Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 82862..83115 Replicon plasmid pHeN100_03
Accession NZ_CP044440
Organism Escherichia coli strain HeN100

Toxin (Protein)


Gene name srnB Uniprot ID G9G1E3
Locus tag HeN100_RS01685 Protein ID WP_001312851.1
Coordinates 82966..83115 (+) Length 50 a.a.

Antitoxin (RNA)


Gene name sok
Locus tag -
Coordinates 82862..82921 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HeN100_RS01660 (79589) 79589..80335 + 747 WP_000205770.1 conjugal transfer pilus acetylase TraX -
HeN100_RS01665 (80390) 80390..80950 + 561 WP_000139328.1 fertility inhibition protein FinO -
HeN100_RS01670 (81082) 81082..81282 + 201 WP_015059022.1 hypothetical protein -
HeN100_RS01675 (81668) 81668..82267 + 600 WP_032083981.1 PIN domain-containing protein -
HeN100_RS01680 (82329) 82329..82661 + 333 WP_152916585.1 hypothetical protein -
- (82862) 82862..82921 - 60 NuclAT_1 - Antitoxin
- (82862) 82862..82921 - 60 NuclAT_1 - Antitoxin
- (82862) 82862..82921 - 60 NuclAT_1 - Antitoxin
- (82862) 82862..82921 - 60 NuclAT_1 - Antitoxin
HeN100_RS01685 (82966) 82966..83115 + 150 WP_001312851.1 Hok/Gef family protein Toxin
HeN100_RS01690 (83399) 83399..83647 + 249 WP_000083837.1 replication regulatory protein RepA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-VI / blaNDM-1 / blaTEM-1B / rmtB htpB 1..83958 83958


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(4-45)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5542.67 Da        Isoelectric Point: 8.7678

>T140956 WP_001312851.1 NZ_CP044440:82966-83115 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T140956 NZ_CP059583:420672-420824 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Antitoxin


Download         Length: 60 bp

>AT140956 NZ_CP044440:c82921-82862 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C8K5

Antitoxin

Download structure file

References