) 13668..14225 - 558 Protein_17 twin-arginine translocation (TAT) pathway signal sequence domain protein -
HeN100_RS01255 (14325) 14325..14699 - 375 Protein_18 protein-disulfide reductase DsbD family protein -
HeN100_RS01260 (14704) 14704..15033 - 330 WP_004201171.1 divalent-cation tolerance protein CutA -
HeN100_RS01265 (15227) 15227..15517 + 291 WP_004201172.1 co-chaperone GroES -
HeN100_RS01270 (15573) 15573..17213 + 1641 WP_004201176.1 chaperonin GroEL -
HeN100_RS01275 (17438) 17438..18931 + 1494 WP_043907034.1 IS91 family transposase -
HeN100_RS01280 (19187) 19187..19432 - 246 Protein_23 hypothetical protein -
HeN100_RS01285 (19457) 19457..20161 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
HeN100_RS01290 (20212) 20212..20829 - 618 Protein_25 transposase zinc-binding domain-containing protein -
HeN100_RS01295 (20839) 20839..21027 - 189 WP_000957857.1 hypothetical protein -
HeN100_RS01300 (21119) 21119..21655 + 537 WP_064441864.1 recombinase family protein -
HeN100_RS01305 (21838) 21838..22698 + 861 WP_000027057.1 broad-spectrum class A beta-lactamase TEM-1 -
HeN100_RS01310 (22868) 22868..23623 + 756 WP_012372818.1 16S rRNA (guanine(1405)-N(7))-methyltransferase RmtB1 -
HeN100_RS01315 (23704) 23704..24018 - 315 Protein_30 hypothetical protein -
HeN100_RS01320 (24073) 24073..24777 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
HeN100_RS01325 (24843) 24843..24908 + 66 Protein_32 IS1 family transposase -
HeN100_RS01330 (26357) 26357..26591 + 235 Protein_33 type II toxin-antitoxin system RelE/ParE family toxin -
HeN100_RS01335 (26719) 26719..26922 + 204 Protein_34 hypothetical protein -
HeN100_RS01340 (27123) 27123..27473 - 351 WP_000361389.1 plasmid partitioning/stability family protein -
HeN100_RS01345 (27476) 27476..28438 - 963 WP_000959884.1 plasmid segregation protein ParM -
HeN100_RS01350 (28585) 28585..28878 + 294 WP_032146011.1 hypothetical protein -
HeN100_RS01355 (28955) 28955..29638 + 684 WP_000085883.1 DNA methylase -
HeN100_RS01360 (29639) 29639..29860 + 222 WP_001104881.1 hypothetical protein -
HeN100_RS01365 (29874) 29874..30308 + 435 WP_000274503.1 DUF1380 family protein -
HeN100_RS01370 (30359) 30359..31135 + 777 WP_001383963.1 hypothetical protein -
HeN100_RS01375 (31553) 31553..31978 + 426 WP_001198928.1 antirestriction protein -
HeN100_RS01380 (32025) 32025..32447 + 423 WP_000271762.1 DUF1380 family protein -
HeN100_RS01385 (32444) 32444..32635 + 192 WP_001027493.1 hypothetical protein -
HeN100_RS01390 (32949) 32949..34616 - 1668 WP_012372796.1 group II intron reverse transcriptase/maturase -
HeN100_RS01395 (35345) 35345..35499 - 155 Protein_46 hypothetical protein -
HeN100_RS01400 (36016) 36016..36246 + 231 WP_000218642.1 hypothetical protein -
HeN100_RS01405 (36298) 36298..37659 + 1362 WP_000170695.1 DUF3560 domain-containing protein -
HeN100_RS01410 (37706) 37706..38269 + 564 WP_015059007.1 class I SAM-dependent methyltransferase -
HeN100_RS01415 (38355) 38355..38810 - 456 Protein_50 hypothetical protein -
