Detailed information of TA system TA118059

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 110904..111143
• Replicon: plasmid pU14A_A
• Accession: NZ_CP035517
• Organism: Escherichia coli strain U14A

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: U14A_RS25990
• Protein ID: WP_023144756.1
• Coordinates: 110904..111038 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	111083..111143 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U14A_RS25950	106915..107316	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
U14A_RS25955	107249..107506	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
U14A_RS25960	107599..108252	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
U14A_RS25970	109192..110049	-	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
U14A_RS25975	110042..110116	-	75	WP_031943482.1	RepA leader peptide Tap	-
U14A_RS27195	110113..110247	-	135	Protein_130	protein CopA/IncA	-
U14A_RS25985	110353..110607	-	255	WP_000083850.1	replication regulatory protein RepA	-
U14A_RS25990	110904..111038	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	111083..111143	+	61	NuclAT_2	-	Antitoxin
-	111083..111143	+	61	NuclAT_2	-	Antitoxin
-	111083..111143	+	61	NuclAT_2	-	Antitoxin
-	111083..111143	+	61	NuclAT_2	-	Antitoxin
U14A_RS27015	111110..111396	-	287	Protein_133	DUF2726 domain-containing protein	-
U14A_RS26000	111474..113087	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
U14A_RS26005	113118..113468	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
U14A_RS26010	113465..113890	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
U14A_RS26020	114449..114661	-	213	WP_013023861.1	hypothetical protein	-
U14A_RS26025	114792..115352	-	561	WP_000139341.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaTEM-1B	senB	1..149317	149317

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T118059 WP_023144756.1 NZ_CP035517:c111038-110904 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T118059 NZ_CP035517:c111038-110904 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT118059 NZ_CP035517:111083-111143 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin