MGE detailed information
MGE type: Conjugative plasmid MGE length: 149317 bp
Accession: NZ_CP035517 Location: 1..149317 Organism: Escherichia coli strain U14A Replicon: plasmid pU14A_A
Accession: NZ_CP035517 Location: 1..149317 Organism: Escherichia coli strain U14A Replicon: plasmid pU14A_A
Gene structure
The 5 kb flanking regions of this MGE are displayed.
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| U14A_RS25215 () | 349..951 | + | 603 | WP_000243712.1 | hypothetical protein | VF |
| U14A_RS25220 () | 1248..2069 | - | 822 | WP_001234469.1 | DUF945 domain-containing protein | - |
| U14A_RS25225 () | 2188..2475 | - | 288 | WP_000107535.1 | hypothetical protein | - |
| U14A_RS27165 () | 2500..2706 | - | 207 | WP_000275859.1 | hypothetical protein | - |
| U14A_RS27170 () | 2619..2954 | - | 336 | WP_013023876.1 | hypothetical protein | - |
| U14A_RS25245 () | 3397..3555 | - | 159 | WP_001312861.1 | type I toxin-antitoxin system Hok family toxin | Toxin Detail |
| - () | 3599..3639 | - | 41 | NuclAT_1 | - | Antitoxin Detail |
| - () | 3599..3639 | - | 41 | NuclAT_1 | - | Antitoxin Detail |
| - () | 3599..3639 | - | 41 | NuclAT_1 | - | Antitoxin Detail |
| - () | 3599..3639 | - | 41 | NuclAT_1 | - | Antitoxin Detail |
| - () | 5083..5269 | - | 187 | NuclAT_0 | - | - |
| - () | 5083..5269 | - | 187 | NuclAT_0 | - | - |
| - () | 5083..5269 | - | 187 | NuclAT_0 | - | - |
| - () | 5083..5269 | - | 187 | NuclAT_0 | - | - |
| U14A_RS25255 () | 5281..6000 | - | 720 | WP_001276232.1 | plasmid SOS inhibition protein A | - |
| U14A_RS25260 () | 5997..6431 | - | 435 | WP_000845940.1 | conjugation system SOS inhibitor PsiB | - |
| U14A_RS25265 () | 6486..8444 | - | 1959 | WP_000117179.1 | ParB/RepB/Spo0J family partition protein | - |
| U14A_RS25270 () | 8510..8743 | - | 234 | WP_000005990.1 | DUF905 family protein | - |
| U14A_RS25275 () | 8806..9345 | - | 540 | WP_000290841.1 | single-stranded DNA-binding protein | - |
| U14A_RS27175 () | 9490..9825 | - | 336 | WP_013023883.1 | hypothetical protein | - |
| U14A_RS25300 () | 9988..10224 | - | 237 | WP_032153960.1 | hypothetical protein | - |
| U14A_RS25305 () | 10250..10813 | - | 564 | WP_001348621.1 | class I SAM-dependent methyltransferase | - |
| U14A_RS25310 () | 10861..12222 | - | 1362 | WP_000170714.1 | DUF3560 domain-containing protein | - |
| U14A_RS25315 () | 12274..12504 | - | 231 | WP_000218642.1 | hypothetical protein | - |
| U14A_RS25325 () | 13539..13730 | - | 192 | WP_001027516.1 | hypothetical protein | - |
| U14A_RS25330 () | 13727..14149 | - | 423 | WP_000271753.1 | DUF1380 family protein | - |
| U14A_RS25335 () | 14196..14498 | - | 303 | WP_001671341.1 | antirestriction protein | - |
| U14A_RS25345 () | 15038..15814 | - | 777 | WP_001753849.1 | hypothetical protein | - |
| U14A_RS25350 () | 15865..16299 | - | 435 | WP_000274437.1 | DUF1380 family protein | - |
| U14A_RS25355 () | 16313..16534 | - | 222 | WP_001104873.1 | hypothetical protein | - |
| U14A_RS25360 () | 16535..17218 | - | 684 | WP_000086185.1 | DNA methylase | - |
| U14A_RS25365 () | 17295..17599 | - | 305 | Protein_25 | hypothetical protein | - |