HeN100_RS01420 (39112) 39112..39639 + 528 WP_000290834.1 single-stranded DNA-binding protein -
HeN100_RS01425 (39697) 39697..39930 + 234 WP_000006003.1 DUF905 family protein -
HeN100_RS01430 (39991) 39991..42014 + 2024 Protein_53 ParB/RepB/Spo0J family partition protein -
HeN100_RS01435 (42083) 42083..42517 + 435 WP_000845953.1 conjugation system SOS inhibitor PsiB -
HeN100_RS01440 (42514) 42514..43276 + 763 Protein_55 plasmid SOS inhibition protein A -
HeN100_RS01445 (43254) 43254..43433 - 180 WP_001309233.1 hypothetical protein -
- (43245) 43245..43469 + 225 NuclAT_0 - -
- (43245) 43245..43469 + 225 NuclAT_0 - -
- (43245) 43245..43469 + 225 NuclAT_0 - -
- (43245) 43245..43469 + 225 NuclAT_0 - -
HeN100_RS01450 (43455) 43455..43604 + 150 Protein_57 plasmid maintenance protein Mok -
HeN100_RS01455 (43546) 43546..43671 + 126 WP_001372321.1 type I toxin-antitoxin system Hok family toxin Toxin Detail
HeN100_RS01460 (43990) 43990..44286 - 297 Protein_59 hypothetical protein -
HeN100_RS01465 (44586) 44586..44882 + 297 WP_001272251.1 hypothetical protein -
HeN100_RS01470 (44993) 44993..45814 + 822 WP_001234445.1 DUF932 domain-containing protein -
HeN100_RS01475 (46111) 46111..46758 - 648 WP_015059008.1 transglycosylase SLT domain-containing protein -
HeN100_RS01480 (47035) 47035..47418 + 384 WP_000124981.1 conjugal transfer relaxosome DNA-binding protein TraM -
HeN100_RS01485 (47609) 47609..48295 + 687 WP_015059009.1 PAS domain-containing protein -
HeN100_RS01490 (48389) 48389..48616 + 228 WP_001254386.1 conjugal transfer relaxosome protein TraY -
HeN100_RS01495 (48650) 48650..49015 + 366 WP_021519752.1 type IV conjugative transfer system pilin TraA -
HeN100_RS01500 (49030) 49030..49341 + 312 WP_000012106.1 type IV conjugative transfer system protein TraL -
HeN100_RS01505 (49363) 49363..49929 + 567 WP_000399794.1 type IV conjugative transfer system protein TraE -
HeN100_RS01510 (49916) 49916..50644 + 729 WP_001230787.1 type-F conjugative transfer system secretin TraK -
HeN100_RS01515 (50644) 50644..52071 + 1428 WP_032297072.1 F-type conjugal transfer pilus assembly protein TraB -
HeN100_RS01520 (52061) 52061..52651 + 591 WP_000002778.1 conjugal transfer pilus-stabilizing protein TraP -
HeN100_RS01525 (52644) 52644..52835 + 192 WP_032072783.1 conjugal transfer protein TrbD -
HeN100_RS01530 (52847) 52847..53098 + 252 WP_001038341.1 conjugal transfer protein TrbG -
HeN100_RS01535 (53095) 53095..53610 + 516 WP_000809902.1 type IV conjugative transfer system lipoprotein TraV -
HeN100_RS01540 (53745) 53745..53966 + 222 WP_001278692.1 conjugal transfer protein TraR -
HeN100_RS01545 (54126) 54126..56753 + 2628 WP_015059012.1 type IV secretion system protein TraC -
HeN100_RS01550 (56750) 56750..57136 + 387 WP_015059013.1 type-F conjugative transfer system protein TrbI -
HeN100_RS01555 (57133) 57133..57765 + 633 WP_001203735.1 type-F conjugative transfer system protein TraW -
HeN100_RS01560 (57762) 57762..58754 + 993 WP_000830838.1 conjugal transfer pilus assembly protein TraU -
