Detailed information of TA system TA140953

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 43402..43671
• Replicon: plasmid pHeN100_03
• Accession: NZ_CP044440
• Organism: Escherichia coli strain HeN100

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HeN100_RS01455
• Protein ID: WP_001372321.1
• Coordinates: 43546..43671 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	43402..43467 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HeN100_RS01420	39112..39639	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HeN100_RS01425	39697..39930	+	234	WP_000006003.1	DUF905 family protein	-
HeN100_RS01430	39991..42014	+	2024	Protein_53	ParB/RepB/Spo0J family partition protein	-
HeN100_RS01435	42083..42517	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HeN100_RS01440	42514..43276	+	763	Protein_55	plasmid SOS inhibition protein A	-
-	43245..43469	+	225	NuclAT_0	-	-
-	43245..43469	+	225	NuclAT_0	-	-
-	43245..43469	+	225	NuclAT_0	-	-
-	43245..43469	+	225	NuclAT_0	-	-
HeN100_RS01445	43254..43433	-	180	WP_001309233.1	hypothetical protein	-
-	43402..43467	+	66	-	-	Antitoxin
HeN100_RS01450	43455..43604	+	150	Protein_57	plasmid maintenance protein Mok	-
HeN100_RS01455	43546..43671	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HeN100_RS01460	43990..44286	-	297	Protein_59	hypothetical protein	-
HeN100_RS01465	44586..44882	+	297	WP_001272251.1	hypothetical protein	-
HeN100_RS01470	44993..45814	+	822	WP_001234445.1	DUF932 domain-containing protein	-
HeN100_RS01475	46111..46758	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
HeN100_RS01480	47035..47418	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HeN100_RS01485	47609..48295	+	687	WP_015059009.1	PAS domain-containing protein	-
HeN100_RS01490	48389..48616	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-VI / blaNDM-1 / blaTEM-1B / rmtB	htpB	1..83958	83958

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T140953 WP_001372321.1 NZ_CP044440:43546-43671 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T140953 NZ_CP059582:2102617-2102868 [Xanthomonas oryzae pv. oryzae]
ATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGGGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT140953 NZ_CP044440:43402-43467 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Antitoxin