| U14A_RS25370 () | 17603..18505 | - | 903 | WP_001348615.1 | DUF1281 domain-containing protein | - |
| U14A_RS25385 () | 19372..20343 | - | 972 | WP_000817036.1 | ParB/RepB/Spo0J family plasmid partition protein | - |
| U14A_RS25390 () | 20343..21509 | - | 1167 | WP_000772446.1 | Sporulation initiation inhibitor protein Soj | VF |
| U14A_RS25395 () | 22097..22852 | - | 756 | WP_000852146.1 | replication initiation protein RepE | - |
| U14A_RS25400 () | 23626..24432 | - | 807 | WP_000016982.1 | site-specific integrase | - |
| U14A_RS25405 () | 24433..24738 | - | 306 | WP_001159868.1 | type II toxin-antitoxin system toxin CcdB | Toxin Detail |
| U14A_RS25410 () | 24740..24958 | - | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin Detail |
| U14A_RS25415 () | 25666..26661 | + | 996 | WP_000246636.1 | hypothetical protein | - |
| U14A_RS25420 () | 26665..27597 | + | 933 | WP_000991832.1 | hypothetical protein | - |
| U14A_RS25425 () | 27734..28431 | - | 698 | Protein_35 | IS1 family transposase IS1G | Transposase |
| U14A_RS25430 () | 28645..31761 | - | 3117 | WP_001553854.1 | HsdR family type I site-specific deoxyribonuclease | - |
| U14A_RS25435 () | 31883..33166 | - | 1284 | WP_001617890.1 | restriction endonuclease subunit S | - |
| U14A_RS25440 () | 33163..34719 | - | 1557 | WP_001617892.1 | type I restriction-modification system subunit M | - |
| U14A_RS25445 () | 34902..35123 | + | 222 | WP_001190712.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin Detail |
| U14A_RS25450 () | 35123..35503 | + | 381 | WP_001216034.1 | type II toxin-antitoxin system death-on-curing family toxin | Toxin Detail |
| U14A_RS25455 () | 35508..35687 | + | 180 | WP_001513661.1 | hypothetical protein | - |
| U14A_RS25460 () | 35715..36074 | + | 360 | WP_001513660.1 | hypothetical protein | - |
| U14A_RS25465 () | 35998..36411 | + | 414 | Protein_43 | IS3 family transposase ISLad1 | Transposase |
| U14A_RS25470 () | 36361..36678 | - | 318 | WP_001513659.1 | hypothetical protein | - |
| U14A_RS25475 () | 36906..37886 | - | 981 | WP_000019407.1 | IS5 family transposase IS5D | Transposase |
| U14A_RS25480 () | 38130..39533 | + | 1404 | WP_001373486.1 | S-methylmethionine permease | - |
| U14A_RS25485 () | 39520..40452 | + | 933 | WP_000081352.1 | homocysteine S-methyltransferase | - |
| U14A_RS25490 () | 40561..40890 | - | 330 | Protein_48 | TOBE domain-containing protein | - |
| U14A_RS25495 () | 40906..41603 | - | 698 | Protein_49 | IS1 family transposase IS1R | Transposase |
| U14A_RS25500 () | 41661..41909 | + | 249 | Protein_50 | abortive infection family protein | - |
| U14A_RS25505 () | 42278..43009 | - | 732 | WP_001617898.1 | hypothetical protein | - |
| U14A_RS25515 () | 43795..44772 | + | 978 | WP_000361610.1 | RepB family plasmid replication initiator protein | - |
| U14A_RS25520 () | 45057..45797 | - | 741 | WP_001066941.1 | tyrosine-type recombinase/integrase | - |
| U14A_RS25525 () | 45918..46106 | - | 189 | WP_001309252.1 | hypothetical protein | - |
| U14A_RS25530 () | 46473..47642 | + | 1170 | WP_001072358.1 | hypothetical protein | - |
| U14A_RS25535 () | 47868..48043 | - | 176 | Protein_56 | IS3 family transposase | - |