HeN100_RS01565 (58781) 58781..59089 + 309 WP_000412447.1 hypothetical protein -
HeN100_RS01570 (59152) 59152..59673 + 522 WP_015059014.1 hypothetical protein -
HeN100_RS01575 (59700) 59700..60338 + 639 WP_000087532.1 type-F conjugative transfer system pilin assembly protein TrbC -
HeN100_RS01580 (60335) 60335..60706 + 372 WP_000056336.1 hypothetical protein -
HeN100_RS01585 (60732) 60732..61154 + 423 WP_001229309.1 HNH endonuclease signature motif containing protein -
HeN100_RS01590 (61151) 61151..63001 + 1851 WP_015059015.1 type-F conjugative transfer system mating-pair stabilization protein TraN -
HeN100_RS01595 (63028) 63028..63285 + 258 WP_053913405.1 conjugal transfer protein TrbE -
HeN100_RS01600 (63278) 63278..64021 + 744 WP_015059017.1 type-F conjugative transfer system pilin assembly protein TraF -
HeN100_RS01605 (64182) 64182..64418 + 237 WP_000270010.1 type-F conjugative transfer system pilin chaperone TraQ -
HeN100_RS01610 (64405) 64405..64950 + 546 WP_000059817.1 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB -
HeN100_RS01615 (64940) 64940..65254 + 315 WP_001384255.1 P-type conjugative transfer protein TrbJ -
HeN100_RS01620 (65208) 65208..65600 + 393 WP_000126338.1 F-type conjugal transfer protein TrbF -
HeN100_RS01625 (65587) 65587..66960 + 1374 WP_000944325.1 conjugal transfer pilus assembly protein TraH -
HeN100_RS01630 (66957) 66957..69773 + 2817 WP_015059018.1 conjugal transfer mating-pair stabilization protein TraG -
HeN100_RS01635 (69806) 69806..70327 + 522 WP_000632668.1 conjugal transfer entry exclusion protein TraS -
HeN100_RS01640 (70352) 70352..71083 + 732 WP_021559024.1 conjugal transfer complement resistance protein TraT -
HeN100_RS01645 (71286) 71286..72023 + 738 WP_015059020.1 hypothetical protein -
HeN100_RS01650 (72074) 72074..74299 + 2226 WP_000009363.1 type IV conjugative transfer system coupling protein TraD -
HeN100_RS01655 (74299) 74299..79569 + 5271 WP_015059021.1 conjugative transfer relaxase/helicase TraI -
HeN100_RS01660 (79589) 79589..80335 + 747 WP_000205770.1 conjugal transfer pilus acetylase TraX -
HeN100_RS01665 (80390) 80390..80950 + 561 WP_000139328.1 fertility inhibition protein FinO -
HeN100_RS01670 (81082) 81082..81282 + 201 WP_015059022.1 hypothetical protein -
HeN100_RS01675 (81668) 81668..82267 + 600 WP_032083981.1 PIN domain-containing protein -
HeN100_RS01680 (82329) 82329..82661 + 333 WP_152916585.1 hypothetical protein -
- (82862) 82862..82921 - 60 NuclAT_1 - Antitoxin Detail
- (82862) 82862..82921 - 60 NuclAT_1 - Antitoxin Detail
- (82862) 82862..82921 - 60 NuclAT_1 - Antitoxin Detail
- (82862) 82862..82921 - 60 NuclAT_1 - Antitoxin Detail
HeN100_RS01685 (82966) 82966..83115 + 150 WP_001312851.1 Hok/Gef family protein Toxin Detail
HeN100_RS01690 (83399) 83399..83647 + 249 WP_000083837.1 replication regulatory protein RepA -

Similar MGE(s)


Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE.

Detail Organism MGE type Related TA Genome accession Coordinates Mash
distance