| U14A_RS25540 () | 48489..48761 | - | 273 | WP_001322642.1 | Major pilu subunit operon regulatory protein PapB | VF |
| U14A_RS25545 () | 49286..49722 | - | 437 | Protein_58 | IS3 family transposase IS2 | Transposase |
| U14A_RS25550 () | 50004..51974 | + | 1971 | WP_001298664.1 | Hemin receptor | VF |
| U14A_RS25555 () | 51981..52772 | + | 792 | WP_000977394.1 | DUF4198 domain-containing protein | - |
| U14A_RS25560 () | 52835..53443 | - | 609 | Protein_61 | IS66 family transposase ISEc22 | Transposase |
| U14A_RS25565 () | 53511..54293 | - | 783 | WP_001300609.1 | IS21 family transposase IS100kyp | Transposase |
| U14A_RS25570 () | 54290..55312 | - | 1023 | WP_001310017.1 | IS21 family transposase IS100kyp | Transposase |
| U14A_RS25575 () | 55398..56343 | - | 946 | Protein_64 | IS66 family transposase ISEc8 | Transposase |
| U14A_RS25580 () | 56392..56739 | - | 348 | WP_000612626.1 | IS66 family transposase ISKpn24 | Transposase |
| U14A_RS25585 () | 56736..57140 | - | 405 | WP_000839179.1 | IS66 family transposase ISEc22 | Transposase |
| U14A_RS25590 () | 57169..57552 | - | 384 | Protein_67 | IS3 family transposase IS629 | Transposase |
| U14A_RS25595 () | 57642..59150 | - | 1509 | WP_001189113.1 | group II intron reverse transcriptase/maturase | - |
| U14A_RS25605 () | 59823..60562 | - | 740 | Protein_69 | IS3 family transposase IS629 | Transposase |
| U14A_RS25610 () | 60588..60893 | + | 306 | Protein_70 | IS91 family transposase ISSbo1 | Transposase |
| U14A_RS25615 () | 61416..62591 | - | 1176 | WP_001020413.1 | putative protein YncE | VF |
| U14A_RS25625 () | 62660..64921 | - | 2262 | WP_001100763.1 | TonB-dependent receptor | - |
| U14A_RS25630 () | 65090..65866 | - | 777 | WP_000981091.1 | energy transducer TonB | - |
| U14A_RS25635 () | 65874..66749 | - | 876 | WP_001224623.1 | ChaN family lipoprotein | - |
| U14A_RS25640 () | 67081..67628 | - | 548 | Protein_75 | primase alpha helix C-terminal domain-containing protein | - |
| U14A_RS25665 () | 69200..69535 | - | 336 | WP_001080732.1 | colicin transporter | - |
| U14A_RS25670 () | 69664..70011 | + | 348 | WP_000142452.1 | hypothetical protein | - |
| U14A_RS25675 () | 70031..70540 | - | 510 | WP_000194542.1 | hypothetical protein | - |
| U14A_RS25680 () | 70537..70797 | - | 261 | WP_000371882.1 | hypothetical protein | - |
| U14A_RS25685 () | 70899..71638 | + | 740 | Protein_80 | IS3 family transposase IS629 | Transposase |
| U14A_RS27180 () | 72311..72625 | + | 315 | Protein_81 | IS5 family transposase ISKpn26 | Transposase |
| U14A_RS27185 () | 72626..72799 | + | 174 | Protein_82 | IS5 family transposase ISKpn26 | Transposase |
| U14A_RS25700 () | 72949..73582 | - | 634 | Protein_83 | histidine-type phosphatase | - |
| U14A_RS25705 () | 73704..74189 | - | 486 | WP_000874189.1 | hypothetical protein | - |
| U14A_RS25710 () | 74214..74699 | - | 486 | WP_001267176.1 | TlpA family protein disulfide reductase | - |
| U14A_RS25715 () | 74686..75381 | - | 696 | WP_000117262.1 | Lipoprotein-releasing system ATP-binding protein LolD | VF |
| U14A_RS25720 () | 75386..76516 | - | 1131 | WP_000729218.1 | ABC transporter permease | - |
| U14A_RS25725 () | 76506..77789 | - | 1284 | WP_000964653.1 | ABC transporter permease | - |
| U14A_RS25730 () | 77792..79171 | - | 1380 | WP_000119836.1 | DUF2318 domain-containing protein | - |
| U14A_RS25735 () | 79275..79802 | - | 528 | WP_000178050.1 | iron transporter | - |
| U14A_RS25740 () | 79843..81729 | - | 1887 | WP_000118029.1 | FTR1 family iron permease | - |
| U14A_RS25750 () | 82076..82891 | - | 816 | WP_012372823.1 | Na(+)-translocating NADH-quinone reductase subunit C | - |
| U14A_RS25755 () | 83074..83580 | - | 507 | WP_000949452.1 | protein disulfide oxidoreductase | - |
| U14A_RS25760 () | 83570..83728 | - | 159 | WP_000449408.1 | DsbA family protein | - |
| U14A_RS27190 () | 83878..83991 | - | 114 | Protein_95 | IS6 family transposase | - |
| U14A_RS25775 () | 84191..84864 | + | 674 | Protein_96 | IS1 family transposase IS1X2 | Transposase |
| U14A_RS25780 () | 84917..85621 | - | 705 | WP_001067858.1 | IS6 family transposase IS15DI | Transposase |
| U14A_RS25785 () | 85680..86429 | - | 750 | Protein_98 | IS91 family transposase ISAzo26 | Transposase |
| U14A_RS25790 () | 86587..87060 | + | 474 | WP_001389366.1 | trimethoprim-resistant dihydrofolate reductase DfrA17 | - |
| U14A_RS25795 () | 87191..87979 | + | 789 | WP_000503573.1 | Streptomycin 3''-adenylyltransferase | ARG |
| U14A_RS25800 () | 88185..88532 | + | 348 | WP_000679427.1 | Multidrug transporter EmrE | ARG |
| U14A_RS25805 () | 88526..89365 | + | 840 | WP_000259031.1 | Dihydropteroate synthase | ARG |
| U14A_RS25815 () | 89493..89696 | + | 204 | WP_000376616.1 | hypothetical protein | - |
| U14A_RS25820 () | 89852..91057 | + | 1206 | WP_000184001.1 | chromate efflux transporter | - |
| U14A_RS25825 () | 91068..91373 | + | 306 | WP_000130000.1 | hypothetical protein | Relaxase |
| U14A_RS27010 () | 91417..91482 | + | 66 | Protein_106 | EAL domain-containing protein | - |
| U14A_RS25835 () | 91600..92364 | + | 765 | WP_001389365.1 | IS6 family transposase IS6100 | Transposase |
| U14A_RS25850 () | 92857..93441 | - | 585 | WP_001137892.1 | TetR family transcriptional regulator | - |
| U14A_RS25855 () | 93441..94679 | - | 1239 | WP_000004159.1 | MFS transporter | - |
| U14A_RS25860 () | 94676..95581 | - | 906 | WP_000219391.1 | hypothetical protein | ARG |
| U14A_RS25870 () | 95703..96407 | - | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| U14A_RS25875 () | 96468..96644 | + | 177 | Protein_112 | replication protein C | - |
| U14A_RS25885 () | 96952..97767 | + | 816 | WP_001043260.1 | Dihydropteroate synthase | ARG |
| U14A_RS25890 () | 97828..98631 | + | 804 | WP_001082319.1 | Aminoglycoside 3'-phosphotransferase | ARG |
| U14A_RS25895 () | 98631..99467 | + | 837 | WP_000480972.1 | hypothetical protein | ARG |
| U14A_RS25900 () | 99521..99757 | + | 237 | WP_042005022.1 | hypothetical protein | - |
| U14A_RS25905 () | 99789..100415 | - | 627 | WP_031606906.1 | tetracycline resistance transcriptional repressor TetR(A) | - |
| U14A_RS25910 () | 100521..101720 | + | 1200 | WP_000804064.1 | Tetracycline resistance protein, class C | ARG |
| U14A_RS25915 () | 101752..102612 | - | 861 | WP_000493383.1 | EamA family transporter | - |
| U14A_RS25920 () | 102621..103127 | - | 507 | Protein_120 | transposase | - |
| U14A_RS25930 () | 103180..103884 | - | 705 | WP_001067858.1 | IS6 family transposase IS15DI | Transposase |
| U14A_RS25935 () | 104183..105043 | + | 861 | WP_000027057.1 | Beta-lactamase TEM | ARG |
| U14A_RS25940 () | 105193..105291 | - | 99 | Protein_123 | IS1 family transposase | - |
| U14A_RS25945 () | 105320..106630 | - | 1311 | WP_001262765.1 | DUF262 domain-containing protein | - |
| U14A_RS25950 () | 106915..107316 | - | 402 | WP_001398199.1 | type II toxin-antitoxin system toxin endoribonuclease PemK | - |
| U14A_RS25955 () | 107249..107506 | - | 258 | WP_000557619.1 | type II toxin-antitoxin system antitoxin PemI | - |
| U14A_RS25960 () | 107599..108252 | - | 654 | WP_000616807.1 | CPBP family intramembrane metalloprotease | - |
| U14A_RS25970 () | 109192..110049 | - | 858 | WP_001617855.1 | incFII family plasmid replication initiator RepA | - |
| U14A_RS25975 () | 110042..110116 | - | 75 | WP_031943482.1 | RepA leader peptide Tap | - |
| U14A_RS27195 () | 110113..110247 | - | 135 | Protein_130 | protein CopA/IncA | - |
| U14A_RS25985 () | 110353..110607 | - | 255 | WP_000083850.1 | replication regulatory protein RepA | - |
| U14A_RS25990 () | 110904..111038 | - | 135 | WP_023144756.1 | type I toxin-antitoxin system Hok family toxin | Toxin Detail |
| - () | 111083..111143 | + | 61 | NuclAT_2 | - | Antitoxin Detail |
| - () | 111083..111143 | + | 61 | NuclAT_2 | - | Antitoxin Detail |
| - () | 111083..111143 | + | 61 | NuclAT_2 | - | Antitoxin Detail |
| - () | 111083..111143 | + | 61 | NuclAT_2 | - | Antitoxin Detail |
| U14A_RS27015 () | 111110..111396 | - | 287 | Protein_133 | DUF2726 domain-containing protein | - |
| U14A_RS26000 () | 111474..113087 | - | 1614 | WP_000080195.1 | IS66 family transposase ISEc23 | Transposase |
| U14A_RS26005 () | 113118..113468 | - | 351 | WP_000624722.1 | IS66 family transposase ISEc23 | Transposase |
| U14A_RS26010 () | 113465..113890 | - | 426 | WP_000422741.1 | IS66 family transposase ISEc23 | Transposase |
| U14A_RS26020 () | 114449..114661 | - | 213 | WP_013023861.1 | hypothetical protein | - |
| U14A_RS26025 () | 114792..115352 | - | 561 | WP_000139341.1 | fertility inhibition protein FinO | - |
| U14A_RS26030 () | 115407..116153 | - | 747 | WP_000205718.1 | conjugal transfer pilus acetylase TraX | - |
| U14A_RS26035 () | 116173..121443 | - | 5271 | WP_001617867.1 | Multifunctional conjugation protein TraI | Relaxase |
| U14A_RS26040 () | 121443..123641 | - | 2199 | WP_024623315.1 | type IV conjugative transfer system coupling protein TraD | - |
| U14A_RS26045 () | 123638..124417 | - | 780 | WP_000199914.1 | hypothetical protein | - |
| U14A_RS26050 () | 124620..125351 | - | 732 | WP_000850429.1 | complement resistance protein TraT | - |
| U14A_RS26055 () | 125383..125880 | - | 498 | WP_000605862.1 | entry exclusion protein | - |
| U14A_RS26060 () | 125899..128718 | - | 2820 | WP_001007062.1 | conjugal transfer mating pair stabilization protein TraG | - |
| U14A_RS26065 () | 128715..130088 | - | 1374 | WP_000944328.1 | conjugal transfer protein TraH | - |
| U14A_RS26070 () | 130075..130500 | - | 426 | WP_001348758.1 | F-type conjugal transfer protein TrbF | - |
| U14A_RS26075 () | 130448..130795 | - | 348 | WP_071571857.1 | P-type conjugative transfer protein TrbJ | - |
| U14A_RS26080 () | 130725..131270 | - | 546 | WP_000059829.1 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | - |
| U14A_RS26085 () | 131257..131541 | - | 285 | WP_001617873.1 | type-F conjugative transfer system pilin chaperone TraQ | - |
| U14A_RS26090 () | 131668..131988 | - | 321 | WP_001348757.1 | conjugal transfer protein TrbA | - |
| U14A_RS26095 () | 132036..132733 | - | 698 | Protein_152 | IS1 family transposase IS1G | Transposase |
| U14A_RS26100 () | 132888..133868 | - | 981 | WP_000019450.1 | IS5 family transposase ISKpn26 | Transposase |
| U14A_RS26105 () | 133998..134741 | - | 744 | WP_001030401.1 | type-F conjugative transfer system pilin assembly protein TraF | - |
| U14A_RS26110 () | 134734..134991 | - | 258 | WP_000864347.1 | conjugal transfer protein TrbE | - |
| U14A_RS26115 () | 135018..136868 | - | 1851 | WP_000821866.1 | type-F conjugative transfer system mating-pair stabilization protein TraN | - |
| U14A_RS26120 () | 136865..137503 | - | 639 | WP_001080259.1 | type-F conjugative transfer system pilin assembly protein TrbC | - |
| U14A_RS26125 () | 137512..137817 | - | 306 | WP_000224413.1 | hypothetical protein | - |
| U14A_RS26130 () | 137847..138839 | - | 993 | WP_000830190.1 | conjugal transfer pilus assembly protein TraU | - |
| U14A_RS26135 () | 138836..139468 | - | 633 | WP_001203727.1 | type-F conjugative transfer system protein TraW | - |
| U14A_RS26140 () | 139465..139851 | - | 387 | WP_000214084.1 | type-F conjugative transfer system protein TrbI | - |
| U14A_RS26145 () | 139848..142475 | - | 2628 | WP_001064258.1 | type IV secretion system protein TraC | - |
| U14A_RS26150 () | 142635..142856 | - | 222 | WP_001278694.1 | conjugal transfer protein TraR | - |
| U14A_RS26155 () | 142991..143506 | - | 516 | WP_000809886.1 | type IV conjugative transfer system lipoprotein TraV | - |
| U14A_RS26160 () | 143506..143865 | - | 360 | WP_001617876.1 | conjugal transfer protein TrbD | - |
| U14A_RS26165 () | 143804..144391 | - | 588 | WP_013023871.1 | conjugal transfer protein TraP | - |
| U14A_RS26170 () | 144360..145811 | - | 1452 | WP_000146687.1 | conjugal transfer protein TraB | - |
| U14A_RS26175 () | 145811..146515 | - | 705 | WP_148725207.1 | type-F conjugative transfer system secretin TraK | - |
| U14A_RS26180 () | 146526..147092 | - | 567 | WP_000399791.1 | type IV conjugative transfer system protein TraE | - |
| U14A_RS26185 () | 147114..147425 | - | 312 | WP_000012107.1 | type IV conjugative transfer system protein TraL | - |
| U14A_RS26190 () | 147430..147792 | - | 363 | WP_000338606.1 | type IV conjugative transfer system pilin TraA | - |
| U14A_RS26195 () | 147826..148053 | - | 228 | WP_001254388.1 | conjugal transfer relaxosome protein TraY | - |
| U14A_RS26200 () | 148141..148818 | - | 678 | WP_001348626.1 | PAS domain-containing protein | - |
Similar MGE(s)
Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE.
| Detail | Organism | MGE type | Related TA | Genome accession | Coordinates | Mash distance |
|---|